FlyBase Allele Report: Dmel\Tl[r4]

General Information

Symbol

Dmel\Tl^r4

Species

D. melanogaster

Name

FlyBase ID

FBal0016839

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

Also Known As

Toll^rm9, Tl^rm9, Toll^rm10, Tl^rm10, mel(3)9, mel(3)10

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Tearle and Nusslein-Volhard, 1987)

loss of function allele

(Schneider et al., 1991)

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992, Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Anderson et al., 1985)

Description

Nucleotide substitution: AA1639GC. Amino acid replacement: K355S. Nucleotide substitution: C2715A. Amino acid replacement: T714N. These mutations are in the extracellular domain of the protein.

(Schneider et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3R:26,838,800..26,838,801 [+]

Nucleotide change:

AAA26838799AGC

Reported nucleotide change:

AA1639GC

Amino acid change:

T356P | Tl-PB; T356P | Tl-PC; T356P | Tl-PD

Reported amino acid change:

K355S

Comment:

The reported Lys to Ser amino acid change (AAA to AGC) requires nucleotide changes at two positions of the codon. This is one of two amino acid substitutions in Tl in this strain. The site of the nucleotide substitution in the mutant was inferred by FlyBase based on the reported amino acid change.

(Schneider et al., 1991)

point_mutation

3R:26,839,994..26,839,994 [+]

Nucleotide change:

C26839994A

Reported nucleotide change:

C2715A

Amino acid change:

T714N | Tl-PB; T714N | Tl-PC; T714N | Tl-PD

Reported amino acid change:

T714N

Comment:

One of two amino acid substitutions in this strain.

(Schneider et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal vessel wall cell (with Tl^r3)

(Wang et al., 2005)

embryonic dorsal vessel (with Tl^r3)

(Wang et al., 2005)

Detailed Description

Statement

Reference

Tl^r3/Tl^r4 embryos display abnormal positioning of cardioblasts and gaps in the dorsal vessel.

(Wang et al., 2005)

The entire dorso-ventral axis forms neuroectoderm in homozygous Tl^r4 embryos.

(Cowden and Levine, 2003)

Cell intercalation in lateralized Tl mutant embryos proceeds normally during germ band extension.

(Irvine and Wieschaus, 1994)

Unique lateralized pattern. Cuticular phenotype of embryos resemble that of Toll-dominant embryos, but denticles finer and less heavily pigmented and body shape often an elongated tube. Recessive.

(Lindsley and Zimm, 1992)

Dorsalized phenotype facilitates removal of perivitelline fluid.

(Stein and Nusslein-Volhard, 1992)

Lateralized embryos: cuticle is an elongated tube covered with rings of denticles. dpp, zen and twi are only expressed at the poles, only transient expression of sna is seen.

(Ray et al., 1991)

Embryos from homozygous females are lateralised.

(Schneider et al., 1991)

Females produced lateralized embryos.

(Gerttula et al., 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Tl[+]/Tl^r4 is an enhancer of embryonic ventral epidermis | non-rescuable maternal effect | germline clone phenotype of wek^EX14

(Chen et al., 2006)

Tl[+]/Tl^r4 is an enhancer of embryonic/first instar larval cuticle | ventral | non-rescuable maternal effect | germline clone phenotype of wek^EX14

(Chen et al., 2006)

Other

Statement

Reference

Tl^r4, bcd⁷, cic¹, osk⁶, tsl¹ has embryo phenotype

(Soluri et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The variable dorsalized embryonic phenotype of wek^EX14 mutants is enhanced by Tl^r4. While 51% of wek^EX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 97% in wek^EX14; Tl^r4/+ embryos.

(Chen et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Tl^rK344

(Cook, 2000.10.9)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2507

ru¹ hry¹ Diap1^th-1 st¹ kni^ri-1 rn^roe-1 p^p e¹ Tl^r4/TM3, Ser¹

Notes on Origin

Discoverer

T. Rice.

(Lindsley and Zimm, 1992)

Comments

It is not known whether one or both amino acid replacements generate the mutant phenotype. Phenotypic and molecular analysis suggests that the allele previously termed "Tl^rm10" is the same mutagenic event as "Tl^rm9".

(Schneider et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Tl^RM9

(Soluri et al., 2020)

Tl^r4

(Shen et al., 2022, Iijima et al., 2020, Alpar et al., 2018, Liu et al., 2016, Stramer et al., 2008, Mizutani et al., 2006, Wang et al., 2005)

Tl^rm10

(Gomez et al., 2024, Hendrix et al., 2008, Cowden and Levine, 2003, Stathopoulos et al., 2002, Irvine and Wieschaus, 1994, Lindsley and Zimm, 1992, Schneider et al., 1991, Anderson et al., 1985)

Tl^rm9

(Gomez et al., 2024, Hendrix et al., 2008, Chasan and Anderson, 1993, Costa et al., 1993, Anderson et al., 1992, Lindsley and Zimm, 1992, Stein and Nusslein-Volhard, 1992, Ray et al., 1991, Roth et al., 1991, Schneider et al., 1991, Gerttula et al., 1988, Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985)

Toll^r4

(Chen et al., 2006)

Toll^rm10

(Hunt et al., 2024, Chopra et al., 2012, Garcia and Stathopoulos, 2011, Biemar et al., 2005, Cowden and Levine, 2002, Stathopoulos and Levine, 2002)

Toll^rm9

(Hunt et al., 2024, Chopra et al., 2012, Garcia and Stathopoulos, 2011, Biemar et al., 2005, Cowden and Levine, 2003, Cowden and Levine, 2002, Stathopoulos and Levine, 2002, Stathopoulos et al., 2002)

mel(3)10

(Anderson et al., 1985, Anderson and Nusslein-Volhard, 1984)

mel(3)9

(Lindsley and Zimm, 1992, Anderson et al., 1985, Anderson and Nusslein-Volhard, 1984)

rm10

(Riede, 2001)

rm9

(Riede, 2001)

Name Synonyms

Tollrm10

(Biemar et al., 2006)

Tollrm9

(Biemar et al., 2006)

Secondary FlyBase IDs

FBal0030326
FBal0034113

References (36)

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