10bp of wild type sequence are replaced by 12bp, causing 22 C-terminal amino acids to be replaced by a different stretch of 48 amino acids. Amino acid replacement: Q184K. Mutation is a natural polymorphism.
10bp deletion replaced by a 12bp insertion of unknown origin beginning after the first base of the V404 codon within WD5. Insertion results in a frame shift deleting the C terminal 22 residues and replacing it with 44 novel residues.
The addition of Mmus\eedF6.αTub84B or Mmus\eedhs.PW to esc6/esc2 males causes an enhancement of the leg transformation phenotype seen in esc6/esc2 alone. The maternal effect embryonic lethality and transformation phenotypes seen embryos laid by esc6/esc2 mothers is not rescued by the addition of Mmus\eedF6.αTub84B or Mmus\eedhs.PW. In these embryos, all three thoracic and the first seven abdominal segments are transformed to the eight abdominal segment identity. In addition, the head fails to undergo involution and two additional segments, maxillary segment and cephalic segment can be seen.
The segment transformation phenotype is at least partially rescued by injection of esccNa RNA; the majority of injected embryos form head skeletal structures and differentiated thoracic and abdominal denticle bands, and sometimes the larvae survive to adulthood. Embryos derived from esc2/Df(2L)esc10 parents are not rescued by injection of mutant escΔGH, escΔWD7 or escRDE216AAA:DFST278AFAA RNA. Some rescue of embryos derived from esc2/Df(2L)esc10 parents is seen if they are injected with escGG210AA, escVE212AA, escRDE216AAA, escDFST278AFAA or escRD282AA RNA.