FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{foxo-dCas9::VPR,pyr.gRNAx2}
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General Information
Symbol
PBac{foxo-dCas9::VPR,pyr.gRNAx2}
FlyBase ID
FBtp0144909
Feature type
transgenic_transposable_element
Component Allele(s)
pyrgRNAx2
Spyo\Cas9dCas9::VPR.foxo
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele pyrgRNAx2
Transgenic product class
sgRNA
(Maselko et al., 2020)
Component
Use(s)
Encoded product / tool
pyr
Description

Expresses two sgRNAs (the same as in P{TOE.GS00085}), both of which target the pyr promoter.

(Maselko et al., 2020)
Component allele Spyo\Cas9dCas9::VPR.foxo
Transgenic product class
Component
Use(s)
Regulatory region(s)
foxo
Encoded product / tool
dCas9::VPR
Description

The foxo promoter drives expression of the dCas9::VPR transcriptional activator.

(Maselko et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{foxo-dCas9::VPR,pyr.gRNAx2}
(FlyBase, 1996-)
Pfoxo::PTA::pyr sgRNA
(Maselko et al., 2020)
Secondary FlyBase IDs
    References (3)