FlyBase Allele Report: Dmel\RpS6[WG1288]

General Information

Symbol

Dmel\RpS6^WG1288

Species

D. melanogaster

Name

FlyBase ID

FBal0030172

Feature type

allele

Associated gene

Dmel\RpS6

Associated Insertion(s)

P{lArB}RpS6^WG1288

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Watson et al., 1992)

Mutagen

Delta2-3 transposase

(Watson et al., 1992)

P-element activity

(Stewart and Denell, 1993)

Progenitor genotype

Associated Insertion(s)

P{lArB}RpS6^WG1288

Cytology

Description

P{lArB} insertion 12bp 5' from the origin of the RpS6 cDNA.

(Stewart and Denell, 1993)

Insertion WG1288 of Bellen, Genes Dev 3:1288 , within 10bp of site of insertion of P in RpS6^air8 in 5' untranslated region of RpS6. Impossible to determine precise insertion site as a small duplication accompanied insertion of P{lArB} element.

(Watson et al., 1992)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | dominant

(Lin et al., 2011)

lethal | recessive

(Stewart and Denell, 1993)

lethal - all die before end of larval stage | recessive

(Watson et al., 1992)

Minute

(Marygold et al., 2007)

some die during larval stage | recessive

(Watson et al., 1992)

Phenotype Manifest In

embryonic/larval lymph gland

(Stewart and Denell, 1993)

prothoracic gland

(Lin et al., 2011)

Detailed Description

Statement

Reference

RpS6^WG1288/RpS6^WG1288 embryonic macrophages do not show any changes in the phagocytic index (apoptotic cells clearance) compared to wild-type.

(Xiao et al., 2015)

The prothoracic gland is 35% smaller than normal in heterozygous third instar larvae.

Heterozygotes show a delay in eclosion of up to 18 hours compared to wild type. This delay is reduced by the addition of 20-hydroxyecdysone.

(Lin et al., 2011)

Hemizygous males are developmentally delayed at all stages. Lethality generally occurs during extended third instar period when melanotic tumours arise.

(Stewart and Denell, 1993)

Melanotic tumour phenotype similar to that of RpS6^air8.

(Watson et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RpS6^WG1288/RpS6[+] is a suppressor of visible phenotype of CycE^JP

(Lin et al., 2011)

NOT Suppressor of

Statement

Reference

RpS6^WG1288/RpS6[+] is a non-suppressor of decreased occurrence of cell division | larval stage phenotype of CycE^JP

(Lin et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

RpS6^WG1288/RpS6^WG1288 is a suppressor of embryonic/larval plasmatocyte | embryonic stage phenotype of pall^ko

(Xiao et al., 2015)

RpS6^WG1288/RpS6[+] is a suppressor of eye phenotype of CycE^JP

(Lin et al., 2011)

NOT Suppressor of

Statement

Reference

RpS6^WG1288/RpS6[+] is a non-suppressor of embryonic/larval plasmatocyte | embryonic stage phenotype of pall^ko

(Xiao et al., 2015)

RpS6^WG1288/RpS6[+] is a non-suppressor of eye disc phenotype of CycE^JP

(Lin et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The defects in phagocytic apoptotic cells clearance displayed by homozygous pall^ko mutant embryos can be rescued by combination with RpS6^WG1288 in homozygous, but not heterozygous state.

(Xiao et al., 2015)

RpS6^WG1288/+ restores eye size and reduces eye roughness in CycE^JP homozygous adults. The number of mitotic cells in the second mitotic wave in the CycE^JP eye disc is not increased by RpS6^WG1288/+.

The addition of 20-hydroxyecdysone prevents the suppression of the CycE^JP eye phenotype by RpS6^WG1288/+.

(Lin et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

RpS6^air8

(Stewart and Denell, 1993)

Rescued by

RpS6^WG1288 is rescued by Scer\GAL4^P0206/RpS6^UAS.cLa

(Lin et al., 2011)

Comments

Expression of RpS6^Scer\UAS.cLa under the control of Scer\GAL4^P0206 suppresses the developmental delay seen in RpS6^WG1288/+ flies.

(Lin et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Lethality is reverted by excision of the P{lArB} element, demonstrating the element to be responsible for the mutant phenotype.

(Stewart and Denell, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RpS6^WG1288

(Xiao et al., 2015, Lin et al., 2011)

hen²

(Stewart and Denell, 1993)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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