FB2026_02 , released June 18, 2026
Allele: Scer\GAL4TrpA1.SH
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General Information
Symbol
Scer\GAL4TrpA1.SH
Species
S. cerevisiae
Name
FlyBase ID
FBal0220315
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{TrpA1-GAL4.SH}
Also Known As
dTrpA1SH-GAL4, TrpA1SH-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Hamada et al., 2008)
Progenitor genotype
Carried in construct
P{TrpA1-GAL4.SH}
Cytology
Description

A 2.5kb fragment from upstream of the TrpA1 start codon is fused to sequence encoding a GAL4 driver. This is followed by a 1.7kb fragment consisting of TrpA1 sequences from exon 1 to the fourth intron.

(Hamada et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
TrpA1
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      AC thermosensory neuron
      (Jin et al., 2021, Shih and Chiang, 2011, Hamada et al., 2008)
      adult brain | restricted
      (Shih and Chiang, 2011)
      gnathal ganglion | restricted
      (Shih and Chiang, 2011)
      adult ventral nerve cord | restricted
      (Shih and Chiang, 2011)
      Additional Information
      Statement
      Reference

      ScerGAL4TrpA1.SH is expressed in the 4 AC thermosensory neurons, in 10 other cells in the brain and subesophageal ganglion and in 8 cells in the thoracico-abdominal ganglion. Co-expression of ScerGAL4TrpA1.SH with pre- and postsynaptic markers labels presynaptic terminals in the dorsal protocerebrum and postsynapses in the cell body of the AC neurons, at the subesophageal ganglion and at the dorsolateral protocerebrum.

      (Shih and Chiang, 2011)
       
      Marker for
      Reflects expression of
      TrpA1
      (Jin et al., 2021, Shih and Chiang, 2011, Hamada et al., 2008)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Ctet\tetXTNT-LC.UAS) of Scer\GAL4TrpA1.SH cells in adults significantly reduces aversive temperature, but not shock, olfactory memory, compared to controls.

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4TrpA1.SH cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces aversive temperature olfactory memory, compared to controls.

      (Galili et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4TrpA1.SH
      Name Synonyms
      Secondary FlyBase IDs
        References (11)