FlyBase Allele Report: Dmel\osk[6]

General Information

Symbol

Dmel\osk⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0013308

Feature type

allele

Associated gene

Dmel\osk

Associated Insertion(s)

Carried in Construct

Also Known As

osk¹⁶⁶

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Irish et al., 1989)

loss of function allele

(Eldon and Pirrotta, 1991)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: R593W. Nucleotide substitution: C2506T.

(Kim-Ha et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:8,937,323..8,937,323 [+]

Nucleotide change:

C8937323T

Reported nucleotide change:

C2506T

Amino acid change:

R593W | osk-PA; R455W | osk-PC

Reported amino acid change:

R593W

(Kim-Ha et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

In osk⁶/osk^GM52 mutants, where three primordial germ cells are incorporated into the gonad (on average), the germ cell division rate is faster than in wild-type until the end of the second instar larval stage. However, the proliferation rate slows down as the number of primordial germ cells approaches wild-type numbers.

(Gilboa and Lehmann, 2006)

An increased number of cells undergo TUNEL-positive cell death in the posterior half of osk³/osk⁶ embryos compared to wild-type embryos. This cell death at the embryonic stage corresponds to missing tissue at the larval stage.

(Werz et al., 2005)

Embryos from osk⁹/osk⁶ mothers completely lack an abdomen.

(Yoshida et al., 2004)

Individuals carrying one or two copies of P{osk⁺108} have a wild type phenotype. Most individuals carrying one copy of P{oskBRE^-} have anterior pattern deletions, head structures and the first thoracic denticle belt are missing, two copies cause a more severe posteriorization of the body pattern phenotype.

(Kim-Ha et al., 1995)

Dvir\osk^virosk provides poterior body patterning to osk² and osk⁶ embryos, but in some instances the activity has spread towards the anterior of the embryo.

(Webster et al., 1994)

Strong abdominal defects.

(Ephrussi et al., 1991)

Absence of posterior pole plasm, polar granules and pole cells.

(Lehmann and Nusslein-Volhard, 1991)

Does not interact with RpII140^wimp maternal effect.

(Parkhurst and Ish-Horowicz, 1991)

Embryos lack pole cells. Large amounts of the protein encoded by vas are expressed in early stages of oogenesis. The protein encoded by vas fails to distribute asymmetrically. Cleavage embryos have uniform distribution of the protein encoded by vas, which disappears by early gastrulation.

(Lasko and Ashburner, 1990)

Hemizygous embryos derived from homozygous females have no polar granules, fail to form pole cells and have deletions of abdominal structures. Posterior localization of vas and CycB transcripts is completely abolished.

(Raff et al., 1990)

bcd protein distribution is the same as in wild type embryos.

(Driever and Nusslein-Volhard, 1988)

Mouth hooks or only prothoraces may be formed at both ends of the embryo in embryos derived from exu¹/exu¹; osk⁶/osk⁶ embryos.

(Frohnhofer and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

osk⁶/osk³ has increased cell death | embryonic stage phenotype, suppressible by BacA\p35^UASp.cWa/Scer\GAL4^Tub.PU

(Werz et al., 2005)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

osk⁶/osk⁹ has embryonic abdomen phenotype, non-enhanceable | maternal effect by Pka-R1¹⁸³⁰⁴/Df(3L)ME107

(Yoshida et al., 2004)

osk⁶/osk⁹ has embryonic/first instar larval cuticle phenotype, non-enhanceable | maternal effect by Pka-R1¹⁸³⁰⁴/Df(3L)ME107

(Yoshida et al., 2004)

Suppressed by

Statement

Reference

osk⁶ has embryo | maternal effect phenotype, suppressible by tsl^CBB.bcd/bcd⁶/tsl^PZRev32

(Stevens et al., 2003)

NOT suppressed by

Statement

Reference

osk⁶/osk⁹ has embryonic/first instar larval cuticle phenotype, non-suppressible | maternal effect by Pka-R1¹⁸³⁰⁴/Df(3L)ME107

(Yoshida et al., 2004)

osk⁶/osk⁹ has embryonic abdomen phenotype, non-suppressible | maternal effect by Pka-R1¹⁸³⁰⁴/Df(3L)ME107

(Yoshida et al., 2004)

Suppressor of

Statement

Reference

tsl^CBB.bcd, osk⁶, tsl^PZRev32 is a suppressor of embryo | maternal effect phenotype of bcd⁶

(Stevens et al., 2003)

Other

Statement

Reference

Tl^r4, bcd⁷, cic¹, osk⁶, tsl¹ has embryo phenotype

(Soluri et al., 2020)

bcd⁶, osk⁶, tsl^PZRev32 has embryo | maternal effect phenotype

(Stevens et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of BacA\p35^{Scer\UAS.P\T.cWa} under the control of Scer\GAL4^tub significantly reduces the amount of TUNEL-positive cell death in osk³/osk⁶ embryos.

(Werz et al., 2005)

The phenotype of embryos from osk⁹/osk⁶ mothers is unaffected by the mothers carrying Pka-R1¹⁸³⁰⁴/Df(3L)ME107.

(Yoshida et al., 2004)

Embryos produced by bcd⁶, osk⁶ and tsl^PZRev32 mutant mothers lack all anterior posterior patterning. This phenotype is partially rescued by the addition of tsl^CBB.bcd, leading to embryos that differentiate filzkorper material, at one or both poles.

(Stevens et al., 2003)

Injection of nos^N5 RNA into homozygous embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

osk⁶ is rescued by osk^+t8

(Kim-Ha et al., 1995)

Not rescued by

osk⁶ is not rescued by osk^ΔBRE

(Kim-Ha et al., 1995)

Comments

Mutant phenotype can be rescued by P element mediated transformation of a wild type gene copy.

(Kim-Ha et al., 1991)

Images (0)

Mutant

Wild-type

Stocks (6)

Bloomington

3252

st¹ bcd⁷ p^p osk⁶/TM3, Sb¹ Ser¹

3291

rn^roe-1 p^p bcd⁷ osk⁶ tsl¹/TM3, Ser¹

2987

Diap1^th-1 st¹ kni^ri-1 rn^roe-1 osk⁶ p^p/TM3, Sb¹ Ser¹

3259

st¹ kni^ri-1 rn^roe-1 p^p osk⁶ e¹ tsl⁴ ca¹/TM3, Sb¹

Kyoto

106940

st¹ bcd⁷ p^p osk⁶ / TM3, Sb¹ Ser¹

107509

Diap1^th-1 st¹ kni^ri-1 rn^roe-1 osk⁶ p^p/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Cytoplasm transplanted from osk⁶ mutant embryos does not rescue the abdominal phenotype of pum¹³ mutant embryos. Cytoplasm transplanted from pum¹³ mutant embryos rescues the abdominal phenotype of osk⁶ mutant embryos to the same extent as wild-type cytoplasm.

(Lehmann and Nusslein-Volhard, 1987)

hb protein expression in early osk⁶ mutant embryos has been studied.

(Tautz, 1988)

Normal localization of osk mRNA.

(Kim-Ha et al., 1991)

Although tud protein is present in mutant embryo extracts, its localization in the embryo is altered.

(Bardsley et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

osk¹¹⁶

(Akiyama and Okada, 1992)

osk¹⁶⁶

(Soluri et al., 2020, Gilboa and Lehmann, 2006, Werz et al., 2005, Cinnamon et al., 2004, Yoshida et al., 2004, Yohn et al., 2003, Ahmed et al., 2002, Zallen et al., 2002, Tsai et al., 1998, Chen et al., 1997, Greenwood and Struhl, 1997, Rongo et al., 1997, Dahanukar and Wharton, 1996, Smibert et al., 1996, Wolfgang and Forte, 1995, Ding et al., 1994, Bardsley et al., 1993, Klingler and Gergen, 1993, Akiyama and Okada, 1992, Gavis and Lehmann, 1992, Struhl et al., 1992, Bronner and Jäckle, 1991, Eldon and Pirrotta, 1991, Ephrussi et al., 1991, Jacob et al., 1991, Kim-Ha et al., 1991, Lehmann and Nusslein-Volhard, 1991, Parkhurst and Ish-Horowicz, 1991, Riddihough and Ish-Horowicz, 1991, Riley et al., 1991, St. Johnston et al., 1991, Hay et al., 1990, Hulskamp et al., 1990, Jack and McGinnis, 1990, Lasko and Ashburner, 1990, Raff et al., 1990, Casanova and Struhl, 1989, Hooper et al., 1989, Driever and Nusslein-Volhard, 1988, Lehmann, 1988, Tautz, 1988, Frohnhofer and Nusslein-Volhard, 1987, Lehmann and Nusslein-Volhard, 1987, Tearle and Nusslein-Volhard, 1987)

osk⁶

(Christensen et al., 2008.4.15, Hong et al., 2008)

oskar¹⁶⁶

(Wharton and Struhl, 1989)

oskar⁶

(Shimell et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (60)

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