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General Information
Symbol
Dmel\mle1
Species
D. melanogaster
Name
FlyBase ID
FBal0012332
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
mle
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

A single G nucleotide is deleted at position 329 of mle cDNA GB:M74121. This leads to a frameshift, with the first stop codon at aa 125.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A single G nucleotide is deleted at position 329 of mle cDNA GB:M74121. This leads to a frameshift, with the first stop codon at amino acid residue 125.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mle1 mutants display strong X chromosome condensation.

Male lethal at the late third instar to early pupal stage. Homozygous female larvae are viable.

mle1/mle6 males are temperature sensitive. At 18oC no significant lethality or sterility is observed. At 25oC males are viable but sterile, sperm are fully formed but non-motile. At 29oC no males reach adulthood, they die during late third instar and pupal stages. Homozygous males shows morphological signs that spermatogenesis has advanced to post-meiotic stages.

mleGET allows male to survive to mid to late larval stages, the same as males not carrying mleGET. A few male that do survive to adulthood are sterile and hold out their wings and have disorganised abdominal bristles.

Homozygous males show abnormal dosage compensation as determined by tritiated uridine labeling of polytene chromosomes.

male-lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

msl-11, msl-1[+], msl-2[+], msl-21, mle1, mle[+] is an enhancer of lethal | male phenotype of Trl62/Trl13C

NOT Enhancer of
Statement
Reference

mle1/mle[+] is a non-enhancer of lethal | male phenotype of Trl2.3/Trl13C

mle1/mle[+] is a non-enhancer of partially lethal | female phenotype of Trl2.3/Trl13C

msl-11, msl-1[+], msl-2[+], msl-21, mle1, mle[+] is a non-enhancer of partially lethal | female phenotype of Trl62/Trl13C

Suppressor of
Statement
Reference

mle1/mle[+] is a suppressor | partially of lethal | male phenotype of ocmL1658N/ocmG1646E

NOT Suppressor of
Statement
Reference

msl-11, msl-1[+], msl-2[+], mle1, msl-21, mle[+] is a non-suppressor of partially lethal | female phenotype of Trl2.3/Trl13C

mle1/mle[+] is a non-suppressor of partially lethal | female phenotype of Trl62/Trl13C

Other
Phenotype Manifest In
Suppressed by
Suppressor of
Statement
Reference

mle1 is a suppressor of X chromosome | male phenotype of Iswi2

mle1 is a suppressor of ovary phenotype of ovoD1rv22

mle14/mle1 is a suppressor of ovary phenotype of ovoD1rv22

mle1 is a suppressor of ovary phenotype of ovoD1rv23

mle14/mle1 is a suppressor of ovary phenotype of ovoD1rv23

mle5/mle1 is a suppressor of ovary phenotype of ovoD1rv23

Other
Additional Comments
Genetic Interactions
Statement
Reference

The viability of SxlfP7B0/+ females is not affected if they also carry one maternally-derived copy of mle1.

One maternally-derived copy of mle1 significantly reduces the viability of scsisB-1 sisA1/+ females so that they show almost complete lethality. One paternally-derived copy of mle1 significantly reduces the viability of scsisB-1 sisA1/+ females, although this zygotic effect is generally weaker than the maternal one.

The presence of Su(var)3-7FL.hs.T:Ivir\HA1 suppresses the mle1 phenotype resulting in almost normal male X chromosome morphology.

Homozygosity for mle1 suppresses the bloated X chromosome phenotype seen in polytene chromosome squashes from male homozygous Su(var)3-714 third instar larvae.

mle1 completely suppresses the morphological defects of the X chromosome that are seen in salivary gland polytene chromosome preparations from Iswi2 males.

Ovaries of homozygous ovoD1rv23 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle1/mle5 or mle1/mle14. Ovaries of ovoD1rv23/ovoD1rv22 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle1/mle1. Ovaries of homozygous ovoD1rv22 females contain germline chambers in less than 20% of cases. This phenotype is partially suppressed if the females are also carrying mle1/mle1 or mle1/mle14. ovoD1rv22/ovoD1rv22; mle1/mle1 ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by SxlM1; ovaries more often contain germ cells of female morphology rather than male morphology. ovoD1rv23/ovoD1rv23; mle1/mle5 ovaries most often contain germ cells of male morphology rather than female morphology. This phenotype is partially suppressed by SxlM1; ovaries more often contain germ cells of female morphology rather than male morphology.

The survival of 2X ovoD1rv23/ovoD1rv22 germline cells is enhanced in an mle1/mle5 background. Surviving cells usually form small ovarian tumours.

Some females homozygous for mle1 and heterozygous for Sxlf1 have sex combs on the basitarsi of their forelegs. This phenotype occurs with a higher frequency in females with homozygous mle1 mothers.

Sxlf1/+ chromosomal females also homozygous or heterozygous mle1 show variable transformation of sexually dimorphic structures to male characteristics. The frequency of transformation is not high, is higher in females homozygous (rather than heterozygous) for mle1, and expression is incomplete. The frequency of transformation is higher in female flies with mothers homozygous mle1. Sex combs are only seen in Sxlf1/+ females homozygous for mle1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

mle1 is partially rescued by mleΔgly

mle1 is partially rescued by mleΔgly

Comments

The lethality of mle1 homozygous males is rescued by mleΔgly, but the rescued males are sterile.

mle+t10.2 fails to rescue the lethality of mle1. The mutant die during late larval stages.

mle+t10.2 rescues the lethality of mle1.

Mutant phenotype can be rescued by P element mediated transformation of a wild type mle gene copy.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Oshima, 1971.

Isolated from: Kofu, Japan population, 1971. Separable from: mfs(2)3501. Recovered as the "350" second chromosome which carries both mfs(2)3501 and mle1.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (39)