FlyBase Allele Report: Dmel\aub[sting-1]

General Information

Symbol

Dmel\aub^sting-1

Species

D. melanogaster

Name

FlyBase ID

FBal0012506

Feature type

allele

Associated gene

Dmel\aub

Associated Insertion(s)

P{lacW}aub^sting-1

Carried in Construct

Also Known As

aub^sting

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

neomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Bozzetti et al., 2015)

neomorphic allele - molecular evidence

(Bozzetti et al., 2015)

Mutagen

P-element activity

(Schmidt et al., 1999, Schmidt et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lacW}aub^sting-1

Cytology

Description

aub^sting-1 acts as a loss of function allele (reduced expression) in the germline and as a gain of function allele (due to ectopic expression) in the somatic hub cells.

(Bozzetti et al., 2015)

Insertion of a P{lacW} element 58bp upstream of the transcriptional start site.

(Schmidt et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:11,001,423..11,001,423

(Sahin et al., 2016, Aravin et al., 2004, Tritto et al., 2003, Schmidt et al., 1999, Schmidt et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | recessive | third instar larval stage

(Bozzetti et al., 2015)

abnormal size | recessive | third instar larval stage

(Bozzetti et al., 2015)

male sterile (with aub^sting-3a)

(Schmidt et al., 1999)

male sterile | recessive

(Schmidt et al., 1999)

viable

(Bozzetti et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

aub^sting-1 homozygotes are viable.

aub^sting-1 homozygotes, but not aub^sting-1 heterozygotes, display a significant decrease in the length of third instar larval neuromuscular junctions, as compared to controls.

aub^sting-1 homozygotes, and aub^sting-1/aub^HN2 and aub^sting-1/aub^QC42 transheterozygotes exhibit crystalline aggregates (of Stellate protein) in spermatocytes, which are absent in aub^sting-1 single heterozygotes and wild-type controls.

(Bozzetti et al., 2015)

aub^sting-1 males have needle-shaped crystals in the spermatocytes in the presence of the Ste⁺ allele.

(Tritto et al., 2003)

Progeny from homozygous males are sometimes seen; male fertility in aub^sting-1/Y males is inversely correlated with Ste repeat copy number. Needle-shaped crystals are seen in the spermatocytes and spermatids of homozygous males. aub^sting-1/Y males show meiotic nondisjunction, and the frequency is directly correlated with Ste repeat copy number. aub^sting-1/aub^sting-3a flies are male sterile and produce needles in the spermatogenic stages.

(Schmidt et al., 1999)

male-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

aub^sting-1/aub[+] is a suppressor of sterile phenotype of Fmr1^Δ50M/Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of partially lethal - majority die | recessive phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of male semi-fertile phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of female semi-fertile phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of abnormal size | recessive | third instar larval stage phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of abnormal neuroanatomy | recessive | third instar larval stage phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

aub^sting-1/aub[+] is a suppressor of spermatocyte | spermatogenesis phenotype of AGO1^k08121

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ50M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of spermatocyte | spermatogenesis phenotype of AGO1⁰⁴⁸⁴⁵

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^sting-1/aub[+] is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

Other

Statement

Reference

Ste^unspecified, aub^sting-1 has spermatocyte phenotype

(Tritto et al., 2003, Schmidt et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

aub^sting-1 heterozygosity suppresses the following defects associated with Fmr1^Δ113M : the near lethality of Fmr1^Δ113M homozygotes; the sterility of Fmr1^Δ50M/Fmr1^Δ113M transheterozygous males and the fertility defects of Fmr1^Δ113M heterozygous or homozygous males and females; the crystalline aggregates observed in the spermatocytes of Fmr1^Δ113M heterozygotes and homozygotes; and the increased length of third instar larval neuromuscular junctions of Fmr1^Δ113M homozygotes. aub^sting-1 heterozygosity also suppresses the crystalline aggregates observed in the spermatocytes of AGO1⁰⁴⁸⁴⁵ heterozygotes, AGO1[k08121] heterozygotes, and Fmr1^Δ50M homozygotes.

aub^sting-1, AGO1^k08121 double homozygotes do not display significant changes in the length of third instar larval neuromuscular junctions, as compared to controls.

(Bozzetti et al., 2015)

aub^sting-1 males have star-shaped crystals in the spermatocytes in the presence of the Ste^unspecified allele.

(Tritto et al., 2003)

The crystals in primary spermatocytes of aub^sting-1 homozygous males carrying Ste^unspecified are star-shaped.

(Schmidt et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aub^sting-1 is rescued by aub^tMR-3

(Schmidt et al., 1999)

aub^sting-1 is rescued by aub^tMR-1

(Schmidt et al., 1999)

aub^sting-1 is rescued by aub^tMR-2

(Schmidt et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} element can cause reversion to wild-type.

(Schmidt et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

aub^sting-1

aub^sting

(Sahin et al., 2016, Bozzetti et al., 2015, Tritto et al., 2003)

ms(2)32D¹

sti

(Schmidt et al., 1999)

sting

(Tritto et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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