FlyBase Allele Report: Dmel\sesB[org]

General Information

Symbol

Dmel\sesB^org

Species

D. melanogaster

Name

orangi

FlyBase ID

FBal0154629

Feature type

allele

Associated gene

Dmel\sesB

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Rikhy et al., 2003)

Mutagen

ethyl methanesulfonate

(Rikhy et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: E266K. Nucleotide substitution: G?A.

(Rikhy et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,781,725..10,781,725 [-]

Nucleotide change:

G10781725A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

E266K

(Rikhy et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of mitochondrial metabolism disease

CEA

(Gokhale et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | heat sensitive

(Rikhy et al., 2003)

flightless (with Df(1)v-L15)

(Rikhy et al., 2003)

paralytic | heat sensitive (with Df(1)v-L15)

(Rikhy et al., 2003)

paralytic | heat sensitive (with sesB^G0386)

(Rikhy et al., 2003)

paralytic | recessive | heat sensitive

(Rikhy et al., 2003)

partially lethal - majority die (with Df(1)v-L15)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB¹)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^9Ed-1)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^9Ed-2)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^9Ed-3)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^9Ed-5)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^9Ed-6)

(Rikhy et al., 2003)

partially lethal - majority die (with sesB^G0386)

(Rikhy et al., 2003)

viable

(Rikhy et al., 2003)

viable (with sesB^9Ed-4)

(Rikhy et al., 2003)

Phenotype Manifest In

egg chamber | nutrition conditional

(DeVorkin et al., 2014)

Detailed Description

Statement

Reference

Ovaries from sesB^org flies exhibit reduced ATP levels compared with ovaries from wild-type flies. Fed sesB^org flies exhibit an increase in degenerating midstage egg chambers.

After amino acid deprivation, degenerating midstage egg chambers from sesB^org flies contain a reduction in the percentage of autolysosomes compared with controls. Non-degenerating midstage egg chambers from starved sesB^org flies also display reduced autophagic flux.

(DeVorkin et al., 2014)

100% of homozygotes are paralysed within 3 minutes at 37^oC. In addition, mutant flies paralyse if they are kept at lower temperatures for a longer duration. sesB^org is lethal in combination with Df(1)v-L15 at 25^oC. At 35^oC, development is slow, escapers are flightless and paralyse at 35^o. 90% of the expected number of sesB¹/sesB^org flies survive to adulthood. 36% of the expected number of sesB^9Ed-5/sesB^org flies survive to adulthood. 56% of the expected number of sesB^9Ed-2/sesB^org flies survive to adulthood. The flies show paralysis at 40-41^oC. 2% of the expected number of sesB^G0386/sesB^org or sesB^9Ed-1/sesB^org flies survive to adulthood. 1.6% of the expected number of sesB^9Ed-3/sesB^org flies survive to adulthood. 1.2% of the expected number of sesB^9Ed-6/sesB^org flies survive to adulthood. As the temperature is raised, the amplitude of all electroretinogram (ERG) components decreases in sesB^org flies; on- and off- transients as well as most of the total light response are abolished at the restrictive temperature. This defect is reversible if the flies are shifted back to the permissive temperature. Mutants show no substantial defect in biogenesis, stability or axonal transport of mitochondria in the motor terminals of the larval neuromuscular junction.

(Rikhy et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sesB^org has paralytic | heat sensitive phenotype, enhanceable by awd^MSM95

(Rikhy et al., 2003)

Enhancer of

Statement

Reference

sesB^org is an enhancer of paralytic | heat sensitive phenotype of shi^SHY

(Rikhy et al., 2003)

sesB^org is an enhancer of abnormal neurophysiology | heat sensitive phenotype of shi^CK2

(Rikhy et al., 2003)

sesB^org/sesB^org is an enhancer of paralytic | heat sensitive phenotype of shi^CK2

(Rikhy et al., 2003)

sesB^org is an enhancer of paralytic | heat sensitive phenotype of shi^BS1

(Rikhy et al., 2003)

sesB^org is an enhancer of paralytic | heat sensitive phenotype of shi²

(Rikhy et al., 2003)

sesB^org is an enhancer of paralytic | heat sensitive phenotype of shi⁴

(Rikhy et al., 2003)

NOT Enhancer of

Statement

Reference

sesB^org is a non-enhancer of paralytic | heat sensitive phenotype of shi¹

(Rikhy et al., 2003)

Suppressor of

Statement

Reference

sesB^org/sesB[+] is a suppressor of abnormal eye color phenotype of Hsap\BCL2L13^UAS.cNa, Scer\GAL4^GMR.PS

(Nakazawa et al., 2016)

NOT Suppressor of

Statement

Reference

sesB^org is a non-suppressor of paralytic | heat sensitive phenotype of shi¹

(Rikhy et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

sesB^org is a suppressor of egg chamber | nutrition conditional phenotype of Dcp-1^Prev1

(DeVorkin et al., 2014)

Other

Statement

Reference

Dcp-1^Prev1, sesB^org has nurse cell phenotype

(DeVorkin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Degenerating midstage egg chambers from nutrient-deprived sesB^org/+; Dcp-1^Prev1/Dcp-1^Prev1 flies contain persisting nurse cell nuclei and an accumulation of ref(2)P protein.

Ovarioles from fed sesB^org/sesB^org; Dcp-1^Prev1/Dcp-1^Prev1 flies arrested during mid-oogenesis, and these midstage egg chambers contain condensed and fragmented nurse cell nuclei and reduced ref(2)P levels.

(DeVorkin et al., 2014)

sesB^org lowers the temperature at which 100% of shi² flies are paralysed in 3 minutes, from 28^oC to 26^oC. sesB^org lowers the temperature at which 100% of shi⁴ flies are paralysed in 3 minutes, from 29^oC to 27^oC. sesB^org lowers the temperature at which 100% of shi^BS1 flies are paralysed in 3 minutes, from 32^oC to 30^oC. sesB^org lowers the temperature at which 100% of shi^SHY flies are paralysed in 3 minutes, from 35^oC to 33^oC. sesB^org lowers the temperature at which 100% of shi^CK2 flies are paralysed in 3 minutes, from 35^oC to 33^oC. 100% of sesB^org ; awd^MSM95/awd^MSM95 flies are paralysed in 3 minutes at 34^oC. 100% of sesB^org shi^CK2 ; awd^MSM95/awd^MSM95 flies are paralysed in 3 minutes at 27^oC. The on- and off-transients of the electroretinogram (ERG) are lost at 33^oC in sesB^org shi^CK2/Y flies. The on- and off-transients of the electroretinogram (ERG) are lost at 27^oC in sesB^org shi^CK2/Y ; awd^MSM95/awd^MSM95 flies.

(Rikhy et al., 2003)

Xenogenetic Interactions

Statement

Reference

The loss of pigment in the eye phenotype induced by expression of Hsap\BCL2L13^Scer\UAS.cNa driven by Scer\GAL4^GMR.PS is significantly suppressed by sesB^org/+. sesB^org/+ does not result in significant eye phenotypes in Scer\GAL4^GMR.PS>Hsap\BCL2L13^{ΔTM.Scer\UAS} flies.

(Nakazawa et al., 2016)

Complementation and Rescue Data

Fails to complement

sesB^G0386

(Rikhy et al., 2003)

Rescued by

sesB^org/sesB^G0386 is rescued by sesB^+t10.3

(Rikhy et al., 2003)

sesB^org is rescued by sesB^+t10.3

(Rikhy et al., 2003)

Comments

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