FlyBase Allele Report: Dmel\CrebB[S162]

General Information

Symbol

Dmel\CrebB^S162

Species

D. melanogaster

Name

FlyBase ID

FBal0006239

Feature type

allele

Associated gene

Dmel\CrebB

Associated Insertion(s)

Carried in Construct

Also Known As

S162, dCreb2^S162, CrebB-17A^S162

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Eberl et al., 1992)

Progenitor genotype

Cytology

Description

The S162 mutation is located late in the CrebB open reading frame, truncating about 60 amino acids from any protein isoform that contains the C-terminal bZIP domain.

(Fropf et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q48.ex1p.UAS

(Iijima-Ando et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult

(Belvin et al., 1999)

NMJ bouton | third instar larval stage

(Chen and Ganetzky, 2012)

Detailed Description

Statement

Reference

There is a significant decrease in synaptic bouton numbers at larval neuromuscular junctions (NMJs), compared with wild-type, in CrebB-17A^S162 mutants.

Heterozygous CrebB-17A^S162 larvae have normal NMJs.

(Chen and Ganetzky, 2012)

Homozygous CrebB-17A^S162 mutants are lethal.

A single copy of CrebB-17A^S162 does not cause any lethality.

(Iijima-Ando et al., 2005)

Mutant flies show a significant increase in the number of rest hours per 24 hours compared to controls.

Mutant flies show significantly increased and prolonged rest rebound after rest deprivation compared to controls.

(Hendricks et al., 2001)

CrebB-17A^S162, though lethal, is not completely penetrant, less than 0.5% of flies survive to adulthood. These escapers are about three quarters the size of wild type flies, but otherwise normal in appearance. Of 34 mutant flies tested for circadian locomotor activity, 13 (38%) were arrhythmic and the rest showed a short period averaging 22.8 hours.

(Belvin et al., 1999)

Germline clones demonstrate no maternal effect. Zygotic lethality acts in the third larval instar stage.

(Eberl et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CrebB^S162/CrebB-17A[+] is an enhancer of partially lethal - majority die phenotype of Hsap\HTT^Q48.ex1p.UAS, Scer\GAL4^elav-C155

(Iijima-Ando et al., 2005)

Other

Statement

Reference

CrebB^S162, Df(1)Exel9051/+ has abnormal neuroanatomy | third instar larval stage phenotype

(Chen and Ganetzky, 2012)

CrebB^S162/CrebB[+], Hsap\HTT^Q22.ex1p.UAS, Scer\GAL4^elav-C155 has viable phenotype

(Iijima-Ando et al., 2005)

CrebB^S162, Hsap\HTT^Q48.ex1p.UAS, Scer\GAL4^elav-C155 has lethal phenotype

(Iijima-Ando et al., 2005)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

CrebB^S162, Scer\GAL4^30A is a non-enhancer of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressor of

Statement

Reference

CrebB^S162 is a suppressor of eye phenotype of Crtc^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2008)

NOT Suppressor of

Statement

Reference

CrebB^S162, Scer\GAL4^30A is a non-suppressor of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Other

Statement

Reference

CrebB^S162, Df(1)Exel9051/+ has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chen and Ganetzky, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for both CrebB-17A^S162 and Df(1)Exel9051 results in a significant reduction in neuromuscular junction (NMJ) growth.

(Chen and Ganetzky, 2012)

A CrebB-17A^S162 background suppresses the rough eye phenotype found in TORC^Scer\UAS.cWa-expressing (under the control of Scer\GAL4^GMR.PF) flies.

(Wang et al., 2008)

Xenogenetic Interactions

Statement

Reference

The survival rate of flies expressing Hsap\HD^{Q48.ex1p.Scer\UAS} in the nervous system (under the regulation of Scer\GAL4^elav-C155) is sensitive to the gene dosage of CrebB-17A^S162 flies. A CrebB-17A^S162 heterozygous background more than doubles the lethality of Hsap\HD^{Q48.ex1p.Scer\UAS} flies compared to flies expressing Hsap\HD^{Q48.ex1p.Scer\UAS} alone (under the control of Scer\GAL4^elav-C155).

Addition of a heterozygous CrebB-17A^S162 background to CrebB-17A^+tIa Hsap\HD^{Q48.ex1p.Scer\UAS} (Scer\GAL4^elav-C155) flies reduces the survival rate slightly, although lethality is not as high as in Hsap\HD^{Q48.ex1p.Scer\UAS} (Scer\GAL4^elav-C155) flies.

The survival rate of flies expressing Hsap\HD^{Q22.ex1p.Scer\UAS} in the nervous system (under the regulation of Scer\GAL4^elav-C155) is not sensitive to the gene dosage of CrebB-17A^S162 flies.

Homozygous CrebB-17A^S162 flies expressing Hsap\HD^{Q48.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155 are lethal. Addition of the CrebB-17A^+t.STOP transgene to homozygous CrebB-17A^S162 flies expressing Hsap\HD^{Q48.ex1p.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in an approximate 1% survival rate.

(Iijima-Ando et al., 2005)

Complementation and Rescue Data

Rescued by

CrebB^S162 is rescued by CrebB^+tIa

(Iijima-Ando et al., 2005)

CrebB^S162 is rescued by CrebB^d.hs

(Belvin et al., 1999)

Not rescued by

CrebB^S162 is not rescued by CrebB^+t.STOP

(Iijima-Ando et al., 2005)

Comments

Lethality can be almost fully rescued by an induced transgene carrying CrebB-17A, partial rescue is achieved with the non-induced transgene. Interpretation of rescue experiments is complicated as background genetic variability also gives partial rescue.

(Yin, 1997)

Images (0)

Mutant

Wild-type

Stocks (2)

4720

107845

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CREB^S162

(Iijima-Ando et al., 2009)

CrebB-17A^S162

(Zhang et al., 2013, Kang et al., 2011, Popodi et al., 2010-)

CrebB^S162

(Kuntz et al., 2017, Graves et al., 2015, Fox et al., 2010, Wang et al., 2008)

DCREB^S162

(Stanewsky, 2003)

S162

(Fropf et al., 2013, Iijima-Ando et al., 2005, Hendricks et al., 2001, Belvin et al., 1999, Eberl et al., 1992)

creb B-17A^S162

(Kiger et al., 2001)

dCREB^S162

(Iijima-Ando et al., 2009)

dCreb2^S162

(Tubon et al., 2013, Chen and Ganetzky, 2012)

l(1)17Af¹

l(1)S162

s162

(Hong et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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