FlyBase Allele Report: Dmel\IP3K2[wy-2]

General Information

Symbol

Dmel\IP3K2^wy-2

Species

D. melanogaster

Name

FlyBase ID

FBal0018546

Feature type

allele

Associated gene

Dmel\IP3K2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Dean et al., 2015)

Mutagen

Progenitor genotype

Cytology

Description

5bp deletion in the open reading frame, downstream of the calmodulin binding site and catalytic domain. The frameshift caused by the deletion is predicted to result in a sequence of 6 changed amino acids followed by a premature stop codon.

(Dean et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:13,327,870..13,327,874 [+]

Comment:

A 5 base pair deletion causes a frameshift, followed by 6 novel amino acids and a stop codon. Extent of deletion from GB:KT732029.

(Dean et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Dean et al., 2015)

visible (with Df(1)Exel6245)

(Dean et al., 2015)

visible (with IP3K2^wy-1)

(Dean et al., 2015)

visible (with IP3K2^wy-74i)

(Dean et al., 2015)

Phenotype Manifest In

wing

(Dean et al., 2015, Lindsley and Zimm, 1992)

wing (with Df(1)Exel6245)

(Dean et al., 2015)

wing (with IP3K2^wy-1)

(Dean et al., 2015)

wing (with IP3K2^wy-74i)

(Dean et al., 2015)

Detailed Description

Statement

Reference

Particular wing phenotypes are seen in flies (percentages rounded) of the listed genotypes, (1) being the least severe - (1) a wave-like buckle along the costal vein (distal to its intersection with the subcostal region), (2) an upturn at the most distal margin of the wing, (3) overall shriveled morphology (distinguishable from mechanical damage or failure to inflate after eclosion); overall, IP3K2^wy-74i has the most severe phenotype, followed by IP3K2^wy-2 and IP3K2^wy-1 :

(1) IP3K2^wy-2/IP3K2^wy-2 3%, IP3K2^wy-1/IP3K2^wy-2 33%, IP3K2^wy-2/IP3K2^wy-74i 0%, IP3K2^wy-2/Df(1)Exel6245 0%;

(2) IP3K2^wy-2/IP3K2^wy-2 58%, IP3K2^wy-1/IP3K2^wy-2 53%, IP3K2^wy-2/IP3K2^wy-74i 36%, IP3K2^wy-2/Df(1)Exel6245 96%;

(3) IP3K2^wy-2/IP3K2^wy-2 39%, IP3K2^wy-1/IP3K2^wy-2 13%, IP3K2^wy-2/IP3K2^wy-74i 64%, IP3K2^wy-2/Df(1)Exel6245 4%.

(Dean et al., 2015)

More extreme than wy¹, more upward curl to wings.

(Lindsley and Zimm, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

IP3K2^wy-2 has visible phenotype, suppressible by Itpr^90B.0/Itp-r83A[+]

(Dean et al., 2015)

IP3K2^wy-2 has visible phenotype, suppressible by Itpr^ug3/Itp-r83A[+]

(Dean et al., 2015)

NOT suppressed by

Statement

Reference

IP3K2^wy-2 has visible phenotype, non-suppressible by IP3K1[+]/IP3K1^KG02192

(Dean et al., 2015)

IP3K2^wy-2 has visible phenotype, non-suppressible by Df(2L)ED49/+

(Dean et al., 2015)

IP3K2^wy-2 has visible phenotype, non-suppressible by Cam[+]/Camⁿ³³⁹

(Dean et al., 2015)

IP3K2^wy-2 has visible phenotype, non-suppressible by Cam⁷/Cam[+]

(Dean et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

IP3K2^wy-2 has wing phenotype, suppressible by Itpr^ug3/Itp-r83A[+]

(Dean et al., 2015)

IP3K2^wy-2 has wing phenotype, suppressible by Itpr^90B.0/Itp-r83A[+]

(Dean et al., 2015)

NOT suppressed by

Statement

Reference

IP3K2^wy-2 has wing phenotype, non-suppressible by IP3K1[+]/IP3K1^KG02192

(Dean et al., 2015)

IP3K2^wy-2 has wing phenotype, non-suppressible by Df(2L)ED49/+

(Dean et al., 2015)

IP3K2^wy-2 has wing phenotype, non-suppressible by Cam[+]/Camⁿ³³⁹

(Dean et al., 2015)

IP3K2^wy-2 has wing phenotype, non-suppressible by Cam⁷/Cam[+]

(Dean et al., 2015)

IP3K2^wy-2 has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Additional Comments

Genetic Interactions

Statement

Reference

IP3K1^KG02192/+, Df(2L)ED49/+, Camⁿ³³⁹/+ or Cam⁷/+ do not modify wing phenotypes in IP3K2^wy-2/IP3K2^wy-2 flies.

Itp-r83A^ug3/+ or Itp-r83A^90B.0/+ partially suppresses the wing phenotypes seen in sibling IP3K2^wy-2/IP3K2^wy-2 control flies.

(Dean et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

IP3K2^wy-2 is rescued by IP3K2^UAS.cDa

(Dean et al., 2015)

Comments

IP3K2^Scer\UAS.cDa (without a GAL4) fully rescues wing phenotypes in IP3K2^wy-2/IP3K2^wy-2 flies.

(Dean et al., 2015)

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (2)

Bloomington

84699

w^* IP3K2^wy-2 g² f^*

4355

y¹ N^fa-1 IP3K2^wy-2 g²

Notes on Origin

Discoverer

Ruch.

(Lindsley and Zimm, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

IP3K2^wy-2

cx-b

(Lindsley and Zimm, 1992)

wy²

(Dean et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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