FlyBase Allele Report: Dmel\Tim8[JF01533]

General Information

Symbol

Dmel\Tim8^JF01533

Species

D. melanogaster

Name

FlyBase ID

FBal0245792

Feature type

allele

Associated gene

Dmel\Tim8

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01533}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

(Ni et al., 2008)

Progenitor genotype

Carried in construct

P{TRiP.JF01533}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Ni et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01533

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Phenotype Manifest In

dopaminergic PPL1 neuron | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Detailed Description

Statement

Reference

Expression of Tim8^JF01533 under the control of Scer\GAL4^ple.PF results in a loss of dopaminergic (DA) neurons from the PPL1 cluster accompanied by mitochondrial aggregations in the DA neurons in the adult brain but expression under the Scer\GAL4^Mhc.PU driver has no adverse effect on adult wing posture or jumping ability.

(Gehrke et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ple.PF, Tim8^JF01533 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Scer\GAL4^ple.PF, Tim8^JF01533 has decreased cell number | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Enhancer of

Statement

Reference

Tim8^JF01533/Scer\GAL4^Mhc.PU is an enhancer of visible | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tim8^JF01533/Scer\GAL4^Mhc.PU is an enhancer of abnormal jumping phenotype of Pink1^B9

(Gehrke et al., 2015)

Tim8^JF01533, Scer\GAL4^Mhc.PU is an enhancer of visible | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Tim8^JF01533, Scer\GAL4^Mhc.PU is an enhancer of abnormal jumping phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Tim8^JF01533 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Tim8^JF01533 is a non-suppressor of decreased cell number | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ple.PF, Tim8^JF01533 has dopaminergic PPL1 neuron | adult stage phenotype, non-suppressible by Pink1^UAS.cYa, Scer\GAL4^ple.PF

(Gehrke et al., 2015)

Enhancer of

Statement

Reference

Tim8^JF01533, Scer\GAL4^Mhc.PU is an enhancer of flight muscle cell phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Tim8^JF01533, Scer\GAL4^Mhc.PU is an enhancer of mitochondrion | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

Tim8^JF01533/Scer\GAL4^Mhc.PU is an enhancer of wing | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tim8^JF01533/Scer\GAL4^Mhc.PU is an enhancer of flight muscle cell phenotype of Pink1^B9

(Gehrke et al., 2015)

Tim8^JF01533/Scer\GAL4^Mhc.PU is an enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Tim8^JF01533 is an enhancer of dopaminergic neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Scer\GAL4^ple.PF/Tim8^JF01533 is an enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Tim8^JF01533, Scer\GAL4^Mhc.PU is an enhancer of wing | adult stage phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PU

(Gehrke et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ple.PF/Tim8^JF01533 is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Gehrke et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of dopaminergic PPL1 neurons observed in adult flies expressing Tim8^JF01533 RNAi under the control of Scer\GAL4^ple.PF is not affected by co-expression of Pink1^Scer\UAS.cYa.

The abnormal mitochondrial aggregations (observed in flight muscles or dopaminergic neurons), wing posture and jumping ability defects as well as the loss of dopaminergic neurons from the PPL1 cluster characteristic for Pink1^B9 adult homozygotes are exacerbated (or at least not suppressed) by expression (controlled by either the Scer\GAL4^Mhc.PU muscle driver or the Scer\GAL4^ple.PF neuronal driver) of Tim8^JF01533. Similarly, the mitochondrial aggregations in flight muscles along with the wing posture and jumping ability defects observed in adult flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PU are further enhanced by expression of Tim8^JF01533.

(Gehrke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31082

y¹ v¹; P{TRiP.JF01533}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tim8^JF01533

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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