Allele Dmel\egh⁷

General Information
Symbol	Dmel\egh⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0006837
Feature type	allele	Associated gene	Dmel\egh

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	X ray, ethylenimine, ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Soller et al., 2006)
Mutagen	ethylenimine ethyl methanesulfonate (Goode et al., 1996) X ray
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation X:2,491,481..2,491,481 comment=Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T2491481A pr_change=M308K\|egh-PC,M308K\|egh-PB reported_pr_change=M308K (Wandall et al., 2005)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The A to T transversion results in the M308K substitution. T347C and T409C are point mutations in the UTR of exon 2 and there is also a C added at position 278. (Soller et al., 2006) Nucleotide substitution: A?T. (Soller et al., 2006) Nucleotide substitution: T347C. (Soller et al., 2006) Nucleotide substitution: T409C. (Soller et al., 2006) Amino acid replacement: M308K. (Wandall et al., 2005, Soller et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (with egh^cm) (Soller et al., 2006) female sterile (Goode et al., 1996) lethal (Soller et al., 2006) lethal \| pupal stage \| recessive (Goode et al., 1996) lethal \| recessive (Williams et al., 1995, Judd et al., 1972) neuroanatomy defective (Fan et al., 2005)
Phenotype Manifest In
	basal stalk \| germline clone (Goode et al., 1996) dorsal appendage \| germline clone (Goode et al., 1996) embryonic/larval optic neuropil (Fan et al., 2005) follicle cell \| germline clone (Goode et al., 1996) lamina anlage (Fan et al., 2005) lamina anlage glial cell (Fan et al., 2005) lamina plexus (Fan et al., 2005) lobula (Fan et al., 2005) lobula & neuron (Fan et al., 2005) nurse cell \| germline clone (Goode et al., 1996) oocyte \| germline clone (Goode et al., 1996) photoreceptor cell R1 & axon (Fan et al., 2005) photoreceptor cell R2 & axon (Fan et al., 2005) photoreceptor cell R3 & axon (Fan et al., 2005) photoreceptor cell R4 & axon (Fan et al., 2005) photoreceptor cell R5 & axon (Fan et al., 2005) photoreceptor cell R6 & axon (Fan et al., 2005) presumptive embryonic/larval nervous system \| germline clone (Goode et al., 1996) ventral nerve cord (with egh^cm) (Soller et al., 2006) ventral nerve cord \| somatic clone (Soller et al., 2006)
Detailed Description
	Statement Reference egh^cm/egh⁷ females are insensitive to injection with sex peptide; oviposition is not induced in these females and mating is not suppressed. Remating in egh^cm and egh^cm/egh⁷ females is about 48% and 24% compared to mating of virgin egh^cm and egh^cm/egh⁷ females, which is a higher amount than in control females. Sex appeal of mated egh^cm/egh⁷ females to males is reduced to the same extent as control mated females. egh⁷ clones in the VNC Ap neurons do not extend neuronal projections to the anterior part of the central brain and fail to form the elaborate arborizations that are observed in wild-type flies. In larval brains of egh^cm/egh⁷ mutants, Ap neurons projecting from the VNC to the CNS show pathfinding errors and fail to reach the target area. In many cases, these axons defasciculate and turn backward. Mostly, these axons stop extending toward the central brain but rarely defasciculate. (Soller et al., 2006) In the brains of egh⁷ third instar larvae, some of the axons of R-cells 1-6 fail to terminate in the lamina and project into the medulla, causing thick axon bundles in this structure. R1-R6 axons that do terminate in the lamina are disorganized and form abnormal patches in the lamina plexus. In egh⁷ mutants, the boundary region that exists in wild type between the lobula and lamina is no longer apparent and the sheath-like glial processes that usually delimit this boundary are severely disrupted or missing altogether. This allows lobula distal neurons to cross into the base of the developing lamina, disrupt the pattern of lamina glia and result in the perturbed R1-R6 axonal projections. Clonal analysis shows that the egh⁷ is required in cells of the lobula complex primordium, as egh⁷ mutant clones in this structure are associated the same perturbed R1-R6 axonal projection phenotype as egh⁷ flies. However, egh⁷ clones in the eye, glial cells, lamina precursors, or lamina neurons do not cause this phenotype. (Fan et al., 2005) Females with germline clones lay eggs with fused dorsal appendages and the embryos have an expanded nervous system. Only a few eggs are laid because early oogenesis is blocked due to the development of egg chambers containing multiple oocyte-nurse cell complexes. Germ cell divisions in the egg chambers are not regulated. Follicle cells at the posterior of egg chamber become mesenchymal-like indicating they have not adopted a border cell fate. Stalk cells are determined correctly but are usually disorganised compared to wild type. (Goode et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement Reference egh⁷ has neuroanatomy defective phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has neuroanatomy defective phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has neuroanatomy defective phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005)
Suppressed by
Statement Reference egh⁷ has neuroanatomy defective phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005)
NOT suppressed by
Statement Reference egh⁷ has neuroanatomy defective phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has neuroanatomy defective phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has neuroanatomy defective phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005)
NOT Enhancer of
Statement Reference egh⁷ is a non-enhancer of visible phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PU (Protzer et al., 2009)
NOT Suppressor of
Statement Reference egh⁷ is a non-suppressor of visible phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Other
Statement Reference egh⁷, h[+]/h²⁶ has lethal \| partially phenotype (Bianchi-Frias et al., 2004) egh⁷, h²⁶ has lethal \| partially phenotype (Bianchi-Frias et al., 2004, Bianchi-Frias et al., 2004) egh⁷, hⁱ²²/h[+] has lethal \| partially phenotype (Bianchi-Frias et al., 2004) egh⁷, hⁱ²² has lethal \| partially phenotype (Bianchi-Frias et al., 2004, Bianchi-Frias et al., 2004)
Phenotype Manifest In
NOT Enhanced by
Statement Reference egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by sli²/sli[+] (Fan et al., 2005)
Suppressed by
Statement Reference egh⁷ has photoreceptor cell R1 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, suppressible by Hsap\B4GALT6^Scer\UAS.cWa/Scer\GAL4^arm.PS (Fan et al., 2005)
NOT suppressed by
Statement Reference egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo^{dsRNA.Scer\UAS} (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by sli[+]/sli^k04807b (Fan et al., 2005) egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by sli²/sli[+] (Fan et al., 2005)
NOT Enhancer of
Statement Reference egh⁷ is a non-enhancer of eye phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PU (Protzer et al., 2009)
NOT Suppressor of
Statement Reference egh⁷ is a non-suppressor of eye phenotype of H^Scer\UAS.cMa/H^Scer\UAS.cMa, Scer\GAL4^GMR.PU (Protzer et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference The eye phenotype caused by expression of H[Scer\UAS.cMa] under the control of Scer\GAL4[GMR.PU] is unaffected if the flies also carry egh[7]. (Protzer et al., 2009) In egh⁷/Y; sli²/+ and egh⁷/Y; sli^k04807b/+ mutants, the R-cell axonal projection pattern is defective like in egh⁷ single mutants. When egh⁷ is heterozygous in these double mutants, flies appear wild type. Expression of robo^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^elav-C155, does not alter the R-cell phenotype of egh⁷ mutants. (Fan et al., 2005)
Xenogenetic Interactions
Statement Reference Expression of Hsap\β4Gal-T6^Scer\UAS.cWa, under the control of Scer\GAL4^arm.PS, in egh⁷ mutants rescues the R-cell axonal projection phenotype. (Fan et al., 2005)
Complementation & Rescue Data
Rescued by	egh⁷/egh^cm is rescued by egh^+tP2 (Soller et al., 2006) egh⁷/egh^cm is rescued by Scer\GAL4^elav-C155/egh^{Scer\UAS.T:Ivir\HA1} (Soller et al., 2006) egh⁷ is rescued by egh^+t7 (Goode et al., 1996, Williams et al., 1995) egh⁷ is rescued by egh^+tP2 (Soller et al., 2006) egh⁷ is rescued by egh^hs.PG (Goode et al., 1996) egh⁷ is rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^tub.PU (Soller et al., 2006) egh⁷ is rescued by Scer\GAL4^elav-C155/egh^{Scer\UAS.T:Ivir\HA1} (Soller et al., 2006) egh⁷ is rescued by Scer\GAL4^αTub84B.PL/egh^Scer\UAS.cFa (Fan et al., 2005)
Partially rescued by	egh⁷/egh^cm is partially rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ap-md544 (Soller et al., 2006) egh⁷/egh^cm is partially rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^tub.PU (Soller et al., 2006) egh⁷/egh^cm is partially rescued by Scer\GAL4^tsh-md621/egh^{Scer\UAS.T:Ivir\HA1} (Soller et al., 2006) egh⁷ is partially rescued by egh^P1 (Soller et al., 2006) egh⁷ is partially rescued by Scer\GAL4^elav-C155/egh^Scer\UAS.cFa (Fan et al., 2005) egh⁷ is partially rescued by Scer\GAL4^tsh-md621/egh^{Scer\UAS.T:Ivir\HA1} (Soller et al., 2006)
Not rescued by	egh⁷/egh^cm is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav.PLu (Soller et al., 2006) egh⁷/egh^cm is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^gcm-rA87.C/Scer\GAL4^gcm-rA87.C (Soller et al., 2006) egh⁷/egh^cm is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^repo (Soller et al., 2006) egh⁷ is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^ap-md544 (Soller et al., 2006) egh⁷ is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^elav.PLu (Soller et al., 2006) egh⁷ is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^gcm-rA87.C/Scer\GAL4^gcm-rA87.C (Soller et al., 2006) egh⁷ is not rescued by egh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^repo (Soller et al., 2006) egh⁷ is not rescued by Scer\GAL4^1.3D2/egh^Scer\UAS.cFa (Fan et al., 2005) egh⁷ is not rescued by Scer\GAL4^gcm.PS/egh^Scer\UAS.cFa (Fan et al., 2005) egh⁷ is not rescued by Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF/egh^Scer\UAS.cFa (Fan et al., 2005)
Comments	Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^ap-md544 or Scer\GAL4^tsh-md621 rescues the egg retention phenotype of egh^cm/egh⁷ females. Most egh^cm/egh⁷ larval brains show a complete rescue of the ventral nerve cord defect when egh^{Scer\UAS.T:Ivir\HA1} in expressed under the control of Scer\GAL4^ap-md544. Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tsh-md621 rescues the viability of egh⁷ flies but these flies are short lived. Scer\GAL4^ap-md544>egh^{Scer\UAS.T:Ivir\HA1} expression does not rescue egh⁷ viability. Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tsh-md621 rescues the receptivity response of egh^cm/egh⁷ females to sex peptide completely. This response is partially rescued when egh^{Scer\UAS.T:Ivir\HA1} is expressed under the control of either Scer\GAL4^tub or Scer\GAL4^ap-md544, but is not rescued when Scer\GAL4^elav.PLu is used to drive expression. Oviposition after treatment with sex peptide is rescued in 67% of egh^cm/egh⁷ females (the other 33% lay no eggs) when egh^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ap-md544 and is rescued to wild-type levels when driven by either Scer\GAL4^tub or Scer\GAL4^tsh-md621. Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu does not rescue oviposition. (Soller et al., 2006) Expression of egh^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 rescues the R-cell projection phenotype in 80% of mutant egh⁷ brain hemispheres tested. (Fan et al., 2005) Lethality and fertility phenotypes are rescued by egh^+t7 and egh^hs.PG. (Goode et al., 1996) Lethality is rescued by egh^+t7, rescued adults are fertile. (Williams et al., 1995)
Stocks ( 2 )
Bloomington	3902 egh⁷/FM7a/Dp(1;2;Y)w⁺
Kyoto	107261 egh⁷/FM7a/Dp(1;2;Y)w⁺
Notes on Origin
Discoverer	Alexander.

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	egh⁷ (Soller et al., 2006, Protzer et al., 2009, Popodi et al., 2010-) egh^65h5 (Goode et al., 1996) egh^65h6 (Williams et al., 1995) l(1)3Ae⁷ l(1)zw4^h6 (Judd et al., 1972, )
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Protzer et al., 2009, Cell Tissue Res. 336(1): 149--158 Drosophila alpha-1,4-glycosyltransferase (alpha-4GT1) inhibits reaper-mediated apoptosis in the eye. [FBrf0215427] Soller et al., 2006, Curr. Biol. 16(18): 1771--1782 Sex-peptide-regulated female sexual behavior requires a subset of ascending ventral nerve cord neurons. [FBrf0192935] Fan et al., 2005, Dev. Biol. 287(1): 61--73 The egghead gene is required for compartmentalization in Drosophila optic lobe development. [FBrf0190118] Wandall et al., 2005, J. Biol. Chem. 280(6): 4858--4863 Egghead and brainiac are essential for glycosphingolipid biosynthesis in vivo. [FBrf0184049] Bianchi-Frias et al., 2004, PLoS Biol. 2(7): e178 Hairy transcriptional repression targets and cofactor recruitment in Drosophila. [FBrf0179136] Goode et al., 1996, Development 122(12): 3863--3879 The neurogenic genes egghead and brainiac define a novel signaling pathway essential for epithelial morphogenesis during Drosophila oogenesis. [FBrf0091297] Williams et al., 1995, J. Cell Biol. 129(3): 709--723 The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis. [FBrf0082780] Judd et al., 1972, Genetics 71: 139--156 The anatomy and function of a segment of the X chromosome of Drosophila melanogaster. [FBrf0023906]
Personal communication to FlyBase	Popodi et al., 2010-, Small X duplications for the stock center collection. Small X duplications for the stock center collection. [FBrf0210621]

Allele Dmel\egh7

Allele Dmel\egh⁷