FlyBase Recombinant Construct Report: PBac{R58E02-p65.AD}

General Information

Symbol

PBac{R58E02-p65.AD}

FlyBase ID

FBtp0099551

Feature type

transgenic_transposable_element

Component Allele(s)

Hsap\RELA^AD.R58E02

Also Known As

R58E02-p65ADZp, R58E02

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Hsap\RELA^AD.R58E02

Transgenic product class

Component

Use(s)

Regulatory region(s)

GMR58E02

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The R58E02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Hsap\RELA^AD.R58E02

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron i1

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196C')

(Truman et al., 2023)

larval mushroom body dopaminergic neuron k1

Comment: when combined with P{R71D01-GAL4.DBD} (combination referred to as 'MB198B')

(Truman et al., 2023)

larval mushroom body dopaminergic neuron j1

Comment: when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Truman et al., 2023)

adult stage

dopaminergic PAM neuron 1

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 10 | faint

Comment: when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 13 | intense

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 14 | intense

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 2 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 5

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 7

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

(Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 8

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312C')

when combined with P{R10G03-GAL4.DBD} (combination referred to as 'MB312B')

(Aso and Rubin, 2016, Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 12

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 7 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 8 | faint

Comment: when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 11 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 13 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 14 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 4 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 5 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 6 | faint

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 1 | faint

Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'MB313C')

when combined with P{R22E04-GAL4.DBD} (combination referred to as 'MB042B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 12 | faint

Comment: when combined with P{R31F06-GAL4.DBD} (combination referred to as 'MB195B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 10

Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 11

Comment: when combined with P{R37E10-GAL4.DBD} (combination referred to as 'MB299B')

when combined with P{R11A03-GAL4.DBD} (combination referred to as 'MB188B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Ichinose et al., 2015, Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 4

Comment: when combined with P{R18D09-GAL4.DBD} (combination referred to as 'MB040B')

when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 11 | intense

Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043B')

when combined with P{R87B09-GAL4.DBD} (combination referred to as 'MB045B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 2

Comment: when combined with P{R36B06-GAL4.DBD} (combination referred to as 'MB196B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 5 | intense

Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316C')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 6

Comment: when combined with P{R93A09-GAL4.DBD} (combination referred to as 'MB047B')

when combined with P{R93G08-GAL4.DBD} (combination referred to as 'MB316B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 9

Comment: when combined with P{R29G04-GAL4.DBD} (combination referred to as 'MB194B')

(Aso et al., 2014)

dopaminergic PAM neuron 1 | intense

Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

(Aso et al., 2014)

mushroom body alpha lobe slice 1

Comment: when combined with P{R32D11-GAL4.DBD} (combination referred to as 'MB043C')

(Aso and Rubin, 2016)

mushroom body gamma lobe slice 5

Comment: when combined with P{R48H11-GAL4.DBD} (combination referred to as 'MB315C')

(Aso and Rubin, 2016)

adult dopaminergic PAM neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Images

Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (7)

Stocks containing insertion(s) of this recombinant construct

Bloomington

68252

w¹¹¹⁸; P{R10G03-GAL4.DBD}attP2 PBac{R58E02-p65.AD}VK00027

68315

w¹¹¹⁸; P{R17C11-GAL4.DBD}attP2 PBac{R58E02-p65.AD}VK00027

68316

w¹¹¹⁸; P{R48H11-GAL4.DBD}attP2 PBac{R58E02-p65.AD}VK00027

68363

w¹¹¹⁸; P{R32D11-GAL4.DBD}attP2 PBac{R58E02-p65.AD}VK00027

603440

w¹¹¹⁸; PBac{R58E02-p65.AD}VK00027, P{R36B06-GAL4.DBD}attP2

600631

w¹¹¹⁸; Kr^If-1/CyO, P{2xTb¹-RFP}CyO; PBac{R58E02-p65.AD}VK00027

603441

w¹¹¹⁸; PBac{R58E02-p65.AD}VK00027/CyO, P{2xTb¹-RFP}CyO; P{R32G08-GAL4.DBD}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PBac{R58E02-p65.AD}

(FlyBase, 1992-)

R58E02

(Truman et al., 2023, Jovanic et al., 2019)

R58E02-p65ADZp

(Davis et al., 2020, Takemura et al., 2017, Yamagata et al., 2015)

Secondary FlyBase IDs

References (26)

Report Sections

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