FlyBase Allele Report: Dmel\Itpr[UAS.cVa]

General Information

Symbol

Dmel\Itpr^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Venkatesh

FlyBase ID

FBal0124164

Feature type

allele

Associated gene

Dmel\Itpr

Associated Insertion(s)

Carried in Construct

P{UAS-Itpr.V}

Also Known As

UAS-itpr, UASitpr⁺, UAS-IP3R, UAS-InsP3R

Key Links

Transgenic product class

cDNA

(Venkatesh et al., 2001)

wild_type

(Venkatesh et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Venkatesh et al., 2001)

wild_type

(Venkatesh et al., 2001)

Mutagen

in vitro construct

(Venkatesh et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-Itpr.V}

Cytology

Description

Expression of Itp-r83A embryonic splice form cDNA is governed by UASt regulatory sequences.

(Venkatesh et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Itpr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 16

modeled by Hsap\SIGMAR1^E102Q.UAS

(Couly et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size, with Itpr^sv35/Itpr^ug3, Scer\GAL4^Ddc.PL

(Agrawal et al., 2009)

abnormal body size, with Itpr^sv35/Itpr^ug3, Scer\GAL4^Ilp2.PR

(Agrawal et al., 2009)

decreased cell number | larval stage, with Scer\GAL4^Tep4.PU

(Destalminil-Letourneau et al., 2021)

viable, with Itpr^ka901/Itpr^ug3, Scer\GAL4^arm.PS

(Joshi et al., 2004)

Phenotype Manifest In

embryonic/larval crystal cell | decreased number | larval stage, with Scer\GAL4^Tep4.PU

(Destalminil-Letourneau et al., 2021)

Detailed Description

Statement

Reference

Expression of Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^{Act5C.Switch.PR} (in females fed RU486 during adulthood) does not significantly affect lifespan compared to controls.

(Parkhitko et al., 2016)

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR does not result in bigger sized pupae compared to wild-type.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 flies results in significantly larger pupal size compared to wild-type pupae, and adult flies are also increased in size. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL in Itp-r83A^sv35/Itp-r83A^ug3 flies results in increased pupal length, body size, and adult fly weights. There is a significant increase in the time taken to pupate by mutant larvae compared to wild-type.

Itp-r83A^sv35/Itp-r83A^ug3 animals expressing Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^12.1 and either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL have more surviving pupae, and pupate 12-20 hours earlier than when expression is driven by Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL alone. Pupal and adult fly sizes in mutant animals expressing Itp-r83A^sv35/Itp-r83A^ug3 animals expressing Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^12.1 and either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL are comparable to that of wild-type animals.

(Agrawal et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Itpr^UAS.cVa, Scer\GAL4^Tep4.PU has decreased cell number | larval stage phenotype, enhanceable by btl^RNAi.UAS.cUa, Scer\GAL4^Tep4.PU

(Destalminil-Letourneau et al., 2021)

Suppressor of

Statement

Reference

Itpr^UAS.cVa, Scer\GAL4^{Itpr-1664GAL4} is a suppressor of abnormal developmental rate phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^{Itpr-1664GAL4}

(Venkatesh et al., 2001)

NOT Suppressor of

Statement

Reference

Itpr^UAS.cVa, Scer\GAL4^MESO.dome is a non-suppressor of abnormal mitotic cell cycle | larval stage phenotype of Nos^RNAi.UAS.cCa, Scer\GAL4^MESO.dome

(Cho et al., 2024)

Itpr^UAS.cVa, Scer\GAL4^{Itpr-1664GAL4} is a non-suppressor of abnormal developmental rate phenotype of Pka-R1^BDK.UAS, Scer\GAL4^{Itpr-1664GAL4}

(Venkatesh et al., 2001)

Other

Statement

Reference

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has visible | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has increased cell death | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased cell number | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has decreased rate of adult locomotory behavior phenotype

(Ham et al., 2023)

Phenotype Manifest In

Enhanced by

Statement

Reference

Itpr^UAS.cVa, Scer\GAL4^Tep4.PU has embryonic/larval crystal cell | larval stage | decreased number phenotype, enhanceable by btl^RNAi.UAS.cUa, Scer\GAL4^Tep4.PU

(Destalminil-Letourneau et al., 2021)

NOT Suppressor of

Statement

Reference

Itpr^UAS.cVa, Scer\GAL4^MESO.dome is a non-suppressor of embryonic/larval lymph gland phenotype of Nos^RNAi.UAS.cCa, Scer\GAL4^MESO.dome

(Cho et al., 2024)

Other

Statement

Reference

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has wing phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult thorax phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult thorax phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has wing phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult thorax phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has wing phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult thorax phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^KK109403, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has wing phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult thorax phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has wing phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult thorax phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has flight muscle cell phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^KO, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has mitochondrion | adult stage phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Pink1^B9, Scer\GAL4^hs.PB has adult dopaminergic neuron | decreased number phenotype

(Ham et al., 2023)

Cisd^GD15791, Itpr^UAS.cVa, Scer\GAL4^hs.PB, park¹ has wing phenotype

(Ham et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

The obesity (measured as fat/protein ratio) of THADA^KO1 mutant adults is partially rescued by Scer\GAL4^tub.PU-controlled expression of Itp-r83A^Scer\UAS.cVa.

(Moraru et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Ilp2.PR, Scer\GAL80^ts.αTub84B, Itpr^UAS.cVa rescues Itpr^ug3/Itpr^ka1091

(Agrawal et al., 2010)

Scer\GAL4^elav-C155/Itpr^UAS.cVa rescues Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2006)

Scer\GAL4^hs.PB/Itpr^UAS.cVa rescues Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2004)

Scer\GAL4^Ddc.PL/Itpr^UAS.cVa rescues Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2004)

Scer\GAL4^hs.PB/Itpr^UAS.cVa rescues Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^Ddc.PL/Itpr^UAS.cVa rescues Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^arm.PS/Itpr^UAS.cVa rescues Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Partially rescues

Scer\GAL4^Ilp2.PR/Itpr^UAS.cVa partially rescues Itpr^ug3/Itpr^ka1091

(Subramanian et al., 2013)

Scer\GAL4^elav-C155/Itpr^UAS.cVa partially rescues Itpr^ug3/Itpr^ka1091

(Agrawal et al., 2010)

Scer\GAL4^Ilp2.PR/Itpr^UAS.cVa partially rescues Itpr^ug3/Itpr^ka1091

(Agrawal et al., 2010)

Scer\GAL4^Ilp2.PR/Itpr^UAS.cVa partially rescues Itpr^wc703/Itpr^ug3

(Agrawal et al., 2010)

Scer\GAL4^Ilp2.PR/Itpr^UAS.cVa partially rescues Itpr^sv35/Itpr^ug3

(Agrawal et al., 2009)

Scer\GAL4^Ddc.PL/Itpr^UAS.cVa partially rescues Itpr^sv35/Itpr^ug3

(Agrawal et al., 2009)

Scer\GAL4^Ilp2.PR, Scer\GAL4^Ddc.PL, Itpr^UAS.cVa partially rescues Itpr^sv35/Itpr^ug3

(Agrawal et al., 2009)

Scer\GAL4^hs.PB/Itpr^UAS.cVa partially rescues Itpr^sa54/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^{neur-GAL4-A101}/Itpr^UAS.cVa partially rescues Itpr^sa54/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^{neur-GAL4-A101}/Itpr^UAS.cVa partially rescues Itpr^ug3/Itpr^ka1091

(Banerjee et al., 2004)

Scer\GAL4^Ddc.PL/Itpr^UAS.cVa partially rescues Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Scer\GAL4^dimm-929/Itpr^UAS.cVa partially rescues Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Scer\GAL4^elav-C155/Itpr^UAS.cVa partially rescues Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Scer\GAL4^pros.PMG/Itpr^UAS.cVa partially rescues Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Scer\GAL4^{neur-GAL4-A101}/Itpr^UAS.cVa partially rescues Itpr^ug3/Itpr^ka1091

(Joshi et al., 2004)

Scer\GAL4^hs.PU/Itpr^UAS.cVa partially rescues Itpr^90B.0/Itpr⁰⁵⁶¹⁶

(Venkatesh et al., 2001)

Fails to rescue

Scer\GAL4^elav.PLu/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^ChAT.7.4/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^RapGAP1-OK6/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^{shakB.lethal.4.1}/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^Mef2.PR/Itpr^UAS.cVa fails to rescue Itpr^wc703/Itpr^ug3

(Banerjee et al., 2004)

Scer\GAL4^12.1/Itpr^UAS.cVa fails to rescue Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Scer\GAL4^ChAT.7.4/Itpr^UAS.cVa fails to rescue Itpr^ka901/Itpr^ug3

(Joshi et al., 2004)

Comments

Expression of Itp-r83A^Scer\UAS.cVa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR rescues the starvation resistance seen in itgr[ku] (Itp-r83A^ka1091/Itp-r83A^ug3) mutant flies to wild type levels. The lipid level defects in the fat body cells of starved flies is also rescued, as is the increase in body weight and TAG level. Expression in the fat body under the control of Scer\GAL4^c729 partially rescues the starvation resistance phenotype, increase in body weight and abdominal TAG level.

Expression of Itp-r83A^Scer\UAS.cVa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR partially restores the profile of TAGs and their constituent fatty acids in Itp-r83A^ka1091/Itp-r83A^ug3 mutants close to that of the wild type under starvation.

Expression of Itp-r83A^Scer\UAS.cVa in the insulin-producing cells under the control of Scer\GAL4^Ilp2.PR suppresses the overeating seen in itgr[ku] (Itp-r83A^ka1091/Itp-r83A^ug3) mutant females.

(Subramanian et al., 2013)

Complete rescue of the altered wing posture is seen in Itp-r83A^Scer\UAS.cVa; Scer\GAL4^Ilp2.PR/+; Itp-r83A^ka1091/Itp-r83A^ug3 animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A^ka1091/Itp-r83A^ug3 flies expressing Scer\GAL4^Ilp2.PR-driven Itp-r83A^Scer\UAS.cVa leads to rhythmic generation of action potentials similar to what is observed in wild-type flies. However, the response in some flies is more erratic and could not be maintained for more than five seconds.

Complete rescue of the altered wing posture is seen in Itp-r83A^Scer\UAS.cVa; Scer\GAL4^Ilp2.PR/+; Itp-r83A^wc703/Itp-r83A^ug3 animals. Flight ability is also rescued significantly in these flies. The delivery of an air puff stimulus to Itp-r83A^wc703/Itp-r83A^ug3 flies expressing Scer\GAL4^Ilp2.PR-driven Itp-r83A^Scer\UAS.cVa leads to rhythmic generation of action potentials, but in most flies the air puff response lasts for a shorter period of time compared with the control flies.

Wing posture and air puff-induced flight (assessed in single fly tethered assays) are not rescued when Itp-r83A^Scer\UAS.cVa; Scer\GAL4^Ilp2.PR/Scer\GAL80[ts.αTub84B]; Itp-r83A^ka1091/Itp-r83A^ug3 animals are grown at 19[o]C during pupal and adult stages, but are completely rescued when these animals are grown at 30[o]C. Itp-r83A^Scer\UAS.cVa; Scer\GAL4^Ilp2.PR/Scer\GAL80[ts.αTub84B]; Itp-r83A^ka1091/Itp-r83A^ug3 organisms grown at 30[o]C during pupal stages, but shifted to 19[o]C as adults, have normal wing posture and air puff-induced flight.

Expression of Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^elav-C155 can partially rescue the wing posture and physiology defects of Itp-r83A^ka1091/Itp-r83A^ug3 mutants, but it is insufficient for restoring flight ability.

Pan-neuronal expression of Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^elav-C155 in a Itp-r83A^ka1091/Itp-r83A^ug3 background restores both store operated Ca2[2+] entry and Ca[2+] to levels seen in wild-type. .

Pan-neuronal expression of Itp-r83A^Scer\UAS.cVa driven by either Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL in a Itp-r83A^ka1091/Itp-r83A^ug3 background restores both store operated Ca2[2+] entry and Ca[2+] to levels seen in wild-type. .

(Agrawal et al., 2010)

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae significantly rescues reduced larval body size. Approximately half the surviving larvae can pupate and emerge as adults.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae significantly rescues reduced larval body size and lethality.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL and Scer\GAL4^Ilp2.PR in Itp-r83A^sv35/Itp-r83A^ug3 mutant larvae rescues body size and lethality. Survival is not improved beyond that observed with over-expression under the control of only Scer\GAL4^Ddc.PL alone.

Defective feeding behaviour in Itp-r83A^sv35/Itp-r83A^ug3 larvae can be rescued by expression of Itp-r83A^Scer\UAS.cVa under the ontrol of Scer\GAL4^Ilp2.PR or Scer\GAL4^Ddc.PL.

(Agrawal et al., 2009)

Itp-r83A^Scer\UAS.cVa; Scer\GAL4^elav-C155 rescues lethality in Itp-r83A^ka1091/Itp-r83A^ug3 animals raised at 17'C.

(Banerjee et al., 2006)

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^hs.PB rescues flightlessness in 30% of Itp-r83A^sa54/Itp-r83A^ug3 flies; the remaining flies still show crumples wing margins.

Expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^{neur-GAL4-A101} rescues the wing posture defects of Itp-r83A transheterozygotes but not the flightlessness nor the flight physiology phenotypes. In contrast, expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^Ddc.PL rescues the wing position, flight and flight physiology of these mutants.

Rescue of Itp-r83A^wc703/Itp-r83A^ug3 flies by expression of Itp-r83A^Scer\UAS.cVa under the control of Scer\GAL4^hs.PB following a heat shock shows that expression during early pupal development is sufficient to rescue flight behavior and physiology, while expression after adult emergence is ineffective in rescuing flight.

(Banerjee et al., 2004)

Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^arm.PS nearly rescues the cold sensitive lethality of Itp-r83A^ug3/Itp-r83A^ka901 animals. The delay in the second to third instar and third instar to pupae transition is rescued only partly. Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^elav-C155 completely rescues the second instar lethality and restores a normal transition from second to third instar. These animals are unable to eclose as adults. Itp-r83A^Scer\UAS.cVa driven by Scer\GAL4^Ddc.PL or Scer\GAL4^dimm-929 are able to partially rescue the second to third instar lethality.

(Joshi et al., 2004)

Full rescue is observed as judged by rescue of developmental delay and lethality up to 136hr after egg laying, but only partial rescue of adult lethality is evident.

(Venkatesh et al., 2001)

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Mutant

Wild-type

Stocks (1)

Bloomington

30742

P{UAS-Itpr.V}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

InsP3R^OE

(He et al., 2018)

Itp-r83A^Scer\UAS.cVa

Itp-r83A^UAS.cVa

Itpr^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Venkatesh

(Venkatesh et al., 2001)

Secondary FlyBase IDs

References (22)

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