FlyBase Allele Report: Dmel\Not3[KK102144]

General Information

Symbol

Dmel\Not3^KK102144

Species

D. melanogaster

Name

FlyBase ID

FBal0231638

Feature type

allele

Associated gene

Dmel\Not3

Associated Insertion(s)

Carried in Construct

P{KK102144}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102144}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102144

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Delta^UAS.cDa

(Vicente et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Delta^UAS.cDa

(De Keersmaecker et al., 2013)

modeled by Ras85D^V12.cUa.UAS

(Vicente et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

decreased cell number | larval stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

decreased occurrence of cell division | larval stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

increased cell death | larval stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

visible | adult stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

eye disc, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

photoreceptor precursor cell | larval stage, with Scer\GAL4^ey.PH

(Vicente et al., 2018)

Detailed Description

Statement

Reference

The expression of Not3^KK102144 under the control of Scer\GAL4^ey.PH induces a fully penetrant small rough eye phenotype. The eye disc is mishappen, exhibits a significant decrease in cell divisions (BrdU labelling and pH3 immunostaining) and a significant increase in apoptosis (Dcp1a immunostaining), as compared to controls; differentiation is strongly inhibited, although some photoreceptors still form.

(Vicente et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH has neoplasia | adult stage phenotype, suppressible by Fancl^GD7835, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Delta^UAS.cDa, Not3^KK102144, Scer\GAL4^ey.PH has neoplasia | adult stage phenotype, suppressible by CycB²