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General Information
Symbol
Scer\GAL4DBD.R32D11
Species
S. cerevisiae
Name
FlyBase ID
FBal0304452
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R32D11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
dopaminergic PAM neuron 10 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

dopaminergic PAM neuron 11 | intense

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

dopaminergic PAM neuron 13 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

dopaminergic PAM neuron 14 | faint

Comment: when combined with P{R58E02-p65.AD} (combination referred to as 'MB043B')

when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

mushroom body alpha lobe slice 1

Comment: when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

dopaminergic PAM neuron 11

Comment: when combined with PBac{R58E02-p65.AD} (combination referred to as 'MB043C')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Optogenetic activation (using Zzzz\ChRChrimson.IVS.Syn21.10xUAS.tdTomato or Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R32D11 and Hsap\RELAAD.R58E02 in adult females results in a strong, slow depolarization of MBON-alpha1 (mushroom body output neuron 7), sufficient to elicit a spiking response.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R32D11 and Hsap\RELAAD.R58E02 in adult females, in combination with a paired odor, results in a short-term associative appetitive olfactory memory for this odor; preference is significantly greater when these neurons are not also activated during the testing period.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R32D11.T:Zzzz\Zip-
Scer\GAL4DBD.R32D11
Name Synonyms
Secondary FlyBase IDs
    References (7)