FlyBase Allele Report: Dmel\dsh[4]

General Information

Symbol

Dmel\dsh⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0003141

Feature type

allele

Associated gene

Dmel\dsh

Associated Insertion(s)

Carried in Construct

Also Known As

dsh^VA153

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Perrimon and Mahowald, 1987)

loss of function allele

(Lecuit and Cohen, 1997, Neumann and Cohen, 1997)

Mutagen

ethyl methanesulfonate

(Perrimon and Mahowald, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | embryonic stage

(Perrimon and Mahowald, 1987)

abnormal neuroanatomy

(Sato et al., 2006)

lethal | recessive

(Theisen et al., 1994)

lethal - all die before end of larval stage | recessive | rescuable maternal effect

(Perrimon and Mahowald, 1987)

some die during larval stage | rescuable maternal effect

(Perrimon and Mahowald, 1987)

visible, with dsh^w

(Haerry et al., 1997)

Phenotype Manifest In

adult, with dsh^w

(Haerry et al., 1997)

antenna, with dsh^w

(Haerry et al., 1997)

egg chamber (with dsh¹)

(Cohen et al., 2002)

embryonic/first instar larval cuticle | dorsal | germline clone

(Perrimon and Mahowald, 1987)

embryonic/larval spiracle primordium | germline clone | posterior

(Perrimon and Mahowald, 1987)

eye photoreceptor cell

(Sato et al., 2006)

filzkorper | germline clone

(Perrimon and Mahowald, 1987)

follicle stem cell | somatic clone

(Song and Xie, 2003)

germarium (with dsh¹)

(Cohen et al., 2002)

lamina

(Sato et al., 2006)

neuron

(Sato et al., 2006)

ovarian sheath (with dsh¹)

(Cohen et al., 2002)

ovary (with dsh¹)

(Cohen et al., 2002)

scutum, with dsh^w

(Haerry et al., 1997)

wing, with dsh^w

(Haerry et al., 1997)

Detailed Description

Statement

Reference

dsh⁴ mutants exhibit a ventral-to-dorsal misrouting phenotype.

Induction of dsh⁴ homozygous clones in the retina does not result in R axon misrouting, whereas when the whole retina is dsh⁴ mutant, axonal misrouting is found.

(Sato et al., 2006)

Mutant somatic stem cell (SSC) clones in the ovary are lost more rapidly than wild-type clones; 92% and 100% of the marked dsh⁴ SSC clones observed one week after clone induction are lost two or three weeks after clone induction respectively. dsh⁴ follicle cell clones are normal in size and the mutant follicle cells have normal morphology.

(Song and Xie, 2003)

dsh¹/dsh⁴ heterozygotes show an ovary phenotype like that of Wnt4 mutants, though with reduced penetrance, with germaria flopped over the older egg chambers due to indequate ovary sheath.

(Cohen et al., 2002)

5% of dsh⁴/Y flies carrying one copy of dsh^w have defects in adult structures. The defects consist primarily of deleted or duplicated antennae and/or deleted wings with a concurrent duplication of notal structures.

(Haerry et al., 1997)

Clonal analysis revealed polarity defects in adult tissues as for dsh¹, and that dsh is required autonomously for cell polarity. Similar mutant leg phenotypes, e.g. duplications and bifurcations, are produced by dsh clones, dsh, wg double heterozygotes and wg mutants. There is allele specificity in these interactions.

(Theisen et al., 1994)

Females possessing homozygous germline clones when mated to wild type males produce two classes of embryos: heterozygous females are fertile and morphogenetically normal and hemizygous male embryos lack dorsal cuticle, posterior spiracles and filzkorper material. The ventral cuticle present has no naked cuticle in the thoracic or abdominal segments. Embryos show an aberrant pattern of cell death and lack of parasegmental and segmental boundaries.

(Perrimon and Mahowald, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dsh⁴ has abnormal neuroanatomy phenotype, enhanceable by hid^GMR.PU

(Sato et al., 2006)

dsh⁴, dsh^w has visible phenotype, enhanceable by wg[+]/wg^en11

(Haerry et al., 1997)

dsh⁴, dsh^w has visible phenotype, enhanceable by sgl[+]/sgl^SG9

(Haerry et al., 1997)

dsh⁴, dsh^w has visible phenotype, enhanceable by sgl[+]/sgl⁰⁸³¹⁰

(Haerry et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

dsh⁴ has lamina phenotype, enhanceable by hid^GMR.PU

(Sato et al., 2006)

dsh⁴ has eye photoreceptor cell phenotype, enhanceable by hid^GMR.PU

(Sato et al., 2006)

dsh⁴ has neuron phenotype, enhanceable by hid^GMR.PU

(Sato et al., 2006)

dsh⁴, dsh^w has adult phenotype, enhanceable by wg[+]/wg^en11

(Haerry et al., 1997)

dsh⁴, dsh^w has adult phenotype, enhanceable by sgl[+]/sgl^SG9

(Haerry et al., 1997)

dsh⁴, dsh^w has adult phenotype, enhanceable by sgl[+]/sgl⁰⁸³¹⁰

(Haerry et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

A W^GMR.PU background generates a severe ventral-to-dorsal misrouting phenotype in dsh⁴ mutant clone retina.

(Sato et al., 2006)

The dsh⁴ phenotype is dominantly enhanced by wg^en11, sgl^SG9 and sgl⁰⁸³¹⁰; dsh⁴/Y flies that are also heterozygous for wg^en11, sgl^SG9 or sgl⁰⁸³¹⁰ show defects in adult structures in 62%, 19% and 41% of cases respectively.

(Haerry et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lefevre.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

dsh⁴

dsh^VA135

(Song and Xie, 2003)

dsh^VA153

(Sato et al., 2006, Cohen et al., 2002, Nakagoshi et al., 2002, Fujise et al., 2001, Sato and Saigo, 2000, Cavodeassi et al., 1999, Heslip et al., 1997, Lecuit and Cohen, 1997, Neumann and Cohen, 1997, Royet and Finkelstein, 1997, Klingensmith et al., 1994, Theisen et al., 1994, Perrimon and Mahowald, 1987)

dsh^va153

(Tomoyasu et al., 2000, Haerry et al., 1997)

l(1)VA153

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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