ScerGAL4TrpA1.SH is expressed in the 4 AC thermosensory neurons, in 10 other cells in the brain and subesophageal ganglion and in 8 cells in the thoracico-abdominal ganglion. Co-expression of ScerGAL4TrpA1.SH with pre- and postsynaptic markers labels presynaptic terminals in the dorsal protocerebrum and postsynapses in the cell body of the AC neurons, at the subesophageal ganglion and at the dorsolateral protocerebrum.