FlyBase Allele Report: Dmel\Itpr[90B.0]

General Information

Symbol

Dmel\Itpr^90B.0

Species

D. melanogaster

Name

FlyBase ID

FBal0063165

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

Also Known As

l(3)itpr^90B.0, itpr^90B0, Itp-r83A^90B.0

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Venkatesh and Hasan, 1997)

Mutagen

P-element activity

(Venkatesh and Hasan, 1997)

Progenitor genotype

Cytology

Description

Deletion of at least 10kb, removing the Itp-r83A gene.

(Venkatesh and Hasan, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Twyning et al., 2024)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Twyning et al., 2024)

ameliorates amyotrophic lateral sclerosis

modeled by Hsap\TARDBP^UAS.cHa

(Kim et al., 2012)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cHa

(Balasubramanian and Srinivasan, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye photoreceptor cell | somatic clone

(Raghu et al., 2000)

interommatidial bristle | somatic clone

(Raghu et al., 2000)

rhabdomere | somatic clone

(Raghu et al., 2000)

Detailed Description

Statement

Reference

Itp-r83A^90B.0/+ flies have normal wing structure.

(Dean et al., 2015)

Itp-r83A^90B.0/+ 30-day-old adult males display learning defects as they fail to demonstrate significant decrease in courtship activity when paired with an mated unreceptive female but their short-term (60 min) memory is not impaired.

(McBride et al., 2010)

Whole cell patch-clamp recordings of dissociated ommatidia from Itp-r83A^90B.0 mutants shows that, after light stimulation, the Shab-mediated light-dependent modulation of the delayed rectifier current is indistinguishable from wildtype controls.

(Krause et al., 2008)

Heterozygous flies show an increased resistance to 1% H₂O₂ compared to control flies.

(Monnier et al., 2002)

Lethality acts in the second larval instar. First to second instar molting is delayed.

(Venkatesh et al., 2001)

Itp-r83A^90B.0 heterozygotes recover from butanol-induced adaptation faster than wild-type. The response of homozygous Itp-r83A^90B.0/+ animals to all concentrations of ethyl acetate is normal.

(Deshpande et al., 2000)

Homozygous clones in the retina can result in defects in the mechanosensory bristles of the ommatidia, including duplication and missing and misplaced bristles. Ultrastructurally, the morphology of the photoreceptors in homozygous clones appears completely normal in newly eclosed flies. However, the photoreceptors undergo light-independent degeneration; at 2 weeks after eclosion the first signs of degeneration are seen with a subtle deformity at the base of the rhabdomere membrane, while at 8 weeks after eclosion the photoreceptor cells become filled with sacs of vesicles and finally degenerate.

In whole-cell recordings of photoreceptors from flies in which the entire eye is homozygous for Itp-r83A^90B.0, quantum bumps are elicited with a quantum efficiency similar to that of wild type and with a similar latency and amplitude compared to wild type. The time courses of wild-type and mutant quantum bumps are indistinguishable. Light adaptation is unaffected in flies in which the entire eye is homozygous for Itp-r83A^90B.0.

(Raghu et al., 2000)

Homozygous embryos develop normally and hatch as viable first instar larvae. Lethality of homozygotes begins early in the second instar stage, 72 hours after egg-laying (AEL), and by 112 hours AEL nearly all homozygotes are dead. Homozygotes undergo the first larval moult more slowly than wild-type larvae.

(Venkatesh and Hasan, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has abnormal developmental rate phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-178

(Venkatesh et al., 2001)

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has lethal | larval stage phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-178

(Venkatesh et al., 2001)

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has lethal | pupal stage phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-178

(Venkatesh et al., 2001)

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has abnormal developmental rate phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-2080

(Venkatesh et al., 2001)

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has lethal | larval stage phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-2080

(Venkatesh et al., 2001)

Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has lethal | pupal stage phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-2080

(Venkatesh et al., 2001)

Itpr^90B.0 has abnormal developmental rate | recessive phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-2080

(Venkatesh et al., 2001)

Itpr^90B.0 has lethal | larval stage | recessive phenotype, enhanceable by Adcy1^rut-2080/Adcy1^rut-2080

(Venkatesh et al., 2001)

NOT suppressed by

Statement

Reference

Itpr^90B.0 has abnormal learning | male | adult stage phenotype, non-suppressible by Psn^B3/Psn[+]

(McBride et al., 2010)

Itpr^90B.0 has abnormal learning | male | adult stage phenotype, non-suppressible by Psn^I2/Psn[+]

(McBride et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^Toll-6-D42, NUCB1^NIG.32190R/Itpr^90B.0 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^Toll-6-D42

(Balasubramanian and Srinivasan, 2020)

NUCB1^c01508, Itpr^90B.0, NUCB1[+] is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\TARDBP^UAS.cHa, Scer\GAL4^Toll-6-D42

(Balasubramanian and Srinivasan, 2020)

Itpr^90B.0/Itp-r83A[+] is a suppressor of visible phenotype of IP3K2^wy-74i

(Dean et al., 2015)

Itpr^90B.0/Itp-r83A[+] is a suppressor of visible phenotype of IP3K2^wy-1

(Dean et al., 2015)

Itpr^90B.0/Itp-r83A[+] is a suppressor of visible phenotype of IP3K2^wy-2

(Dean et al., 2015)

Itpr^90B.0/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^90B.0/Itp-r83A[+] is a suppressor of abnormal memory | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

NOT Suppressor of

Statement

Reference

Itpr^90B.0/Itp-r83A[+] is a non-suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^B3

(McBride et al., 2010)

Itpr^90B.0/Itp-r83A[+] is a non-suppressor of abnormal learning | male | adult stage | progressive phenotype of Psn^I2

(McBride et al., 2010)

Other

Statement

Reference

Itpr^90B.0/Itpr^1664GAL4, Pka-R1^BDK.UAS, Scer\GAL4^{Itpr-1664GAL4} has lethal phenotype

(Venkatesh et al., 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

Itpr^90B.0/Itp-r83A[+] is a suppressor of wing phenotype of IP3K2^wy-74i

(Dean et al., 2015)

Itpr^90B.0/Itp-r83A[+] is a suppressor of wing phenotype of IP3K2^wy-1

(Dean et al., 2015)

Itpr^90B.0/Itp-r83A[+] is a suppressor of wing phenotype of IP3K2^wy-2

(Dean et al., 2015)

Other

Statement

Reference

Adcy1^rut-2080/Adcy1^rut-178, Itpr^90B.0 has larval tracheal system phenotype

(Venkatesh et al., 2001)

Adcy1^rut-2080, Itpr^90B.0 has larval tracheal system phenotype

(Venkatesh et al., 2001)

Adcy1^rut-2080, Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has larval tracheal system phenotype

(Venkatesh et al., 2001)

Adcy1^rut-2080/Adcy1^rut-178, Itpr^90B.0/Itpr⁰⁵⁶¹⁶ has larval tracheal system phenotype

(Venkatesh et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Itp-r83A^90B.0/+ partially suppresses wing phenotypes seen in IP3K2^wy-1/IP3K2^wy-1, IP3K2^wy-2/IP3K2^wy-2 or IP3K2^wy-74i/IP3K2^wy-74i flies.

(Dean et al., 2015)

The learning defects (measured in a courtship behavior assay) observed in the Psn^B3, Psn^I2 or the Itp-r83A^90B.0 single heterozygote 30 day-old males are not suppressed in either the Psn^B3/+;Itp-r83A^90B.0/+ or the Psn^I2/+;Itp-r83A^90B.0/+ double heterozygotes. Combination with a single copy of Itp-r83A^90B.0 can however restore the short-term memory defects characteristic for both Psn^B3 and Psn^I2 aged heterozygote males (both at 30 and 40 days of age).

(McBride et al., 2010)

Double mutants with rut alleles show inability to develop beyond the first larval instar, though some of the larvae show spiracles and mouthparts characteristic of second instars, without having molted. The developmental delay of Itp-r83A^90B.0/Itp-r83A^1664GAL4 transheterozygotes is not affected by P{UAS-PKA.mC} expression.

(Venkatesh et al., 2001)

Xenogenetic Interactions

Statement

Reference

Itp-r83A^90B.0 partially suppresses the climbing defect of 15-day old Scer\GAL4^D42>Hsap\TARDBP^Scer\UAS.cHa flies.

(Kim et al., 2012)

Complementation and Rescue Data

Complements

Itpr^hc1

(Joshi et al., 2004)

Itpr^hc7

(Joshi et al., 2004)