FlyBase Allele Report: Dmel\stg[4]

General Information

Symbol

Dmel\stg⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0016176

Feature type

allele

Associated gene

Dmel\stg

Associated Insertion(s)

Carried in Construct

Also Known As

stg^7M53, stg^7M

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Reuter et al., 1993)

Mutagen

ethyl methanesulfonate

(Mozer and Easwarachandran, 1999, Tearle et al., 1994, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Point mutant.

(Mozer and Easwarachandran, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Berger et al., 2010)

decreased cell death | embryonic stage

(Hartenstein and Posakony, 1990)

decreased occurrence of cell division | embryonic stage

(Hartenstein and Posakony, 1990)

lethal - all die during embryonic stage | recessive

(Mozer and Easwarachandran, 1999, Edgar and O'Farrell, 1989)

some die during embryonic stage | recessive

(Mozer and Easwarachandran, 1999, Edgar and O'Farrell, 1989)

Phenotype Manifest In

Detailed Description

Statement

Reference

The number of cells in the epithelium of the hindgut at stage 13 is significantly reduced compared to wild type in stg⁴/Df(3R)BSC500 embryos.

stg⁴/Df(3R)BSC500 stage 13 embryos show defects in the left-right asymmetry in their hindgut, with some embryos showing no asymmetry, and other showing inverse asymmetry.

(Nakamura et al., 2013)

stg⁴ mutant embryos exhibit mitotic arrest in cell division cycle 14.

(Berger et al., 2010)

The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.

(Milchanowski et al., 2004)

Eye of adults heterozygous with stg^hwy are normal, though missing macrochaetae phenotype remains.

(Mozer and Easwarachandran, 1999)

Transheterozygotes with stg^S114302b produce adult escapers that lack scutellars and many other bristles, have rough eyes and occasionally lack a haltere or have notched wings.

(Salzberg et al., 1997)

Lethal interaction with noose¹ and noose², and a semi-lethal interaction with noose³ and noose⁴. Transheterozygotes with stg^knot are viable and fertile.

(White-Cooper et al., 1996)

Primordial germ cells (PGCs) leave the posterior midgut (PMG) at the same time as in wild type and no significant differences in their migration to the gonadal mesoderm were observed.

(Jaglarz and Howard, 1995)

Heterozygotes with Dr^Mio and Dr¹ are semi-lethal, 10-30% adults survive to eclosion, surviving adults exhibit reduced eye, deranged bristle pattern on the thorax, abdomen, legs and wing margins and locomotor defects so the flies could hardly move.

(Tearle et al., 1994)

No cell division occurs in the ectoderm after the blastoderm stage in homozygotes. Cell death does not appear to occur in late embryos (stage 16) in contrast to wild-type.

(Hartenstein and Posakony, 1990)

The mesoderm is able to disperse and spread out normally after gastrulation in stg⁴ mutant embryos.

(Leptin and Grunewald, 1990)

Mutants show a nonspecific reduction in the number of differentiated cuticular structures.

(Edgar and O'Farrell, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

stg⁴ is a non-enhancer of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Suppressor of

Statement

Reference

wg^Sp-1, wg[+], stg⁴, stg[+] is a suppressor | partially of visible phenotype of mir-965^KO1/mir-965^KO2

(Verma and Cohen, 2015)

stg⁴/stg[+] is a suppressor | partially of visible phenotype of mir-965^KO1/mir-965^KO2

(Verma and Cohen, 2015)

stg⁴/stg[+] is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg⁴/stg⁴ is a suppressor of abnormal cytokinesis | embryonic stage phenotype of pbl³

(Schumacher et al., 2004)

stg⁴/stg[+] is a suppressor of abnormal mitotic cell cycle phenotype of Myb^ΔC.GMR

(Okada et al., 2002)

stg⁴/stg[+] is a suppressor of visible | dominant phenotype of Myb^ΔC.GMR

(Okada et al., 2002)

NOT Suppressor of

Statement

Reference

stg⁴ is a non-suppressor of visible phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

stg⁴ is an enhancer of wing margin bristle | ectopic phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Marygold and Vincent, 2003)

NOT Enhancer of

Statement

Reference

stg⁴ is a non-enhancer of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

stg⁴ is a non-enhancer of phenotype of rux⁹

(Gonczy et al., 1994)

Suppressor of

Statement

Reference

stg⁴/stg[+] is a suppressor | partially of abdomen phenotype of mir-965^KO1/mir-965^KO2

(Verma and Cohen, 2015)

wg^Sp-1, wg[+], stg⁴, stg[+] is a suppressor | partially of abdomen phenotype of mir-965^KO1/mir-965^KO2

(Verma and Cohen, 2015)

stg⁴/stg[+] is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg⁴/stg[+] is a suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

stg⁴/stg[+] is a suppressor of eye phenotype of Myb^ΔC.GMR

(Okada et al., 2002)

NOT Suppressor of

Statement

Reference

stg⁴ is a non-suppressor of eye phenotype of upd1^GMR.PB

(Mukherjee et al., 2006)

stg⁴/stg⁴ is a non-suppressor of mesoderm phenotype of pbl³

(Schumacher et al., 2004)

stg⁴ is a non-suppressor of phenotype of rux⁹

(Gonczy et al., 1994)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of stg⁴ partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies. The asynchronous histoblast division and reduction in histoblast migration rate are also suppressed.

One copy each of stg⁴ and wg^Sp-1 partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies.

(Verma and Cohen, 2015)

Cell cycle arrested NB6-4t cells in CycE^AR95;stg⁴ double mutants show glial identity.

(Berger et al., 2010)

Mesodermal cells in stumps^09904b stg⁴ double mutant embryos are able to disperse from the site of mesoderm invagination.

(Wilson et al., 2005)

In stg²/stg⁴,Df(3L)ri-79c double mutant embryos no post-blastoderm cell divisions occur and gastrulation is normal.

(Seher and Leptin, 2000)

Lowering the dose of stg or cdc2 has no effect on the germline phenotype of rux.

(Gonczy et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

stg^hwy

(Mozer and Easwarachandran, 1999)

Fails to complement

stg^Dr-L2

(Mozer, 2001)

stg^S024532

(Prokopenko et al., 2000)

stg^S114302b

(Salzberg et al., 1997)

Comments

Complements stg^hwy for ommatidial phenotype, fails to complement stg^hwy for bristle phenotype.

(Mozer and Easwarachandran, 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2500

ru¹ hry¹ Diap1^th-1 st¹ cu¹ sr¹ e^s stg⁴ ca¹/TM3, Sb¹ Ser¹

Kyoto

107367

ru¹ hry¹ Diap1^th-1 st¹ cu¹ sr¹ e^s stg⁴ ca¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Trichogen and tormogen cells are absent in homozygous stg⁴ embryos, as assayed by Ecol\lacZ^A1-2-29 expression.

(Hartenstein and Posakony, 1990)

stg is not required for endoreplication of polytene DNA in the larva.

(Smith and Orr-Weaver, 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

(Mukherjee et al., 2006)

Stg⁴

(McPherson et al., 2023, Glavic et al., 2009)

stg⁴

(Sun et al., 2024, Macabenta et al., 2022, Yaghmaeian Salmani et al., 2018, Weber and Mlodzik, 2017, Verma and Cohen, 2015, Wang and Baker, 2015, Baumgardt et al., 2014, Chang et al., 2013, Nakamura et al., 2013, Berger et al., 2010, Christensen et al., 2009.7.22, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, White et al., 2007)

stg^7M53

(Dey et al., 2025, Di Talia and Wieschaus, 2012, Clark et al., 2011, Grosskortenhaus et al., 2005, Wilson et al., 2005, Isshiki et al., 2001, Seher and Leptin, 2000, Yamaguchi et al., 1995, Reuter et al., 1993, Smith and Orr-Weaver, 1991, Hartenstein and Posakony, 1990, Smouse and Perrimon, 1990, Edgar and O'Farrell, 1989)

stg^7M

(Tanaka-Matakatsu et al., 2007, Schumacher et al., 2004, Marygold and Vincent, 2003, Mozer, 2001, White-Cooper et al., 1996, Jaglarz and Howard, 1995, Tearle et al., 1994, Leptin and Grunewald, 1990, Tearle and Nusslein-Volhard, 1987)

string7M53

(Sun and Stathopoulos, 2018)

Name Synonyms

Secondary FlyBase IDs

FBal0099823

References (48)

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