A tandem array containing 12 copies of a mutated histone gene repeat unit; in each repeat, the 3'UTR of the His3 gene has been replaced by the wild-type 3'UTR sequence of the orthologous His3 gene from D. simulans.
Wild-type His1 gene in the context of a histone gene repeat tandem array. A number of synonymous mutations have been introduced (resulting in the addition and removal of restriction enzyme sites) so that the transgenic gene can be distinguished molecularly from the endogenous gene copies.
Wild-type His2A gene in the context of a histone gene repeat tandem array. A number of synonymous mutations have been introduced (resulting in the addition and removal of restriction enzyme sites) so that the transgenic gene can be distinguished molecularly from the endogenous gene copies.
Wild-type His2B gene in the context of a histone gene repeat tandem array. A number of synonymous mutations have been introduced (resulting in the addition of restriction enzyme sites) so that the transgenic gene can be distinguished molecularly from the endogenous gene copies.
Wild-type His4 gene in the context of a histone gene repeat tandem array. A number of synonymous mutations have been introduced (resulting in the addition and removal of restriction enzyme sites) so that the transgenic gene can be distinguished molecularly from the endogenous gene copies.
