FlyBase Allele Report: Dmel\pb[5]

General Information

Symbol

Dmel\pb⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0013509

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Cribbs et al., 1995, Kapoun and Kaufman, 1995, Kapoun and Kaufman, 1995, Cribbs et al., 1992, Randazzo et al., 1991)

loss of function allele

(Benassayag et al., 1997)

Mutagen

gamma ray

(Kapoun and Kaufman, 1995)

Progenitor genotype

Cytology

Polytene chromosomes normal.

Description

Deletion of nucleotides G1099 through A1103 (coordinates of the lesion are the position in the 2-μ-4a spliced form of pb). This results in a frameshift at amino acid residue 368, producing a stop codon at residue 398.

(Tayyab et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:6,710,641..6,710,645 [-]

Comment:

Deletion of 5 nucleotides G1099-A1103 (with respect to pb-RA) causes a frameshift and early translation termination.

(Tayyab et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Johnston et al., 1998)

visible

(Kapoun and Kaufman, 1995, Kaufman et al., 1980)

visible (with pb²⁰)

(Tayyab et al., 2004)

visible | homeotic | somatic clone

(Joulia et al., 2005)

visible | recessive

(Boube et al., 1998)

Phenotype Manifest In

arista | ectopic (with pb⁴)

(Calgaro et al., 2002, Boube et al., 2000)

labellar cap (with pb²⁰)

(Tayyab et al., 2004)

labellum

(Boube et al., 1998, Johnston et al., 1998, Boube et al., 1997, Kapoun and Kaufman, 1995, Kapoun and Kaufman, 1995, Cribbs et al., 1992, Kaufman et al., 1980)

labellum (with pb⁴)

(Boube et al., 1997)

labellum | distal

(Boube et al., 1998)

labellum | distal (with pb⁴)

(Calgaro et al., 2002)

labellum | somatic clone

(Joulia et al., 2005)

labial disc

(Kapoun and Kaufman, 1995)

labial disc | somatic clone

(Joulia et al., 2005)

labial segment (with pb⁴)

(Boube et al., 2000)

leg | ectopic | somatic clone

(Joulia et al., 2005)

maxillary palp

(Johnston et al., 1998, Kapoun and Kaufman, 1995, Kapoun and Kaufman, 1995)

maxillary palp (with pb²⁰)

(Tayyab et al., 2004)

pseudotrachea (with pb⁴)

(Boube et al., 2000)

pseudotrachea (with pb²⁰)

(Tayyab et al., 2004)

tarsal segment | ectopic (with pb²⁰)

(Tayyab et al., 2004)

Detailed Description

Statement

Reference

pb⁵ clones, induced during the first and second instar larval stages using the FLP/FRT technique, cause some cells in the labial disc to adopt T1 leg fate. The mutant labial discs have a wild-type appearance during the early part of the larval third instar stage, but are at least two-fold larger than wild-type discs. The discs begin to show leg-like form by the end of this larval stage. Resulting adult labia may exhibit a distal leg claw, or sex comb teeth, typical of a T1 leg. pb⁵ clones can be obtained at a high frequency in the labial disc by their specific induction with Scer\FLP1^Scer\UAS.cDa under the control of Scer\GAL4^pb.PJ, in a Minute background to enhance mutant cell growth. The resulting mutant adults show a complete labial-to-T1 leg transformation.

(Joulia et al., 2005)

pb⁵/pb²⁰ flies have vestigial maxillary palps, the labellar caps are fully transformed to tarsi and the pseudotracheae are missing.

(Tayyab et al., 2004)

pb⁴/pb⁵ flies show a transformation of distal labium to antennal arista.

(Calgaro et al., 2002)

pb⁴/pb⁵ flies have a transformation of the distal labium to antennal arista, with a concomitant reduction of the pseudotrachea.

(Boube et al., 2000)

pb⁵/pb¹³ flies show a slight distal transformation of labium to antennal arista. If the flies are also heterozygous for N^55e11 the number of pseudotracheal rows is reduced and a region of apparent ectoderm forms between the remaining pseudotracheal rows and the transformed antennal structures. Homozygotes show replacement of labial palps by distal prothoracic legs.

(Boube et al., 1998)

pb¹/pb⁵ at 24^oC show distal labia transformed to a mixture of antenna and distal prothoracic legs. Of these 46% include claws and some display defined tarsal segments. These are never seen in any ct; pb/+ combinations. Maxillary palps of pb¹/pb⁵ are reduced in shape and ovoid, suggesting antennal transformation. This phenotype is not seen in ct; pb/+ combinations.

(Johnston et al., 1998)

Homozygotes show transformation of the labium to prothoracic leg structures. pb⁴/pb⁵ flies show mixed transformation of the distal labium to leg and aristal structures.

(Boube et al., 1997)

Labial palps are transformed into first thoracic legs and maxillary palps are reduced in size. Labial discs resemble leg discs.

(Kapoun and Kaufman, 1995)

Labial palps are transformed to first thoracic legs and maxillary palps are reduced in size.

(Kapoun and Kaufman, 1995)

The labial palp is transformed to prothoracic leg.

(Cribbs et al., 1992)

Flies of the genotype pb^+mRa/pb^+mRa; pb⁵/pb⁵ are completely rescued in terms of homeotic transformation.

(Randazzo et al., 1991)

Labial palps are transformed entirely to leg.

(Kaufman et al., 1980)

leg

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

pb⁵ has visible | homeotic | somatic clone phenotype, suppressible by hh^ts2

(Joulia et al., 2005)

pb⁵ has visible | homeotic | somatic clone phenotype, suppressible by hh^AC

(Joulia et al., 2005)

Other

Statement

Reference

pb⁵, put^p1 has visible | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, pygo¹³⁰ has visible | somatic clone phenotype

(Joulia et al., 2006)

Scr⁴/Scr⁵, pb⁵/pb¹ has visible | homeotic phenotype

(Abzhanov et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

pb⁵/pb⁴ has labial segment phenotype, enhanceable by skd^pap-1

(Boube et al., 2000)

pb⁵/pb⁴ has pseudotrachea phenotype, enhanceable by skd^pap-1

(Boube et al., 2000)

pb⁵/pb⁴ has labial segment phenotype, enhanceable by skd^pap-1/MED17^s2956

(Boube et al., 2000)

pb⁵/pb⁴ has pseudotrachea phenotype, enhanceable by skd^pap-1/MED17^s2956

(Boube et al., 2000)

pb⁵/pb⁴ has labellum phenotype, enhanceable by Df(3R)ΔC40b

(Boube et al., 1997)

Suppressed by

Statement

Reference

pb⁵ has labellum | somatic clone phenotype, suppressible by hh^AC

(Joulia et al., 2005)

pb⁵ has labellum | somatic clone phenotype, suppressible by hh^ts2

(Joulia et al., 2005)

pb⁵ has leg | ectopic | somatic clone phenotype, suppressible by hh^AC

(Joulia et al., 2005)

pb⁵ has leg | ectopic | somatic clone phenotype, suppressible by hh^ts2

(Joulia et al., 2005)

Enhancer of

Statement

Reference

pb⁵/pb[+] is an enhancer of pseudotrachea phenotype of Scer\GAL4^pb.PJ, ci^UAS.cAa

(Joulia et al., 2005)

Other

Statement

Reference

pb⁵, put^p1 has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, put^p1 has antenna | ectopic | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, put^p1 has labellum | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, pygo¹³⁰ has leg | ectopic | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, pygo¹³⁰ has labellum | somatic clone phenotype

(Joulia et al., 2006)

pb⁵, pygo¹³⁰ has bract | ectopic | somatic clone phenotype

(Joulia et al., 2006)

Scr⁴/Scr⁵, pb⁵/pb¹ has proboscis phenotype

(Abzhanov et al., 2001)

Scr⁴/Scr⁵, pb⁵/pb¹ has antenna | ectopic phenotype

(Abzhanov et al., 2001)

Scr⁴/Scr⁵, pb⁵/pb¹ has arista | ectopic phenotype

(Abzhanov et al., 2001)

Scr⁴/Scr⁵, pb⁵/pb¹ has third segment of antenna | ectopic phenotype

(Abzhanov et al., 2001)

Scr⁴/Scr⁵, pb⁵/pb¹ has second segment of antenna | ectopic phenotype

(Abzhanov et al., 2001)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has sex comb | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has unguis | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has sex comb | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has unguis | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

pb⁵/pb⁴, skd^pap-1 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has sex comb | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has unguis | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-1 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has tarsal segment | ectopic phenotype

(Boube et al., 2000)

MED17^s2956, pb⁵/pb⁴, skd^pap-53 has prothoracic leg | ectopic phenotype

(Boube et al., 2000)

ct^L188, pb⁵ has pseudotrachea phenotype

(Johnston et al., 1998)

ct^L188, pb⁵ has labellum phenotype

(Johnston et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

pygo¹³⁰ pb⁵ clones in the labium develop as diminished stubs with residual leg identity (as shown by the presence of bracted bristles).

put^p1 pb⁵ clones in the labium give rise to an equal proportion of T1 legs and antennal aristae (both transformations can occur in the same animal if both labial discs contain a clone).

put^p1 pb⁵ pygo¹³⁰ clones in the labium give rise to leg structures resembling that seen in pb⁵ pygo¹³⁰ clones.

(Joulia et al., 2006)

When mitotic clones induced in the labial disc are double mutant for both pb⁵ and hh^AC, the labial-to-leg transformation is modified. Overall tissue size is reduced compared to pb⁵ single mutants and distinct leg tarsi can no longer be seen, although remaining tissue still has prothoracic leg identity, as evidenced by the presence of sex comb teeth. When mitotic clones induced in the labial disc are double mutant for pb⁵ and hh^ts2 and larvae are raised at the restrictive temperature for the hh^ts2 mutation throughout the L3 stage, no labial-to-leg transformation occurs. Expression of ci^Scer\UAS.cAa under the control of Scer\GAL4^pb.PJ in pb⁵/+ heterozygotes enhances the reduced pseudotracheal row phenotype of ci^Scer\UAS.cAa single mutants.

(Joulia et al., 2005)

pb¹ Scr⁵/pb⁵ Scr⁴ flies show transformation of the proboscis into the antenna. The transformation varies from thick and shortened aristae with claws and pulvillae to apparently complete antennae with aristae, third and second antennal segments bearing characteristic bristles.

(Abzhanov et al., 2001)

The addition of a combination of skd^pap-1 and MED17^s2956 enhances the transformation phenotype seen in the labium of pb⁴/pb⁵ flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including sex comb teeth and claws) is seen. The addition of skd^pap-1 enhances the transformation phenotype seen in the labium of pb⁴/pb⁵ flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including claws) is seen.

(Boube et al., 2000)

pb⁴/pb⁵ flies also carrying one copy of Df(3R)ΔC40b generally have a more severe labial palp mutant phenotype, including the appearance of prothorax-specific sex comb teeth and distal claws.

(Boube et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pb⁵ is rescued by pb^ΔSalKpn

(Kapoun and Kaufman, 1995)

pb⁵ is rescued by pb^ΔPst

(Kapoun and Kaufman, 1995)

Partially rescued by

pb⁵ is partially rescued by pb^hs.PB

(Benassayag et al., 1997)

pb⁵ is partially rescued by pb^hs.PB

(Cribbs et al., 1995)

pb⁵ is partially rescued by pb^ΔKpn

(Kapoun and Kaufman, 1995)

pb⁵ is partially rescued by pb^ΔSal

(Kapoun and Kaufman, 1995)

Not rescued by

pb⁵ is not rescued by pb^Rev3.HSPB

(Benassayag et al., 1997)

Comments

Mutant leg transformation is incompletely rescued by pb^hs.PB, often resulting in a weak transformation of labium to antenna rather than to prothoracic leg. Alternatively, partially rescued flies may show small patches of labial tissue transformed to distal leg tissue.

(Cribbs et al., 1995)

Labial palp phenotype can be rescued by two copies of P{ΔSalKpn} and P{ΔPst} constructs.

(Kapoun and Kaufman, 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2169

Ki¹ pb⁵ red¹ e¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Duncan and Kaufman.

Induced on: Ki¹ red¹ e¹.

(Johnston et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

(Cribbs et al., 1992)

pb5

(Jordan et al., 2002)

pb⁵

(Enriquez et al., 2015, Kuert et al., 2014, Joulia et al., 2006, Yasunaga et al., 2006, Tayyab et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (21)

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