FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RELAAD.R58E02
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General Information
Symbol
Hsap\RELAAD.R58E02
Species
H. sapiens
Name
FlyBase ID
FBal0304522
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R58E02-p65.AD}
PBac{R58E02-p65.AD}
Also Known As
R58E02-p65ADZp, R58E02
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014)
Progenitor genotype
Carried in construct
P{R58E02-p65.AD}
PBac{R58E02-p65.AD}
Cytology
Description

The R58E02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR58E02
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval mushroom body dopaminergic neuron i1

      Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196C')

      (Truman et al., 2023)
      larval mushroom body dopaminergic neuron k1

      Comment: when combined with P{R71D01-GAL4.DBD} (combination referred to as 'MB198B')

      (Truman et al., 2023)
      larval mushroom body dopaminergic neuron j1

      Comment: when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      (Truman et al., 2023)
      adult stage
      dopaminergic PAM neuron 1

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 10 | faint

      Comment: when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

      when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 13 | intense

      Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 14 | intense

      Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 2 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 5

      Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 7

      Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

      when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

      (Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 8

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

      when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

      (Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 12

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 7 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 8 | faint

      Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

      when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 11 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 13 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 14 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 4 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 5 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 6 | faint

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 1 | faint

      Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'MB313C')

      when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 12 | faint

      Comment: when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

      (Yamagata et al., 2015)
      dopaminergic PAM neuron 10

      Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 11

      Comment: when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

      when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

      when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

      when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

      (Ichinose et al., 2015, Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 4

      Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

      when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 11 | intense

      Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

      when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 2

      Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

      (Yamagata et al., 2015)
      dopaminergic PAM neuron 5 | intense

      Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 6

      Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

      when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

      (Yamagata et al., 2015, Aso et al., 2014)
      dopaminergic PAM neuron 9

      Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

      (Aso et al., 2014)
      dopaminergic PAM neuron 1 | intense

      Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

      (Aso et al., 2014)
      mushroom body alpha lobe slice 1

      Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

      (Aso and Rubin, 2016)
      mushroom body gamma lobe slice 5

      Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

      (Aso and Rubin, 2016)
      adult dopaminergic PAM neuron | subset

      Comment: when combined with P{Ddc-GAL4.DBD}

      when combined with P{ple-GAL4.DBD}

      (Cichewicz et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R18D09

      (Lewis et al., 2015)

      abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R22E04

      (Lewis et al., 2015)

      abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R93A09

      (Lewis et al., 2015)

      abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R93G08

      (Lewis et al., 2015)

      abnormal memory | adult stage, with Nmdar1HMS02200, Scer\GAL4DBD.R37E10

      (Ichinose et al., 2015)

      abnormal memory | adult stage, with Nmdar2HMS02012, Scer\GAL4DBD.R37E10

      (Ichinose et al., 2015)

      abnormal memory | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R32D11

      (Takemura et al., 2017)

      abnormal memory | adult stage | heat sensitive, with Scer\GAL4DBD.R31F06, shits.UAS.cUa

      (Yamagata et al., 2016)

      abnormal memory | adult stage | heat sensitive, with Scer\GAL4DBD.R31F06, TrpA1UAS.(B).cKa

      (Yamagata et al., 2016)

      abnormal memory | adult stage | heat sensitive, with Scer\GAL4DBD.R37E10, shi1.UAS

      (Ichinose et al., 2015)

      abnormal memory | adult stage | heat sensitive, with Scer\GAL4DBD.R37E10, shits1.IVS.p10.20xUAS

      (Yamagata et al., 2015)

      abnormal memory | adult stage | heat sensitive, with Scer\GAL4DBD.R37E10, TrpA1UAS.(B).cKa

      (Yamagata et al., 2015)

      abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4DBD.R32D11

      (Takemura et al., 2017)

      abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1Chrimson.IVS.Syn21.10xUAS.tdTomato, Scer\GAL4DBD.R32D11

      (Takemura et al., 2017)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48H11 and Hsap\RELAAD.R58E02, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R48H11 and Hsap\RELAAD.R58E02 in adults does not affect food intake or climbing rate, compared to controls.

      (Al-Anzi and Zinn, 2018)

      Optogenetic activation (using Zzzz\ChRChrimson.IVS.Syn21.10xUAS.tdTomato or Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R32D11 and Hsap\RELAAD.R58E02 in adult females results in a strong, slow depolarization of MBON-alpha1 (mushroom body output neuron 7), sufficient to elicit a spiking response.

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R32D11 and Hsap\RELAAD.R58E02 in adult females, in combination with a paired odor, results in a short-term associative appetitive olfactory memory for this odor; preference is significantly greater when these neurons are not also activated during the testing period.

      (Takemura et al., 2017)

      Acute thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R31F06 and Hsap\RELAAD.R58E02, in combination with a paired odor, in adults results in a significant increase in short-term associative aversive olfactory memory, compared to controls.

      Inhibition (using shits.UAS.cUa at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R31F06 and Hsap\RELAAD.R58E02 in adults significantly reduces short-term associative appetitive, but not aversive, olfactory memory, compared to controls, with no effect on sugar preference.

      (Yamagata et al., 2016)

      Inhibition (using shi1.UAS at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R37E10 and Hsap\RELAAD.R58E02 in adults during, or immediately after, training, but not during, or immediately before, testing, significantly impairs long-term appetitive olfactory memory, compared to controls; inhibition does not affect short-term memory.

      (Ichinose et al., 2015)

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R93G08 and Hsap\RELAAD.R58E02, the split-GAL4 pair of Scer\GAL4DBD.R18D09 and Hsap\RELAAD.R58E02, or the split-GAL4 pair of Scer\GAL4DBD.R22E04 and Hsap\RELAAD.R58E02, in adults results in a significant attraction to the illuminated area; activation of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R93A09 and Hsap\RELAAD.R58E02 results in a non-significant attraction to the illuminated area, no attraction is observed in controls.

      (Lewis et al., 2015)

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R37E10 and Hsap\RELAAD.R58E02 in adults, in combination with a paired odor, results in a significant increase in long-term associative attractive olfactory memory, compared to controls.

      Inhibition (using shits1.IVS.p10.20xUAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R37E10 and Hsap\RELAAD.R58E02 in adults during training significantly reduces long-term, but not short-term, associative appetitive (sugar) olfactory memory, compared to controls.

      (Yamagata et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (23)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)

      FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

      (Lewis et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R58E02.T:Zzzz\Zip+
      Hsap\RELAAD.R58E02
      Name Synonyms
      Secondary FlyBase IDs
        References (46)