Reference Report

Reference
Citation	Baumann, A., Barry, J., Wang, S., Fujiwara, Y., Wilson, T.G. (2010). Paralogous Genes Involved in Juvenile Hormone Action in Drosophila melanogaster. Genetics 185(4): 1327--1336. (Export to RIS)
FlyBase ID	FBrf0211496
Publication Type	Research paper
PubMed ID	20498297
PubMed Abstract	Juvenile hormone (JH) is critical for multiple aspects of insect development and physiology. Although roles for the hormone have received considerable study, an understanding of the molecules necessary for JH action in insects has been frustratingly slow to evolve. Methoprene-tolerant (Met) in Drosophila melanogaster fulfills many of the requirements for a hormone receptor gene. A paralogous gene, germ-cell expressed (gce), possesses homology and is a candidate as a Met partner in JH action. Expression of gce was found to occur at multiple times and in multiple tissues during development, similar to that previously found for Met. To probe roles of this gene in JH action, we carried out in vivo gce over- and underexpression studies. We show by overexpression studies that gce can substitute in vivo for Met, alleviating preadult but not adult phenotypic characters. We also demonstrate that RNA interference-driven knockdown of gce expression in transgenic flies results in preadult lethality in the absence of MET. These results show that (1) unlike Met, gce is a vital gene and shows functional flexibility and (2) both gene products appear to promote JH action in preadult but not adult development.
DOI	10.1534/genetics.110.116962
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (9)
	gce^{dsRNA.Scer\UAS.cBa} gce^Scer\UAS.cBa Met²⁷ Met^W3 Scer\GAL4^Act5C.PI Scer\GAL4^dan-AC116 Scer\GAL4^Lsp2.PH Scer\GAL4^tub.PU w^+mC
Constructs (5)
	P{Act5C-GAL4} P{Lsp2-GAL4.H} P{tub-GAL4.U} P{UAS-gce.B} P{UAS-gce.RNAi}
Genes (4)
	gce Met Scer\GAL4 w
Insertions (1)
	P{GawB}dan^AC116
Natural transposons (1)
	P-element