FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{twy.GFP}
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General Information
Symbol
P{twy.GFP}
FlyBase ID
FBtp0142653
Feature type
transgenic_transposable_element
Component Allele(s)
twyGFP
Also Known As
FBF1-GFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele twyGFP
Transgenic product class
genomic_DNA
(Hou et al., 2020)
wild_type
(Hou et al., 2020)
Component
Use(s)
Regulatory region(s)
twy
Encoded product / tool
twy
Tagged with
GFP
Description

The coding and upstream regulatory sequences of CG5964 (amplified from genomic DNA) are cloned in frame with a downstream GFP tag.

(Hou et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
pupal stage
scolopidial cilium
(Hou et al., 2023)
antennal segment 3 sense organ
(Hou et al., 2023)
Johnston organ
(Hou et al., 2023)
adult stage
scolopidial cilium
(Hou et al., 2023)
antennal segment 3 sense organ
(Hou et al., 2023)
Johnston organ
(Hou et al., 2023)
adult stage | male
spermatocyte | restricted
(Hou et al., 2023)
spermatid | restricted
(Hou et al., 2023)
Additional Information
Statement
Reference

twy protein localizes at the ciliary base in both ciliated chordotonal organs and spermatocytes. In spermatocytes, it localizes to centrioles just below the transition zone and at the base of both spermatocyte primary-like cilia and spermatid ciliary caps, forming ringlike structures surrounding microtubule doublets.

(Hou et al., 2023)
 
Marker for
Reflects expression of
twy
(Hou et al., 2023)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
CG5964-GFP
(Hou et al., 2020)
FBF1-GFP
(Wu et al., 2020)
Fbf1-GFP
(Hou et al., 2023)
P{CG5964.GFP}
(FlyBase, 1992-)
P{twy.GFP}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (6)