FlyBase Allele Report: Dmel\rho[PΔ38]

General Information

Symbol

Dmel\rho^PΔ38

Species

D. melanogaster

Name

FlyBase ID

FBal0033086

Feature type

allele

Associated gene

Dmel\rho

Associated Insertion(s)

Carried in Construct

Also Known As

rho^Δ38, rho³⁸, rho^P38

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Stemerdink and Jacobs, 1997)

loss of function allele

(Gabay et al., 1997, zur Lage et al., 1997, Freeman et al., 1992)

Mutagen

Delta2-3 transposase

(Freeman et al., 1992)

Progenitor genotype

P{lwB}X81

(Freeman et al., 1992)

Cytology

Description

Deletion removing whole of P{lwB}X81 and extending to between 88 nucleotides 3' of the presumed initiating ATG and 267 nucleotides 3' to this ATG.

(Freeman et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Freeman et al., 1992)

some die during embryonic stage | recessive

(Freeman et al., 1992)

Phenotype Manifest In

Detailed Description

Statement

Reference

rho^PΔ38 mutant clones generated in third instar larval eye discs occasionally show some morphological defects in the visual system, although lamina cartridge neuron differentiation appears unaffected. The 'on/off transients" (representing the activity of post-synaptic lamina cartridge neurons) that are normally seen in wild type are still present.

(Yogev et al., 2010)

The overall morphology of lch5 chordotonal organs is disrupted in rho^PΔ38 mutant embryos. In addition, these organs have only 1 cap attachement cell rather than the 2 per organ seen in wild-type, while lch5 ligament attachment cells are often absent or deformed.

(Inbal et al., 2004)

Homozygous embryos show loss of VA2 muscle precursor cells (only 2.3% of hemisegments contain VA2 precursor cells).

(Johnson Hamlet and Perkins, 2001)

The number of midline glia cells surviving to stage 16 is decreased to an average of 0.9 +/- 0.1 cells per segment in homozygous embryos.

(Stemerdink and Jacobs, 1997)

Mutant embryos exhibit tracheal phenotype.

(Wappner et al., 1997)

In stage 12/3 homozygous embryos the commissures are thicker and narrower than wild type and by stage 14 they remain incompletely separated. In stage 12/5 embryos the wild type number of midline glia are present but appear tightly clustered and displayed dorsally. Reduction in cell number from stage 13 until 14. Reduction in the VUM cell number at stage 11, during stage 13 half the wild type number of neurons migrate ventrally, these are still present at stage 14. All MP1 neurons are present at stage 13 but are disconnected from each other, by stage 14 this separation has grown. In stage 14 embryos there is a reduced number of en⁺ neurons present at the midline.

(Sonnenfeld and Jacob, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

rho^PΔ38 has muscle cell of ventral acute muscle 2 | precursor phenotype, suppressible by Scer\GAL4^twi.PG/csw^{UAS.Tag:Myr(Src64B)}

(Johnson Hamlet and Perkins, 2001)

NOT Enhancer of

Statement

Reference

rho^PΔ38 is a non-enhancer of cephalopharyngeal skeleton phenotype of Krn^UAS.cRa, Scer\GAL4^69B

(Reich and Shilo, 2002)

rho^PΔ38 is a non-enhancer of wing phenotype of Krn^UAS.cRa, Scer\GAL4^Bx-MS1096

(Reich and Shilo, 2002)

NOT Suppressor of

Statement

Reference

rho^PΔ38/rho^PΔ38 is a non-suppressor of lch5 ligament attachment cell | increased number phenotype of Scer\GAL4^repo, spi^s.UAS

(Inbal et al., 2004)

rho^PΔ38 is a non-suppressor of cephalopharyngeal skeleton phenotype of Krn^UAS.cRa, Scer\GAL4^69B

(Reich and Shilo, 2002)

rho^PΔ38 is a non-suppressor of wing phenotype of Krn^UAS.cRa, Scer\GAL4^Bx-MS1096

(Reich and Shilo, 2002)

Other

Statement

Reference

Scer\GAL4^sim.P3.7, rho^PΔ38, spi^s.UAS has midline glial cell | ectopic phenotype

(Lanoue and Jacobs, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of csw::Src64B^{src90.Scer\UAS} under the control of Scer\GAL4^twi.PG significantly suppresses the loss of VA2 muscle precursor cells seen in rho^PΔ38 embryos (69% of hemisegments have VA2 cells).

(Johnson Hamlet and Perkins, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

rho²⁰⁷

(Myat et al., 2005)

Partially rescued by

rho^PΔ38 is partially rescued by rho^hs.PSt

(Lanoue and Jacobs, 1999)

Not rescued by

rho^PΔ38 is not rescued by rho^UAS.cXa/Scer\GAL4^sim.P3.7

(Lanoue and Jacobs, 1999)

Comments

The addition of rho^Scer\UAS.cXa driven by Scer\GAL4^sim.P3.7 to rho^PΔ38 embryos is not sufficient to rescue the loss of midline glial cell phenotype seen in these mutants. The ubiquitous expression of rho^hs.PSt under heatshock is sufficient to suppress the loss of midline glial cell seen in rho^PΔ38 embryos to wild-type.

(Lanoue and Jacobs, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Fails to complement rho^7M43.

(Freeman et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

rho-1

(Yogev et al., 2010)

rho-1^PΔ38

(Wasserman et al., 2000)

rho³⁸

(Lanoue and Jacobs, 1999, Stemerdink and Jacobs, 1997)

rho^P38

(Sonnenfeld and Jacobs, 1995, Sonnenfeld and Jacob, 1994)

rho^PΔ38

rho^d38

(Matsuda et al., 2015)

rho^Δ38

(Myat et al., 2005, Inbal et al., 2004, Reich and Shilo, 2002, Johnson Hamlet and Perkins, 2001, Golembo et al., 1999, Gabay et al., 1997, Gabay et al., 1997, Wappner et al., 1997, Golembo et al., 1996, Schweitzer et al., 1995)

rho^Δp38

(Yogev et al., 2010)

rhomboid^delta38

(Melen et al., 2005)

ve^rho-PΔ38

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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