FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{R58E02-p65.AD}
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General Information
Symbol
P{R58E02-p65.AD}
FlyBase ID
FBtp0099550
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.R58E02
Also Known As
R58E02-p65ADZp, R58E02
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\RELAAD.R58E02
Transgenic product class
Component
Use(s)
Regulatory region(s)
GMR58E02
Encoded product / tool
p65(AD)::Zip+
Description

The R58E02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval mushroom body dopaminergic neuron i1

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196C')

(Truman et al., 2023)
larval mushroom body dopaminergic neuron k1

Comment: when combined with P{R71D01-GAL4.DBD} (combination referred to as 'MB198B')

(Truman et al., 2023)
larval mushroom body dopaminergic neuron j1

Comment: when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Truman et al., 2023)
adult stage
dopaminergic PAM neuron 1

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 10 | faint

Comment: when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 13 | intense

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 14 | intense

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 2 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 5

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 7

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

(Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 8

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

(Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 12

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 7 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 8 | faint

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 11 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 13 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 14 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 4 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 5 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 6 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 1 | faint

Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'MB313C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 12 | faint

Comment: when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 10

Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 11

Comment: when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Ichinose et al., 2015, Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 4

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 11 | intense

Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 2

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015)
dopaminergic PAM neuron 5 | intense

Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 6

Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)
dopaminergic PAM neuron 9

Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Aso et al., 2014)
dopaminergic PAM neuron 1 | intense

Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

(Aso et al., 2014)
mushroom body alpha lobe slice 1

Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Aso and Rubin, 2016)
mushroom body gamma lobe slice 5

Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

(Aso and Rubin, 2016)
adult dopaminergic PAM neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

(Lewis et al., 2015)
Stocks (16)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
58E02(DAN subset)-p65ADZp
(Zolin et al., 2021)
P{R58E02-p65.AD}
(FlyBase, 1992-)
R58E02
(Truman et al., 2023, Jovanic et al., 2019)
R58E02-p65ADZp
(Bonheur et al., 2023, Davis et al., 2020, Aso et al., 2019, Lewis et al., 2015, Yamagata et al., 2015)
Secondary FlyBase IDs
    References (41)