FlyBase Allele Report: Dmel\lt[11]

General Information

Symbol

Dmel\lt¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0011687

Feature type

allele

Associated gene

Dmel\lt

Associated Insertion(s)

Carried in Construct

Also Known As

lt^40-12, EMS40-12

Key Links

Allele class

hypomorphic allele - genetic evidence

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wakimoto, 2008.11.19)

hypomorphic allele - genetic evidence

(Wakimoto and Hearn, 1990)

Mutagen

ethyl methanesulfonate

(Wakimoto and Hearn, 1990, Hilliker, 1976)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color | recessive

(Clegg et al., 1998, Warner et al., 1998)

abnormal size | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

lethal

(Devlin et al., 1990)

lethal | dominant

(Wakimoto and Hearn, 1990)

Phenotype Manifest In

autolysosome | wandering third instar larval stage | nutrition conditional (with Df(2L)lt45)

(Takáts et al., 2014)

autophagosome | increased number | wandering third instar larval stage | nutrition conditional (with Df(2L)lt45)

(Takáts et al., 2014)

embryonic/larval fat body | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

embryonic/larval fat body | wandering third instar larval stage | nutrition conditional (with Df(2L)lt45)

(Takáts et al., 2014)

embryonic/larval garland cell | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

embryonic/larval salivary gland | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

larval brain | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

late endosome | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

lysosome | third instar larval stage (with Df(2L)lt45)

(Lund et al., 2018)

pigment cell

(Clegg et al., 1998, Warner et al., 1998)

Detailed Description

Statement

Reference

lt¹¹/Df(2L)lt45 mutant larvae exhibit trafficking defects in the brain, fat body, salivary glands and garland cells. In the brain, the lysosomal marker Lamp mislocalizes to cytoplasmic granules. In the salivary gland, the lysosomal marker Lamp mislocalizes to vesicles instead of showing the normal localization to the Lysotracker-positive tubulo-vesicular lysosomal network, and the lysosomal network seems fragmented. In the fat body, the lysosomal marker Lamp shows some mislocalization to vesicles instead of showing the normal localization to large, Lysotracker-positive autolysosomes, which are much smaller than in controls. In the Garland cells there are enlarged vacuoles and postendocytic trafficking defects (e.g. endocytozed proteins fail to be efficiently incorporated into Lysotracker-positive autolysosomes), as compared to controls.

(Lund et al., 2018)

Autolysosome formation is inhibited in the fat body cells of starved lt¹¹/Df(2L)lt45 mutant wandering third instar larvae. Large number of autophagosomes are observed. Numerous dense organelles are seen, which are usually round and smaller than the autolysosomes observed in controls. A subset of these vesicles contain recognisable remnants of cytoplasmic material, such as mitochondrion.

(Takáts et al., 2014)

lt^hdS1A/lt¹¹ flies have 60% wild-type viability.

(Devlin et al., 1990)

Reduced eye and Malpighian tubule cell pigmentation, variable crumpled wings and missing bristles. Moderate position effect variegation is seen with lt rearrangements.

(Wakimoto and Hearn, 1990)

Hemizygotes die at late pupal stages.

(Hilliker, 1976)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208¹/Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208¹/Su(var)2-1^v9

(Clegg et al., 1998)

lt¹¹ has abnormal eye color | recessive phenotype, enhanceable by Su(var)205¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹¹ has abnormal eye color | recessive phenotype, enhanceable by Su(var)208¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)208¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹¹ has abnormal eye color | recessive phenotype, enhanceable by Su(var)208¹/Su(var)205¹

(Clegg et al., 1998)

lt¹¹ has abnormal eye color | recessive phenotype, enhanceable by Su(var)208¹/Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹¹ has abnormal eye color | recessive phenotype, enhanceable by Su(var)208¹/Su(var)2-1^v9

(Clegg et al., 1998)

lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)208¹/Su(var)2-1^v9

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)2-1^v9

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)205[+]/E(Pc)[+]/Su(var)205¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)208[+]/Su(var)208¹/E(Pc)[+]/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹/lt¹¹ has abnormal eye color phenotype, enhanceable by Su(var)208[+]/Su(var)208¹/Su(var)205[+]/Su(var)205¹

(Clegg et al., 1998)

Other

Statement

Reference

E(Pc)^unspecified, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1⁰¹, lt¹¹ has viable phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1⁰¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

Su(var)2-1^v9, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

Dp(2;Y)G, Su(var)2-1^v9, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

E(lt^v)335³³⁵, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

Dp(2;Y)G, E(Pc)^unspecified, Su(var)208¹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

Su(var)2-1^v9/Su(var)2-1⁰¹, lt¹¹ has partially lethal | recessive | male phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)319³¹⁹, lt¹¹ has viable phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1^v9, lt¹¹ has viable phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1^v9, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)208¹, lt¹¹ has partially lethal phenotype

(Clegg et al., 1998)

E(lt^v)335³³⁵, Su(var)208¹, lt¹¹ has partially lethal phenotype

(Clegg et al., 1998)

E(lt^v)335³³⁵, Su(var)2-1^v9, lt¹¹ has partially lethal phenotype

(Clegg et al., 1998)

Su(var)2-1^v9, Su(var)205¹, lt¹/lt¹¹ has partially lethal phenotype

(Clegg et al., 1998)

Su(var)205¹, Su(var)208¹, lt¹/lt¹¹ has partially lethal phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)319³¹⁹, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)335³³⁵, lt¹¹ has viable phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)335³³⁵, lt¹¹ has abnormal eye color phenotype

(Clegg et al., 1998)

car¹, lt¹/lt¹¹ has lethal | recessive phenotype

(Warner et al., 1998)

dor¹, lt¹/lt¹¹ has lethal | recessive phenotype

(Warner et al., 1998)

g^53d, lt¹/lt¹¹ has abnormal eye color | recessive phenotype

(Warner et al., 1998)

g^53d, lt¹¹ has abnormal eye color | recessive phenotype

(Warner et al., 1998)

car¹, lt¹/lt¹¹ has lethal phenotype

(Warner et al., 1998)

dor¹, lt¹/lt¹¹ has lethal phenotype

(Warner et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208¹/Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)2-1[+]/Su(var)208[+]/Su(var)208¹/Su(var)2-1^v9

(Clegg et al., 1998)

lt¹¹ has pigment cell phenotype, enhanceable by Su(var)205¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)2-1^v9

(Clegg et al., 1998)

lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208¹/Su(var)205¹

(Clegg et al., 1998)

lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208¹/Su(var)2-1⁰¹

(Clegg et al., 1998)

lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208¹/Su(var)2-1^v9

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)205[+]/E(Pc)[+]/Su(var)205¹/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208[+]/Su(var)208¹/E(Pc)[+]/E(Pc)^unspecified

(Clegg et al., 1998)

lt¹/lt¹¹ has pigment cell phenotype, enhanceable by Su(var)208[+]/Su(var)208¹/Su(var)205[+]/Su(var)205¹

(Clegg et al., 1998)

Other

Statement

Reference

E(Pc)^unspecified, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1⁰¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

Su(var)2-1^v9, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

Dp(2;Y)G, Su(var)2-1^v9, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

Dp(2;Y)G, E(Pc)^unspecified, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(lt^v)335³³⁵, Su(var)208¹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(lt^v)319³¹⁹, Su(var)2-1^v9, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)319³¹⁹, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

E(Pc)^unspecified, E(lt^v)335³³⁵, lt¹¹ has pigment cell phenotype

(Clegg et al., 1998)

g^53d, lt¹/lt¹¹ has pigment cell phenotype

(Warner et al., 1998)

g^53d, lt¹¹ has pigment cell phenotype

(Warner et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Eye pigmentation in lt¹¹/lt¹ flies is reduced if the flies are also carrying E(Pc)^unspecified/Su(var)205¹, E(Pc)^unspecified/Su(var)208^unspecified, Su(var)208^unspecified/Su(var)205¹, Su(var)208^unspecified/Su(var)2-1¹, Su(var)2-1^v9/Su(var)2-1¹ or Su(var)2-1^v9/Su(var)208^unspecified. Eye pigmentation in lt¹¹/+ flies that are also carrying E(Pc)^unspecified/Su(var)208^unspecified or Su(var)2-1^v9/Su(var)208^unspecified is reduced. Pigmentation is restored to wild-type levels in these flies by Dp(2;Y)G. XY lt¹¹/lt¹; Su(var)2-1^v9/Su(var)2-1¹ males have reduced viability, which is restored in XO males. lt¹¹/lt¹; Su(var)2-1^v9/Su(var)205¹ flies have reduced viability. Viability is partially restored in XO males. lt¹¹/lt¹; Su(var)208^unspecified/Su(var)205¹ flies have reduced viability. Viability is restored in XO males. Eye pigmentation is reduced and viability is essentially wild-type in lt¹¹/+ flies that are also carrying E(Pc)^unspecified and E(lt^v)319³¹⁹, E(Pc)^unspecified and E(lt^v)335³³⁵ or Su(var)2-1^v9 and E(lt^v)319³¹⁹. Eye pigmentation and viability is reduced in lt¹¹/+ flies that are also carrying Su(var)208^unspecified and E(lt^v)319³¹⁹ or Su(var)208^unspecified and E(lt^v)335³³⁵. Eye pigmentation is nearly wild-type and viability is reduced in lt¹¹/+ flies that are also carrying Su(var)2-1^v9 and E(lt^v)335³³⁵.

(Clegg et al., 1998)

Eye colour: lt¹/lt¹¹ g^53d double mutants show a reduction in red eye pigment compared to either lt¹/lt¹¹ or g^53d single mutants.

(Warner et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2L)C'/lt¹¹ is rescued by lt^{+tCH321-64G01}

(Venken et al., 2009)

Not rescued by

Df(2L)C'/lt¹¹ is not rescued by lt^tCH321-16H04

(Venken et al., 2009)

Df(2L)C'/lt¹¹ is not rescued by lt^tCH321-05E14

(Venken et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26262

lt¹¹/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Notes on Origin

Discoverer

Hilliker.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

40-12

(Howe et al., 1995)

EMS40-12

(Devlin et al., 1990, Hilliker, 1976)

l(2)40Fb^40-12

(Wakimoto and Hearn, 1990)

l(2)EMS40-12

lt¹¹

(Lund et al., 2021, Lund et al., 2018, Takáts et al., 2015, Venken et al., 2009, Wakimoto, 2008.11.19)

lt^40-12

(Clegg et al., 1998, Warner et al., 1998)

lt[11]

(Takáts et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections