FlyBase Allele Report: Dmel\ds[33k]

General Information

Symbol

Dmel\ds^33k

Species

D. melanogaster

Name

FlyBase ID

FBal0028155

Feature type

allele

Associated gene

Dmel\ds

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Clark et al., 1995)

Mutagen

spontaneous

(Clark et al., 1995)

X ray

Progenitor genotype

Caused by aberration

In(2LR)bw^V1

Cytology

Description

Break at 21D falls within the large intron of ds. Predicted product will be a secreted and truncated protein.

(Clark et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity (with ds^UAO71)

(Strutt and Strutt, 2002)

visible (with ds^38k)

(Rodriguez, 2004)

visible | recessive

(Adler et al., 1998)

Phenotype Manifest In

anterior crossvein

(Clark et al., 1995)

axon & larval optic lobe

(Dearborn and Kunes, 2004)

costal vein | ectopic (with ds^38k)

(Rodriguez, 2004)

embryonic/larval tracheal dorsal trunk (with Df(2L)Exel8003)

(Chung et al., 2009)

eye

(Clark et al., 1995)

glial cell & larval optic lobe

(Dearborn and Kunes, 2004)

humeral sclerite | ectopic (with ds^38k)

(Rodriguez, 2004)

leg

(Clark et al., 1995)

scutellum | ectopic (with ds^38k)

(Rodriguez, 2004)

scutum | ectopic (with ds^38k)

(Rodriguez, 2004)

tegula | ectopic (with ds^38k)

(Rodriguez, 2004)

thorax

(Clark et al., 1995)

wing

(Adler et al., 1998, Clark et al., 1995)

wing (with ds^38k)

(Rodriguez, 2004)

wing | posterior (with ds^38k)

(Rodriguez, 2004)

wing | proximal

(Adler et al., 1998)

wing hair

(Adler et al., 1998)

wing hair (with ds^UAO71)

(Strutt and Strutt, 2002)

wing hinge (with ds^38k)

(Rodriguez, 2004)

wing hinge | posterior (with ds^38k)

(Rodriguez, 2004)

wing margin | anterior | ectopic (with ds^38k)

(Rodriguez, 2004)

Detailed Description

Statement

Reference

ds^33k/Df(2L)Exel8003 stage 16 embryos have longer dorsal trunks than normal.

(Chung et al., 2009)

Scaffold axons project aberrantly in the larval optic lobes if ds^33k mutants. Glia line these aberrant pathways and accumulate in abnormal destinations such as the lobula cortex.

(Dearborn and Kunes, 2004)

Occasionally in ds^38k/ds^33k escapers (3 out of 72 heminota scored), the wing is replaced by a winglet and, posterior to it, a lateral protuberance that looks like an scutum and scutellum. The winglet is composed of proximal anterior structures arranged in a mirror-image duplication. The smallest examples of these winglets consist of a mirror image duplication of anterior hinge structures - the tegula and humeral sclerite. Larger examples also have a rudimentary wing blade consisting of a mirror image duplication of anterior structures - the costa and anterior wing margin.

(Rodriguez, 2004)

The wings of ds^33k/ds^UAO71 animals have a trichome swirling pattern that is distinct from that due to fz¹⁵/fz²⁵.

(Strutt and Strutt, 2002)

ds^33k/ds^UAO71 flies have reduced viability, with only approximately half the expected number of adults emerging. Most of the adults that emerge quickly get stuck in the food and die. These flies also have a severely distorted body shape. ds^33k/ds^UAO71 flies have a wing tissue polarity phenotype similar to that of ds^UAO71 homozygotes and hemizygotes. There is a region at the anterior margin with a swirl of partially reversed polarity hairs. In the anterior/central proximal part of the wing blade there are regions of completely reversed polarity (hairs pointing proximally). These regions are separated from regions of relatively normal polarity by a region of swirling. The polarity disruptions are more severe on the ventral surface of the wing than the dorsal surface. Prehairs initiate in the vicinity of the distal vertex of the wing cells (as in wild-type) in wing regions of ds^33k/ds^UAO71 flies that do not show substantial polarity alterations. In most regions with abnormal polarity, prehairs initiate at an alternative location along the cell periphery. In the regions with reversed polarity, prehairs initiate at the proximal-most vertex, while in regions where hair polarity is close to random and in the regions at the centre of swirls, prehairs initiate in the central regions of the cells. Approximately half the wings of ds^33k/ds¹ flies have a very weak tissue polarity phenotype, consisting of a couple of multiple hair cells and/or a small region of polarity disruption in the proximal part of the wing.

(Adler et al., 1998)

In addition to 100% penetrant defects in leg, wing and thorax, rough patches in the eye appear at low frequency. Anterior crossvein is displaced posteriorly. Combinations of ds^33k and ds⁵⁵ with each other or with Df(2L)S2 or Df(2L)ast1 eclose with very low frequency and show very strong deformations of thorax, legs and wings. Imaginal discs of ds^M213/ds^M213 or ds^M213/ds^33k are within the size range of normal discs, showing no tendency to overgrowth.

(Clark et al., 1995)

ds^33k/+ has phenotype like ds^W/+; scutellar bristles wide apart; wings shorter and wider than normal; crossveins close together. ds^33k/ds^W has extreme phenotype like ds^d. RK3A. ds^33k has not been separated from the recessive lethal factor of In(2LR)bw^V1 and, therefore, has not been observed in homozygous condition.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ds^33k has visible | recessive phenotype, suppressible by fz^I.hs

(Adler et al., 1998)

NOT Enhancer of

Statement

Reference

ds^33k/ds[+] is a non-enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

ds^33k/ds[+] is a non-enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

NOT Suppressor of

Statement

Reference

ds^33k/ds[+] is a non-suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

ds^33k/ds[+] is a non-suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

ds^33k has wing phenotype, suppressible by fz^I.hs

(Adler et al., 1998)

ds^33k has wing hair phenotype, suppressible by fz^I.hs

(Adler et al., 1998)

Enhancer of

Statement

Reference

ds^33k/ds¹ is an enhancer of phenotype of fz^unspecified

(Adler et al., 1998)

ds^33k is an enhancer of phenotype of fz^unspecified

(Adler et al., 1998)

ds^33k/ds¹ is an enhancer of phenotype of fz²⁴

(Adler et al., 1998)

ds^33k is an enhancer of phenotype of fz²⁴

(Adler et al., 1998)

NOT Enhancer of

Statement

Reference

ds^33k/ds[+] is a non-enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

ds^33k/ds[+] is a non-enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

NOT Suppressor of

Statement

Reference

ds^33k/ds[+] is a non-suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

ds^33k/ds[+] is a non-suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The distinctive ds polarity phenotype of ds^33k/ds^UAO71 flies (regions of reversed polarity on the wing) is suppressed by expression of fz^I.hs 24 hours after white prepupa formation using a 1 hour heat shock. The appendage shape abnormalities and wing vein placement abnormalities are not suppressed. The fraction of the wing that shows abnormal polarity in fz²⁴ flies is increased if the fly also carries ds¹/ds^33k. The domineering nonautonomy of fz¹ clones is enhanced by ds^UAO71/ds^33k.