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General Information
Symbol
Dmel\esc2
Species
D. melanogaster
Name
FlyBase ID
FBal0003822
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

10bp of wild type sequence are replaced by 12bp in esc2.

Inserted_sequence:

AGATGACGCCAC

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

10bp of wild type sequence are replaced by 12bp, causing 22 C-terminal amino acids to be replaced by a different stretch of 48 amino acids. Amino acid replacement: Q184K. Mutation is a natural polymorphism.

10bp deletion replaced by a 12bp insertion of unknown origin beginning after the first base of the V404 codon within WD5. Insertion results in a frame shift deleting the C terminal 22 residues and replacing it with 44 novel residues.

Carried on aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

All denticle belts of embryos derived from a esc2/esc5 stock are rectangular in shape, indicating a transformation to the eighth abdominal segment. Midgut constrictions are partial or not formed in these embryos.

Embryos derived from esc2/Df(2L)esc10 parents die at the end of embryogenesis with all segments transformed towards the eighth abdominal segment.

Heterozygotes with Df(2L)esc10 are embryonic lethal due to transformation of most body segments into ectopic eighth abdominal segments. Complete lethality is seen if a 2 hour heat shock of eschs.PS is administered after 4 hours of embryogenesis. Rescue to first instar larval viability is seen if heat shock is induced prior to 4 hours of embryogenesis. Only 10% of rescued larvae reach adulthood, of these adults half are normal and half exhibit segmental transformations.

Homozygous embryos are viable and show no cuticular defects.

After 9 hours of embryogenesis abd-A is expressed throughout the A-P axis including the head and ectopic Abd-B expression is uniform, consequently all the segments to be transformed into the normal eighth adult abdominal segment. esc+/esc- embryos from esc10/esc2 females show complete or partial transformation of the segments to the eighth. A gradient of abd-A expression is seen: strong in the thorax and abdomen but weak in the head.

Lack midgut constrictions.

Less than 10% of esc2/Dp(2;2)GYL animals (having two copies of paternally derived zygotic esc function, but no maternal esc function) survive past the third larval instar stage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

esc2 has leg phenotype, enhanceable by Mmus\EedF6.αTub84B

esc2 has leg phenotype, enhanceable by Mmus\Eedhs.PW

Suppressed by
Statement
Reference

esc2 has phenotype, suppressible by Pcoe\escesc.UTR

Suppressor of
Statement
Reference

esc2 is a suppressor of eye phenotype of Scer\GAL4lz-gal4, sensUAS.cNa

esc2/esc[+] is a suppressor | partially of adult abdominal segment 4 | ectopic phenotype of E(z)Trm

esc2/esc[+] is a suppressor | partially of adult abdominal segment 5 phenotype of E(z)Trm

esc2/esc[+] is a suppressor | partially of adult mesothoracic segment | ectopic phenotype of E(z)Trm

esc2/esc[+] is a suppressor | partially of adult metathoracic segment phenotype of E(z)Trm

esc2/esc[+] is a suppressor | partially of adult prothoracic segment phenotype of E(z)Trm

Additional Comments
Genetic Interactions
Statement
Reference

The disorganised eye phenotype caused by expression of sensScer\UAS.cNa under the control of Scer\GAL4lz-gal4 is suppressed by esc2.

Xenogenetic Interactions
Statement
Reference

The addition of Mmus\eedF6.αTub84B or Mmus\eedhs.PW to esc6/esc2 males causes an enhancement of the leg transformation phenotype seen in esc6/esc2 alone. The maternal effect embryonic lethality and transformation phenotypes seen embryos laid by esc6/esc2 mothers is not rescued by the addition of Mmus\eedF6.αTub84B or Mmus\eedhs.PW. In these embryos, all three thoracic and the first seven abdominal segments are transformed to the eight abdominal segment identity. In addition, the head fails to undergo involution and two additional segments, maxillary segment and cephalic segment can be seen.

Approximately two-thirds of embryos derived from esc2/Df(2L)esc10 parents that are injected with Pcoe\escesc.UTR RNA show some phenotypic rescue, and one-third show normal or nearly normal development.

Complementation and Rescue Data
Partially rescued by

esc2/Df(2L)esc10 is partially rescued by escVE212AA

esc2/Df(2L)esc10 is partially rescued by esccNa

esc2/Df(2L)esc10 is partially rescued by escRDE216AAA

esc2/Df(2L)esc10 is partially rescued by escGG210AA

esc2/Df(2L)esc10 is partially rescued by escRD282AA

esc2/Df(2L)esc10 is partially rescued by eschs.PS

Comments

The segment transformation phenotype is at least partially rescued by injection of esccNa RNA; the majority of injected embryos form head skeletal structures and differentiated thoracic and abdominal denticle bands, and sometimes the larvae survive to adulthood. Embryos derived from esc2/Df(2L)esc10 parents are not rescued by injection of mutant escΔGH, escΔWD7 or escRDE216AAA:DFST278AFAA RNA. Some rescue of embryos derived from esc2/Df(2L)esc10 parents is seen if they are injected with escGG210AA, escVE212AA, escRDE216AAA, escDFST278AFAA or escRD282AA RNA.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Struhl.

Arose on: In(2LR)O.

Comments
Comments

The expression of Antp, Scr and Ubx protein in esc- mutants in imaginal discs and the central nervous system has been studied.

Ubx, Scr and Antp, protein expression has been studied in esc2/esc5 embryos lacking both maternal and zygotic esc function, and in esc2/Dp(2;2)GYL embryos lacking maternal but having two copies of paternally derived zygotic esc function.

Embryos initially have normal abd-A expression pattern.

Expression of en in the embryo not derepressed, as it is for other Pc group genes, but all stripes resemble A8.

Does not suppress the nos mutant phenotype.

Carried on a CyO chromosome.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (38)