FB2026_03 , released September 17, 2026
Recombinant Construct: PBac{GreenEye.nanosGAL4}
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General Information
Symbol
PBac{GreenEye.nanosGAL4}
FlyBase ID
FBtp0056000
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4VP16.nanos.UTR
Also Known As
pBac{GreenEye.nosGal4}, nanos-Gal4, nos-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Avic\GFPE.3xP3
w+mW.hs
Component allele Scer\GAL4VP16.nanos.UTR
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
GAL4::VP16
Tagged with
Tag:FLAG
Description

nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.

(Van Doren et al., 1998)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 6
organism | posterior
(Van Doren et al., 1998)
embryonic stage 9
germline cell
(Van Doren et al., 1998)
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 15
germline cell
(Jenkins et al., 2003)
adult stage
germline stem cell
(Wang et al., 2020)
oogenesis & adult stage | female
female germline cell
(Moschall et al., 2019)
oogenesis
female germline stem cell
(Rorth, 1998)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 6
organism | posterior
(Van Doren et al., 1998)
primordial germ cell
(Van Doren et al., 1998)
embryonic stage 9
germline cell
(Van Doren et al., 1998)
Additional Information
Statement
Reference

Drives expression in female germline stem cells at all developmental stages. Commonly used together with Scer\GAL4nanos.PG and Scer\GAL4VP16.otu to form a combination referred to as the "maternal triple driver" (MTD-GAL4).

(FlyBase Curators, 2017)

Expression is observed in female germline stem cells at all stages and at a high level..

(Rorth, 1998)

On the basis of expression of P{UAS-lacZ.B} target construct, early expression is detected in posterior somatic cells, but not in pole cells. Posterior expression exhibits a posterior-to-anterior concentration gradient. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.

(Van Doren et al., 1998)

In the early embryo, ScerGAL4nos.UTR.T:HsimVP16 RNA detected in pole cells and subsequently in germ cells. However, expression ofthe GAL4::VP16-responsive target construct, P{UAS-lacZ.B}, differs. Early expression is detected in posterior somatic cells, but not in pole cells. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.

(Van Doren et al., 1998)
 
Marker for
female germline stem cell
(Rorth, 1998)
germline stem cell
(Wang et al., 2020)
Reflects expression of
nanos
(Moschall et al., 2019, Rorth, 1998)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
Bloomington
32179
w*; PBac{GreenEye.nanosGAL4}Dmel2
32180
w*; PBac{GreenEye.nanosGAL4}Dmel6
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
Reported As
Symbol Synonym
GreenEye-nanos-Gal4
(Kahney et al., 2021)
NosGAL4 VP16
(Sarkar et al., 2026)
PBac{GreenEye.nanosGAL4}
(FlyBase, 1996-)
PBac{GreenEye.nosGAL4}
(Laws et al., 2015, FlyBase, 1996-)
nanos-GAL4
(Xia et al., 2022)
nanos-Gal4
(Shalaby et al., 2017)
nanosGAL4-VP16
(Fajner et al., 2021)
nos(green eye)-Gal4
(Chandrasekhara et al., 2023)
nos-Gal4
(Asif-Laidin et al., 2023, Laws et al., 2015)
nos-Gal4::VP16
(Laws et al., 2015)
pBac(3xP3-EGFPafm)::nos-Gal4::(pW8-)
(Eisman and Kaufman, 2013)
pBac(3xP3-EGFPafm)::nos-Gal4::(pW8-mini-white)
(Holtzman et al., 2010)
pBac{GreenEye.nosGal4}
(Holtzman et al., 2010)
Secondary FlyBase IDs
    References (14)