FlyBase Allele Report: Dmel\esc[2]

General Information

Symbol

Dmel\esc²

Species

D. melanogaster

Name

FlyBase ID

FBal0003822

Feature type

allele

Associated gene

Dmel\esc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Gindhart and Kaufman, 1995, Cheng et al., 1994, McKeon and Brock, 1991, Struhl and Akam, 1985)

Mutagen

spontaneous

(Sathe and Harte, 1995)

Progenitor genotype

Cytology

Description

10bp of wild type sequence are replaced by 12bp, causing 22 C-terminal amino acids to be replaced by a different stretch of 48 amino acids. Amino acid replacement: Q184K. Mutation is a natural polymorphism.

(Gutjahr et al., 1995)

10bp deletion replaced by a 12bp insertion of unknown origin beginning after the first base of the V404 codon within WD5. Insertion results in a frame shift deleting the C terminal 22 residues and replacing it with 44 novel residues.

(Sathe and Harte, 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:11,827,518..11,827,527 [-]

Comment:

10bp of wild type sequence are replaced by 12bp in esc².

Inserted_sequence:

AGATGACGCCAC

(Gutjahr et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates leukemia

modeled by abd-A^UAS.Tag:HA

(Ponrathnam et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment 1 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 2 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 3 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 4 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 5 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 6 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 7 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 8 | ectopic

(Simon et al., 1992)

abdominal segment 8 | ectopic (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 9 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 10 (with Df(2L)esc10)

(Ng et al., 1997)

abdominal segment 11 (with Df(2L)esc10)

(Ng et al., 1997)

embryonic abdominal segment 8 | ectopic | maternal effect (with esc⁵)

(Choi et al., 2000)

embryonic midgut constriction | maternal effect (with esc⁵)

(Choi et al., 2000)

mesothoracic segment (with Df(2L)esc10)

(Ng et al., 1997)

metathoracic segment (with Df(2L)esc10)

(Ng et al., 1997)

prothoracic segment (with Df(2L)esc10)

(Ng et al., 1997)

segment

(Simon et al., 1992)

ventral denticle belt | maternal effect (with esc⁵)

(Choi et al., 2000)

Detailed Description

Statement

Reference

All denticle belts of embryos derived from a esc²/esc⁵ stock are rectangular in shape, indicating a transformation to the eighth abdominal segment. Midgut constrictions are partial or not formed in these embryos.

(Choi et al., 2000)

Embryos derived from esc²/Df(2L)esc10 parents die at the end of embryogenesis with all segments transformed towards the eighth abdominal segment.

(Ng et al., 1997)

Heterozygotes with Df(2L)esc10 are embryonic lethal due to transformation of most body segments into ectopic eighth abdominal segments. Complete lethality is seen if a 2 hour heat shock of esc^hs.PS is administered after 4 hours of embryogenesis. Rescue to first instar larval viability is seen if heat shock is induced prior to 4 hours of embryogenesis. Only 10% of rescued larvae reach adulthood, of these adults half are normal and half exhibit segmental transformations.

(Simon et al., 1995)

Homozygous embryos are viable and show no cuticular defects.

(Soto et al., 1995)

After 9 hours of embryogenesis abd-A is expressed throughout the A-P axis including the head and ectopic Abd-B expression is uniform, consequently all the segments to be transformed into the normal eighth adult abdominal segment. esc⁺/esc^- embryos from esc¹⁰/esc² females show complete or partial transformation of the segments to the eighth. A gradient of abd-A expression is seen: strong in the thorax and abdomen but weak in the head.

(Simon et al., 1992)

Lack midgut constrictions.

(McKeon and Brock, 1991)

Less than 10% of esc²/Dp(2;2)GYL animals (having two copies of paternally derived zygotic esc function, but no maternal esc function) survive past the third larval instar stage.

(Glicksman and Brower, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

esc² is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

esc² has leg phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic thoracic segment phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic maxillary segment | ectopic phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic procephalic segment | ectopic phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has leg phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic thoracic segment phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 1 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 2 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 3 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 4 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 5 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 6 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 1 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 7 phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 8 | ectopic phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic maxillary segment | ectopic phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic procephalic segment | ectopic phenotype, enhanceable by Mmus\Eed^hs.PW

(Wang et al., 2000)

esc² has embryonic abdominal segment 2 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 3 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 4 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 5 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 6 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 7 phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc² has embryonic abdominal segment 8 | ectopic phenotype, enhanceable by Mmus\Eed^F6.αTub84B

(Wang et al., 2000)

esc²/esc⁵ has sex comb | ectopic phenotype, enhanceable by E(z)^+t8.8

(Campbell et al., 1995)

Suppressor of

Statement

Reference

esc² is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

esc²/esc[+] is a suppressor of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

esc²/esc[+] is a suppressor of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

esc²/esc[+] is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

esc²/esc[+] is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

esc²/esc[+] is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The disorganised eye phenotype caused by expression of sens^Scer\UAS.cNa under the control of Scer\GAL4^lz-gal4 is suppressed by esc².

(Anderson et al., 2011)

Xenogenetic Interactions

Statement

Reference

The addition of Mmus\eed^F6.αTub84B or Mmus\eed^hs.PW to esc⁶/esc² males causes an enhancement of the leg transformation phenotype seen in esc⁶/esc² alone. The maternal effect embryonic lethality and transformation phenotypes seen embryos laid by esc⁶/esc² mothers is not rescued by the addition of Mmus\eed^F6.αTub84B or Mmus\eed^hs.PW. In these embryos, all three thoracic and the first seven abdominal segments are transformed to the eight abdominal segment identity. In addition, the head fails to undergo involution and two additional segments, maxillary segment and cephalic segment can be seen.

(Wang et al., 2000)

Approximately two-thirds of embryos derived from esc²/Df(2L)esc10 parents that are injected with Pcoe\esc^esc.UTR RNA show some phenotypic rescue, and one-third show normal or nearly normal development.

(Ng et al., 1997)

Complementation and Rescue Data

Rescued by

esc¹⁰/esc² is rescued by esc^{lexA.αTub84B}

(Poux et al., 2001)

esc¹⁰/esc² is rescued by esc^lexA.hs

(Poux et al., 2001)

Partially rescued by

esc²/Df(2L)esc10 is partially rescued by esc^hs.PS

(Simon et al., 1995)

Not rescued by

esc²/Df(2L)esc10 is not rescued by esc^ΔGH

(Ng et al., 1997)

Comments

The segment transformation phenotype is at least partially rescued by injection of esc^cNa RNA; the majority of injected embryos form head skeletal structures and differentiated thoracic and abdominal denticle bands, and sometimes the larvae survive to adulthood. Embryos derived from esc²/Df(2L)esc10 parents are not rescued by injection of mutant esc^ΔGH, esc^ΔWD7 or esc^{RDE216AAA:DFST278AFAA} RNA. Some rescue of embryos derived from esc²/Df(2L)esc10 parents is seen if they are injected with esc^GG210AA, esc^VE212AA, esc^RDE216AAA, esc^DFST278AFAA or esc^RD282AA RNA.

(Ng et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Struhl.

Arose on: In(2LR)O.

Comments

The expression of Antp, Scr and Ubx protein in esc^- mutants in imaginal discs and the central nervous system has been studied.

(Glicksman and Brower, 1988)

Ubx, Scr and Antp, protein expression has been studied in esc²/esc⁵ embryos lacking both maternal and zygotic esc function, and in esc²/Dp(2;2)GYL embryos lacking maternal but having two copies of paternally derived zygotic esc function.

(Glicksman and Brower, 1990)

Embryos initially have normal abd-A expression pattern.

(Simon et al., 1992)

Expression of en in the embryo not derepressed, as it is for other Pc group genes, but all stripes resemble A8.

(Moazed and O'Farrell, 1992)

Does not suppress the nos mutant phenotype.

(Pelegri and Lehmann, 1994)

Carried on a CyO chromosome.

(Gutjahr et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

esc⁰

(Bonnet et al., 2026)

esc²

(Bonnet et al., 2026, Ponrathnam et al., 2021, Finogenova et al., 2020, Bengani et al., 2013, Vasanthi et al., 2013, Anderson et al., 2011, Chopra et al., 2011, Sultana et al., 2011, Ketel et al., 2005, Fitzgerald and Bender, 2001, Choi et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (42)

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