FlyBase Allele Report: Dmel\rdgB[2]

General Information

Symbol

Dmel\rdgB²

Species

D. melanogaster

Name

FlyBase ID

FBal0014479

Feature type

allele

Associated gene

Dmel\rdgB

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Milligan et al., 1997)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | conditional

(Lee et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

rdgB² mutant adults display progressive degeneration phenotype of rhabdomeres in peripheral photoreceptors, which are completely lost by day 5 post-eclosion, whereas the central UV-sensitive photoreceptor is spared.

(Yadav et al., 2015)

rdgB²/rdgB² flies show progressive retinal degeneration upon exposure to constant light.

(Lee et al., 2013)

Flies are defective in electroretinogram light response maintenance and also in the dark recovery. 7 day old flies show massive photoreceptor cell loss and ommatidial disorganisation.

(Lu et al., 1999)

Adult flies undergo retinal degeneration, characterised by dark-satining cell bodies and loss of rhabdomeres. Photoreceptor cells R1-R6 are more severely affected than photoreceptor cell R7, and degenerate before R7. The electroretinogram light response is abolished by saturating light treatment.

(Chang et al., 1997)

The electroretinogram (ERG) light response of 1 day old homozygous flies raised in a 12 hour light/dark cycle has a very small amplitude. 6 day old flies show massive retinal degeneration including reduced or absent rhabdomeres and holes in the retina. At older ages the R7 rhabdomere and photoreceptor also degenerate. Newly eclosed rdgB² flies show a wild-type ERG light response amplitude, but are defective in terminating the ERG light response. In addition, these flies also show prolonged dark recovery times compared to wild-type.

(Milligan et al., 1997)

Rhabdomeres and photoreceptor cell bodies degenerate gradually after eclosion. Retinal degeneration starts with the disappearance of SRC. Ocellar photoreceptors also degenerate and degeneration of the SRC is observed.

(Yoon et al., 1996)

Retinas of norpA³⁶; rdgB² mutants fail to degenerate in the dark or light, rdgB²; P{DG_q1-203} retinas degenerate in the dark and norpA³⁶; rdgB²; P{DG_q1-203} triple mutant retinas fail to degenerate in the light or dark.

(Lee et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

rdgB² has increased cell death | conditional phenotype, non-suppressible by Osi21^die4/Osi21^die4

(Lee et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

rdgB² has retina phenotype, suppressible by su(rdgA)40⁴⁰

(Georgiev et al., 2012)

rdgB² has photoreceptor neuron phenotype, suppressible by Mmus\Pitpnm2^ninaE.PL

(Lu et al., 1999)

rdgB² has ommatidium phenotype, suppressible by Mmus\Pitpnm2^ninaE.PL

(Lu et al., 1999)

rdgB² has retina phenotype, suppressible by stops⁶⁹

(Paetkau et al., 1999)

rdgB² has photoreceptor cell R1 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

rdgB² has photoreceptor cell R2 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

rdgB² has photoreceptor cell R3 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

rdgB² has photoreceptor cell R4 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

rdgB² has photoreceptor cell R5 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

rdgB² has photoreceptor cell R6 phenotype, suppressible by Mus\RdgB^ninaE.PC

(Chang et al., 1997)

NOT suppressed by

Statement

Reference

rdgB² has retina | conditional phenotype, non-suppressible by Osi21^die4/Osi21^die4

(Lee et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Osi21^die4/Osi21^die4 does not suppress the progressive retinal degeneration seen under constant light exposure in rdgB²/rdgB² flies.

(Lee et al., 2013)

The retinal degeneration phenotype of rdgB² flies is suppressed by su(rdgB)69⁶⁹; the time course of deep pseudopupil loss is slowed compared to rdgB² flies, but the electroretinogram light response of newly eclosed rdgB²; su(rdgB)69⁶⁹ flies is nearly identical to that of rdgB² flies.

(Paetkau et al., 1999)

The R1-R6 and electroretinogram phenotypes are completely rescued by Mus\RdgB^ninaE.PC.

(Chang et al., 1997)

The rdgB² phenotype is not rescued by Rnor\PITPNA^ninaE.PM. rdgB::Rnor\PITPNA^ninaE.PM fails to rescue rdgB², and may cause additional adverse effects; the initial ERG light response of rdgB² flies carrying rdgB::Rnor\PITPNA^ninaE.PM has a significantly smaller amplitude than that of rdgB² flies.

(Milligan et al., 1997)

Xenogenetic Interactions

Statement

Reference

The rdgB² electroretinogram phenotype is partially rescued by Mmus\RdgB2^ninaE.PL. 8 day old rdgB² flies carrying Mmus\RdgB2^ninaE.PL have slightly smaller rhabdomeres than normal and show some ommatidial disorganisation. 14 day old rdgB² flies carrying Mmus\RdgB2^ninaE.PL begin to show the rdgB² retinal degeneration phenotype.

(Lu et al., 1999)

Complementation and Rescue Data

Partially rescued by

rdgB² is partially rescued by rdgB^T59E.ninaE

(Milligan et al., 1997)

rdgB² is partially rescued by rdgB^{PITP.T59E.ninaE}

(Milligan et al., 1997)

rdgB² is partially rescued by rdgB^ninaE.PM

(Milligan et al., 1997)

rdgB² is partially rescued by rdgB^PITP.ninaE

(Milligan et al., 1997)

Not rescued by

rdgB² is not rescued by rdgB^T59A.ninaE

(Milligan et al., 1997)

Comments

6 day old and 30 day old rdgB² flies carrying rdgB^ninaE.PM have a wild-type ERG light response and show little evidence of retinal degeneration, showing the loss of the R7 and R8 rhabdomeres in some ommatidia. 17 day old rdgB² flies carrying rdgB^PITP.ninaE have a wild-type ERG light response and show little evidence of retinal degeneration, except for the loss of R7 cells. Both the ERG light response termination and dark recovery time phenotypes are substantially, but not completely, rescued if the flies are carrying either rdgB^T59E.ninaE or rdgB^{PITP.T59E.ninaE}. 6 day old rdgB² flies carrying either rdgB^T59E.ninaE or rdgB^{PITP.T59E.ninaE} have small or missing rhabdomeres and holes in the retina. rdgB² ; rdgB^T59E.ninaE flies have a more severe phenotype than rdgB² flies.

(Milligan et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Benzer.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rdgB2

(Lu et al., 1999)

rdgB²

(Trivedi et al., 2020, Yadav et al., 2018, Chen et al., 2015, Hardie et al., 2015, Yadav et al., 2015, Lee et al., 2013, Georgiev et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (18)

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