FlyBase Allele Report: Dmel\tsl[3]

General Information

Symbol

Dmel\tsl³

Species

D. melanogaster

Name

FlyBase ID

FBal0017197

Feature type

allele

Associated gene

Dmel\tsl

Associated Insertion(s)

Carried in Construct

Also Known As

tsl¹⁴⁶

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Savant-Bhonsale and Montell, 1993, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Y279N.

(Savant-Bhonsale and Montell, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,778,579..21,778,579 [-]

Nucleotide change:

T21778579A

Amino acid change:

Y279N | tsl-PA; Y279N | tsl-PB; Y279N | tsl-PC

Reported amino acid change:

Y279N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Savant-Bhonsale and Montell, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

embryonic/larval segmentation phenotype (with tsl⁴)

(Pae et al., 2017)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

Phenotype Manifest In

abdominal segment 8 | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

embryonic abdominal segment 8

(Stevens et al., 1990)

embryonic head

(Stevens et al., 1990)

embryonic head (with tsl⁴)

(Pae et al., 2017)

embryonic head | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

embryonic telson

(Stevens et al., 1990)

embryonic telson (with tsl⁴)

(Pae et al., 2017)

filzkorper | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

labrum | embryonic stage

(Stevens et al., 1990)

primordial germ cell | embryonic stage (with tsl⁴)

(Pae et al., 2017)

telson | embryonic stage | maternal effect (with tsl^Δ)

(Johnson et al., 2017)

Detailed Description

Statement

Reference

Late embryos from tsl³/tsl^Δ transheterozygous mothers exhibit a fully penetrant "terminal class mutant" phenotype, in which some terminal structures are missing (including head structures, abdominal segment 8, telson, and filzkorper), as compared to controls.

(Johnson et al., 2017)

tsl³/tsl⁴ transheterozygotes present a pole-hole phenotype during early embryonic stage and present patterning defects at late embryonic stage, as shown by head and telson defects in cuticle preparations, as compared to controls.

(Pae et al., 2017)

Little or no tll expression is detected in the posterior of syncytial or cellular blastoderm embryos, at the anterior the early tll cap does not appear and an abnormal anterior tll stripe appears by the late syncytial blastoderm.

(Pignoni et al., 1992)

Embryos derived from homozygous females lack the labrum anteriorly, and the head is reduced in size. Posteriorly, A8 and the telson are deleted.

(Stevens et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

tsl⁴/tsl³ has embryonic/larval segmentation phenotype phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic/larval segmentation phenotype phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

Suppressor of

Statement

Reference

tsl⁴/tsl³ is a suppressor of decreased cell number | recessive | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

tsl[+]/tsl³ is a non-suppressor of decreased cell number | recessive | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Other

Statement

Reference

gcl^rev390, tsl⁴/tsl³ has increased cell number | embryonic stage phenotype

(Pae et al., 2017)

bcd⁶, nanos^L7, tsl³ has abnormal cell polarity | maternal effect | gastrula stage phenotype

(Zallen and Wieschaus, 2004)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

tsl⁴/tsl³ has embryonic head phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic telson phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype, non-suppressible by gcl^rev390/gcl[+]

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic head phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

tsl⁴/tsl³ has embryonic telson phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype, non-suppressible by gcl^rev390/gcl^rev390

(Pae et al., 2017)

Suppressor of

Statement

Reference

tsl⁴/tsl³ is a suppressor of germline cell | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

NOT Suppressor of

Statement

Reference

tsl[+]/tsl³ is a non-suppressor of germline cell | embryonic stage phenotype of gcl^rev390

(Pae et al., 2017)

Other

Statement

Reference

gcl^rev390, tsl⁴/tsl³ has primordial germ cell | embryonic stage phenotype

(Pae et al., 2017)

bcd⁶, nanos^L7, tsl³ has embryo | embryonic stage 4 phenotype

(Blankenship and Wieschaus, 2001)

bcd⁶, nanos^L7, tsl³ has epidermis phenotype

(Lehmann and Frohnhofer, 1989)

Additional Comments

Genetic Interactions

Statement

Reference

The pole-hole phenotype in early embryonic stage and the patterning defects in late embryonic stage (i.e. head and telson defects in cuticle preparations) displayed by tsl³/tsl⁴ transheterozygotes are not suppressed by gcl^rev390 heterozygosity or homozygosity.

The decreased primordial germ cells in gcl^rev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl³/tsl⁴ transheterozygosity, but not by tsl³ heterozygosity. tsl³/tsl⁴, gcl^rev390/+ and tsl³/tsl⁴, gcl^rev390/gcl^rev390 double mutants do not present any obvious defects in either centrosome positioning or cell division of primordial germ cells in the early embryo, as compared to controls.

(Pae et al., 2017)

Anisotropic epithelial constriction still occurs in bcd⁶ nos^L7 tsl³ triple mutant embryos that lack anterior-posterior polarity.

(Martin et al., 2010)

Anterior-posterior polarization of cells in the extending germband is disrupted in embryos from bcd⁶ nos^L7 tsl³ homozygous mothers.

(Zallen and Wieschaus, 2004)

Embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females have uniform yolk stalk diameters (in contrast to wild type where there are three domains of varying yolk stalk diameter along the anterior-posterior axis of the embryo). The shallow cellularisation front of the anterior domain and the greater depth of the pre-cephalic furrow domain are also lost. Other aspects of cellularisation are normal in these embryos. Nuclear spacing is uniform along the anterior-posterior axis in embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females (in contrast to wild type where there is an anterior domain of lower nuclear density). The larger diameter of the actin caps seen in the anterior of the wild-type embryo in cycle 11 and 12 is not seen in embryos derived from bcd⁶ nos^L7 tsl³ triple mutant females.

(Blankenship and Wieschaus, 2001)

bcd⁶ nos^L7 tsl³ embryos develop a cuticle but no segmental pattern. Injection of wild type posterior pole plasm induces formation of anterior abdominal segments towards the end of the embryo.

(Lehmann and Frohnhofer, 1989)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

3310

st¹ tsl³ tll¹/TM3, Sb¹

Notes on Origin

Discoverer

Comments

A strong allele of tsl.

(Pignoni et al., 1992)

Allelic series: tsl⁵ = tsl⁴ > tsl³ > tsl¹ > tsl²

(Savant-Bhonsale and Montell, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tsl¹⁴⁶

(Suresh et al., 2017, Zallen and Wieschaus, 2004, Blankenship and Wieschaus, 2001, Tsai et al., 1998, Van Doren et al., 1998, Irvine and Wieschaus, 1994, Savant-Bhonsale and Montell, 1993, Pignoni et al., 1992, Stevens et al., 1990, Lehmann and Frohnhofer, 1989, Tearle and Nusslein-Volhard, 1987)

tsl³

(Johnson et al., 2017, Pae et al., 2017)

tsl^Y279N

(Pae et al., 2017)

Name Synonyms

torso-like146

(Martin et al., 2010)

Secondary FlyBase IDs

References (16)

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