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General Information
Symbol
Dmel\pb5
Species
D. melanogaster
Name
FlyBase ID
FBal0013509
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Deletion of 5 nucleotides G1099-A1103 (with respect to pb-RA) causes a frameshift and early translation termination.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Deletion of nucleotides G1099 through A1103 (coordinates of the lesion are the position in the 2-μ-4a spliced form of pb). This results in a frameshift at amino acid residue 368, producing a stop codon at residue 398.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

pb5 clones, induced during the first and second instar larval stages using the FLP/FRT technique, cause some cells in the labial disc to adopt T1 leg fate. The mutant labial discs have a wild-type appearance during the early part of the larval third instar stage, but are at least two-fold larger than wild-type discs. The discs begin to show leg-like form by the end of this larval stage. Resulting adult labia may exhibit a distal leg claw, or sex comb teeth, typical of a T1 leg. pb5 clones can be obtained at a high frequency in the labial disc by their specific induction with Scer\FLP1Scer\UAS.cDa under the control of Scer\GAL4pb.PJ, in a Minute background to enhance mutant cell growth. The resulting mutant adults show a complete labial-to-T1 leg transformation.

pb5/pb20 flies have vestigial maxillary palps, the labellar caps are fully transformed to tarsi and the pseudotracheae are missing.

pb4/pb5 flies show a transformation of distal labium to antennal arista.

pb4/pb5 flies have a transformation of the distal labium to antennal arista, with a concomitant reduction of the pseudotrachea.

pb5/pb13 flies show a slight distal transformation of labium to antennal arista. If the flies are also heterozygous for N55e11 the number of pseudotracheal rows is reduced and a region of apparent ectoderm forms between the remaining pseudotracheal rows and the transformed antennal structures. Homozygotes show replacement of labial palps by distal prothoracic legs.

pb1/pb5 at 24oC show distal labia transformed to a mixture of antenna and distal prothoracic legs. Of these 46% include claws and some display defined tarsal segments. These are never seen in any ct; pb/+ combinations. Maxillary palps of pb1/pb5 are reduced in shape and ovoid, suggesting antennal transformation. This phenotype is not seen in ct; pb/+ combinations.

Homozygotes show transformation of the labium to prothoracic leg structures. pb4/pb5 flies show mixed transformation of the distal labium to leg and aristal structures.

Labial palps are transformed into first thoracic legs and maxillary palps are reduced in size. Labial discs resemble leg discs.

Labial palps are transformed to first thoracic legs and maxillary palps are reduced in size.

The labial palp is transformed to prothoracic leg.

Flies of the genotype pb+mRa/pb+mRa; pb5/pb5 are completely rescued in terms of homeotic transformation.

Labial palps are transformed entirely to leg.

leg

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

pb5 has visible | homeotic | somatic clone phenotype, suppressible by hhAC

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

pb5/pb4 has labial segment phenotype, enhanceable by skdpap-1

pb5/pb4 has pseudotrachea phenotype, enhanceable by skdpap-1

pb5/pb4 has labellum phenotype, enhanceable by Df(3R)ΔC40b

Suppressed by
Statement
Reference

pb5 has leg | ectopic | somatic clone phenotype, suppressible | heat sensitive by hhts2

pb5 has labellum | somatic clone phenotype, suppressible by hhAC

pb5 has leg | ectopic | somatic clone phenotype, suppressible by hhAC

Enhancer of
Statement
Reference

pb5/pb[+] is an enhancer of pseudotrachea phenotype of Scer\GAL4pb.PJ, ciUAS.cAa

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

pygo130 pb5 clones in the labium develop as diminished stubs with residual leg identity (as shown by the presence of bracted bristles).

putp1 pb5 clones in the labium give rise to an equal proportion of T1 legs and antennal aristae (both transformations can occur in the same animal if both labial discs contain a clone).

putp1 pb5 pygo130 clones in the labium give rise to leg structures resembling that seen in pb5 pygo130 clones.

When mitotic clones induced in the labial disc are double mutant for both pb5 and hhAC, the labial-to-leg transformation is modified. Overall tissue size is reduced compared to pb5 single mutants and distinct leg tarsi can no longer be seen, although remaining tissue still has prothoracic leg identity, as evidenced by the presence of sex comb teeth. When mitotic clones induced in the labial disc are double mutant for pb5 and hhts2 and larvae are raised at the restrictive temperature for the hhts2 mutation throughout the L3 stage, no labial-to-leg transformation occurs. Expression of ciScer\UAS.cAa under the control of Scer\GAL4pb.PJ in pb5/+ heterozygotes enhances the reduced pseudotracheal row phenotype of ciScer\UAS.cAa single mutants.

pb1 Scr5/pb5 Scr4 flies show transformation of the proboscis into the antenna. The transformation varies from thick and shortened aristae with claws and pulvillae to apparently complete antennae with aristae, third and second antennal segments bearing characteristic bristles.

The addition of a combination of skdpap-1 and MED17s2956 enhances the transformation phenotype seen in the labium of pb4/pb5 flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including sex comb teeth and claws) is seen. The addition of skdpap-1 enhances the transformation phenotype seen in the labium of pb4/pb5 flies. Instead of transformation to antennal structures, a transformation to T1 leg structures (including claws) is seen.

pb4/pb5 flies also carrying one copy of Df(3R)ΔC40b generally have a more severe labial palp mutant phenotype, including the appearance of prothorax-specific sex comb teeth and distal claws.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

pb5 is partially rescued by pbhs.PB

pb5 is partially rescued by pbhs.PB

pb5 is partially rescued by pbΔKpn

pb5 is partially rescued by pbΔSal

Not rescued by
Comments

Mutant leg transformation is incompletely rescued by pbhs.PB, often resulting in a weak transformation of labium to antenna rather than to prothoracic leg. Alternatively, partially rescued flies may show small patches of labial tissue transformed to distal leg tissue.

Labial palp phenotype can be rescued by two copies of P{ΔSalKpn} and P{ΔPst} constructs.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Duncan and Kaufman.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (19)