FlyBase Allele Report: Dmel\shi[4]

General Information

Symbol

Dmel\shi⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0015613

Feature type

allele

Associated gene

Dmel\shi

Associated Insertion(s)

Carried in Construct

Also Known As

shi^ts4

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Kim and Wu, 1990)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C to T. Amino acid replacement: P171S. P171S lies in the GTPase domain.

(Grant et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:15,895,125..15,895,125 [+]

Nucleotide change:

C15895125T

Reported nucleotide change:

C563T

Amino acid change:

Reported amino acid change:

P171S

(Grant et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

paralytic | heat sensitive

(Rikhy et al., 2003, Stimson et al., 2001, Krishnan et al., 1996, Chen and Stark, 1993)

paralytic | recessive | heat sensitive

(Kim and Wu, 1990)

paralytic | temperature conditional

(Krishnan et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant flies are paralysed at 28.5^oC.

(Krishnan et al., 2001)

Mutant flies show tight and complete paralysis in 2 minutes at 29^oC.

(Stimson et al., 2001)

Sub-anaesthetic concentration of carbon dioxide specifically suppresses the temperature sensitive paralytic phenotype. Sub-anaesthetic concentration of carbon dioxide also rapidly reverses paralysis induced at the restrictive temperature. The effect depends on the absolute concentration of CO₂ rather than the ratio with oxygen.

(Krishnan et al., 1996)

Hemizygous flies do not survive. shi²/shi⁴ flies become paralysed very slowly at 29^oC or 27^oC compared to shi² or shi⁴ homozygotes.

(Kim and Wu, 1990)

temperature-sensitive temperature of adult paralysis: 29^oC temperature causing developmental defects: none viability of allele over deficiency: lethal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

shi⁴ has paralytic | heat sensitive phenotype, enhanceable by sesB^org

(Rikhy et al., 2003)

shi⁴ has paralytic | heat sensitive phenotype, enhanceable by awd^MSF15

(Chen et al., 2002)

shi⁴ has paralytic | heat sensitive phenotype, enhanceable by stnB⁶/stnA⁶

(Stimson et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

shi⁴ is an enhancer of wing vein | ectopic phenotype of Cbl^Dv.UAS, Scer\GAL4^GMR.PF

(Robertson et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

sesB^org lowers the temperature at which 100% of shi⁴ flies are paralysed in 3 minutes, from 29^oC to 27^oC.

(Rikhy et al., 2003)

awd^MSF15 reduces the temperature threshold for paralysis by 6^oC in shi⁴ mutant flies.

(Chen et al., 2002)

shi⁴ stnA⁶ stnB⁶ flies undergo complete paralysis in 2 minutes at 26^oC.

(Stimson et al., 2001)

Thirty-seven percent of shi⁴; Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS wings exhibit an ectopic vein phenotype, moreso than in Scer\GAL4^GMR.PF Cbl^Dv.Scer\UAS mutants.

(Robertson et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Grigliatti.

Comments

Order of temperature sensitivity in the hemizygous condition: shi⁴ > shi²¹ > shi¹ > shi². Order of temperature sensitivity in the homozygous condition: shi²¹ = shi¹ > shi⁴ >= shi². Order of temperature sensitivity of heteroallelic combinations: shi²/shi²¹ = shi¹/shi²¹ >= shi⁴/shi²¹ > shi¹/shi² = shi¹/shi⁴ >> shi²/shi⁴.

(Kim and Wu, 1990)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shi⁴

shi^ts4

(Rikhy et al., 2007, Rikhy et al., 2003, Chen et al., 2002, Krishnan et al., 2001, Stimson et al., 2001, Robertson et al., 2000, Grant et al., 1998, Krishnan et al., 1996, Chen and Stark, 1993, Kim and Wu, 1990)

Name Synonyms

Secondary FlyBase IDs

References (11)

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