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General Information
Symbol
PBac{GreenEye.nosGAL4}
FlyBase ID
FBtp0056000
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Tni\piggyBac
Construct components
Product class / Tool use(s)
Component allele Scer\GAL4VP16.nos.UTR
Product class / Tool use(s)
Regulatory region(s)
Description

nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
binary
component
molecular
tag
selectable
marker
selectable
marker
Component Alleles
Molecular data

nos coding sequences within nosgnosb are replaced with sequences encoding the Tag:FLAG epitope and a GAL4::VP16 driver. The construct contains approximately 700bp of the nos promoter, the nos 5' and 3' UTRs and approximately 500bp of genomic sequences 3' of nos.

Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in female germline stem cells at all developmental stages.

On the basis of expression of P{UAS-lacZ.B} target construct, early expression is detected in posterior somatic cells, but not in pole cells. Posterior expression exhibits a posterior-to-anterior concentration gradient. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.

In the early embryo, Scer\GAL4nos.UTR.T:Hsim\VP16 RNA detected in pole cells and subsequently in germ cells. However, expression ofthe GAL4::VP16-responsive target construct, P{UAS-lacZ.B}, differs. Early expression is detected in posterior somatic cells, but not in pole cells. Expression of P{UAS-lacZ.B} in germ cells is not detected until stage 9.

Expression is observed in female germline stem cells at all stages and at a high level..

 
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
PBac{GreenEye.nosGAL4}
nos-Gal4::VP16
pBac(3xP3-EGFPafm)::nos-Gal4::(pW8-)
pBac(3xP3-EGFPafm)::nos-Gal4::(pW8-mini-white)
pBac{GreenEye.nosGal4}
Secondary FlyBase IDs
    References (6)