FlyBase Allele Report: Dmel\Cyt-c-d[bln1]

General Information

Symbol

Dmel\Cyt-c-d^bln1

Species

D. melanogaster

Name

FlyBase ID

FBal0027976

Feature type

allele

Associated gene

Dmel\Cyt-c-d

Associated Insertion(s)

P{PZ}Cyt-c-d^bln1

Carried in Construct

Also Known As

bln¹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Arama et al., 2006, Arama et al., 2003)

Mutagen

P-element activity

(Castrillon et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}Cyt-c-d^bln1

Cytology

Description

The P{PZ}Cyt-c-d^bln1 insertion maps within both the Cyt-c-d and CG31808 transcription units (overlapping genes). In Cyt-c-d, the insertion site is close to the boundary of the first exon and intron. FlyBase curator comment: FBrf0190354 demonstrates that the mutant phenotype of the P{PZ}Cyt-c-d^bln1 insertion is caused by an effect on Cyt-c-d.

(Huh et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,716,217..16,716,217 [+]

(Arama et al., 2006, Huh et al., 2004, Arama et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Arama et al., 2006, Arama et al., 2003)

male sterile (with Df(2L)H20)

(Arama et al., 2003)

male sterile | recessive

(Spradling et al., 1999)

viable

(Arama et al., 2003)

Phenotype Manifest In

major mitochondrial derivative

(Huh et al., 2004)

minor mitochondrial derivative

(Huh et al., 2004)

secondary pigment cell

(Mendes et al., 2006)

spermatid

(Arama et al., 2006, Huh et al., 2004)

spermatid axoneme

(Huh et al., 2004)

tertiary pigment cell

(Mendes et al., 2006)

Detailed Description

Statement

Reference

Cyt-c-d^bln1 mutants show defects in spermatid individualization.

(Arama et al., 2006)

At 42 hours after puparium formation (APF), homozygous retinas have extra interommatidial cells compared to wild type.

(Mendes et al., 2006)

Lysates from 4 hour old prepupae heterozygous or homozygous for Cyt-c-d^bln1 show no reduction in caspase activation compared to wild type.

(Dorstyn et al., 2004)

Cysts from mutant male testes show multiple defects in spermatogenesis prior to individualisation, including failure to carry out polyglycylation of axonemal microtubules, and aberrant development of the major and minor mitochondrial derivatives.

(Huh et al., 2004)

Cyt-c-d^bln1/Df(2L)H20 are infertile. Spermatogenesis is normal up to the individuation stage and spermatids form mature looking nuclei. Although the initial assembly of the "individualisation complex" at the head of the cyst appears normal, there is a failure of cytoplasm to collect into a normal cystic bulge and to translocate caudally. In addition, these cysts lack the caspase activation seen at this stage in wild-type cysts.

(Arama et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cyt-c-d^bln1 has male sterile phenotype, suppressible by Cyt-c-p^{Cyt-c-d.UTR.Hsp83}

(Arama et al., 2006)

Suppressor of

Statement

Reference

Cyt-c-d^bln1/Cyt-c-d[+] is a suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

BacA\p35^GMR.PH, Cyt-c-d^bln1, Cyt-c-d[+] is a suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Cyt-c-d^bln1/Cyt-c-d[+] is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cyt-c-d^bln1 has spermatid phenotype, suppressible by Cyt-c-p^{Cyt-c-d.UTR.Hsp83}

(Arama et al., 2006)

Suppressor of

Statement

Reference

Cyt-c-d^bln1/Cyt-c-d[+] is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

BacA\p35^GMR.PH, Cyt-c-d^bln1, Cyt-c-d[+] is a suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Cyt-c-d^bln1/Cyt-c-d[+] is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Cyt-c-d^bln1/Cyt-c-d[+] is a suppressor of eye phenotype of Dref^UAS.cSa

(Ida et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of the Cyt-c-p^{Cyt-c-d.UTR.Hsp83} transgene in Cyt-c-d^bln1 mutants rescues the spermatid individualization and male sterility phenotypes.

(Arama et al., 2006)

Xenogenetic Interactions

Statement

Reference

A Cyt-c-d^bln1/+ background rescues the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

A BacA\p35^GMR.PH/+ background strongly rescues the adult eye size defect found upon expression of Mmus\Gria1^Lc.Scer\UAS under the control of Scer\GAL4^hs.2sev in a Cyt-c-d^bln1/+ background.

(Yang et al., 2013)

Complementation and Rescue Data

Fails to complement

Cyt-c-d^Z21091

(Arama et al., 2006)

Rescued by

Cyt-c-d^bln1 is rescued by Cyt-c-d^Hsp83.PA

(Arama et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11763

P{PZ}Cyt-c-d^bln1 CG31808^bln-1 cn¹/CyO; ry⁵⁰⁶

Kyoto

101994

P{PZ}Cyt-c-d^bln1 cn¹ / CyO ; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Male sterility can be reverted by precise excision of Cyt-c-d^bln1.

(Arama et al., 2003)

Cyt-c-d^bln1 does not affect transcription of Cyt-c-p.

(Arama et al., 2006)

Complements: dac^P.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Cyt-c-d⁰⁴⁴⁴⁵

Cyt-c-d^bln-1

Cyt-c-d^bln1

(Ida et al., 2009)

bln¹

(Rathke et al., 2007, Dorstyn et al., 2004, Huh et al., 2004, Arama et al., 2003, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

cyt c-d^bln1

(Mendes et al., 2006)

cyt-c-d^bln1

(Yang et al., 2013)

ms(2)04445

(Spradling et al., 1999)

ms(2)04445⁰⁴⁴⁴⁵

Name Synonyms

blanks

(Dorstyn et al., 2004)

Secondary FlyBase IDs

FBal0156749

References (13)

Report Sections

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