FlyBase Recombinant Construct Report: P{R59E08-p65.AD}

General Information

Symbol

P{R59E08-p65.AD}

FlyBase ID

FBtp0118967

Feature type

transgenic_transposable_element

Component Allele(s)

Hsap\RELA^AD.R59E08

Also Known As

R59E08-AD, R59E08-p65ADZp

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Hsap\RELA^AD.R59E08

Transgenic product class

Component

Use(s)

Regulatory region(s)

GMR59E08

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Description

The GMR59E08 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Hsap\RELA^AD.R59E08

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

T4 neuron

Comment: when combined with P{R42F06-GAL4.DBD}

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'SS00324')

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'T4/T5')

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'T4/T5-SplitGal4')

when combined with P{R59E08-GAL4.DBD}

(Tanaka and Clark, 2022, Drews et al., 2020, Hörmann et al., 2020, Keleş et al., 2020, Klapoetke et al., 2017, Strother et al., 2017, Bahl et al., 2015)

T5 neuron

Comment: when combined with P{R42F06-GAL4.DBD}

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'SS00324')

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'T4/T5')

when combined with P{R42F06-GAL4.DBD} (combination referred to as 'T4/T5-SplitGal4')

when combined with P{R59E08-GAL4.DBD}

(Tanaka and Clark, 2022, Drews et al., 2020, Hörmann et al., 2020, Keleş et al., 2020, Klapoetke et al., 2017, Strother et al., 2017, Bahl et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

71101

w¹¹¹⁸; P{R59E08-p65.AD}attP40

86858

w¹¹¹⁸; P{R59E08-p65.AD}attP40; P{R42F06-GAL4.DBD}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

59E08-p65ADZp

(Longden et al., 2023)

P{R59E08-p65.AD}

(Shen et al., 2025, FlyBase, 1996-)

R59E08-AD

(Drews et al., 2020, Hörmann et al., 2020, Keleş et al., 2020, Matulis et al., 2020, Tanaka and Clark, 2020, Creamer et al., 2018, Mauss et al., 2017, Leonhardt et al., 2016, Pankova and Borst, 2016, Bahl et al., 2015, Schilling and Borst, 2015)

R59E08-p65.AD

(Keleş et al., 2020)

R59E08-p65ADZp

(Keleş et al., 2019, Klapoetke et al., 2017, Strother et al., 2017)

R59E08.p65ADZp

(Ribeiro et al., 2018)

R59E08AD

(Tanaka and Clark, 2022)

Secondary FlyBase IDs

References (27)

Report Sections

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