FlyBase Allele Report: Dmel\ptc[H84]

General Information

Symbol

Dmel\ptc^H84

Species

D. melanogaster

Name

FlyBase ID

FBal0035473

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

P{A92}ptc^H84

Carried in Construct

Also Known As

Ptc-lacZ, H84 ptc-lacZ

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bhat, 1996, Hooper, 1994)

Mutagen

P-element activity

(Zhang et al., 1996, Hooper, 1994, Ingham et al., 1991)

Progenitor genotype

Associated Insertion(s)

P{A92}ptc^H84

Cytology

Description

P{lacZ} has inserted into the 3' exon of ptc.

(Alcedo et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{A92}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage (with ptc^tuf-1)

(Thomas and Ingham, 2003)

abnormal size | third instar larval stage (with ptc^hdl)

(Shyamala and Bhat, 2002)

decreased occurrence of cell division | third instar larval stage (with ptc^hdl)

(Shyamala and Bhat, 2002)

lethal

(Zhang et al., 1996)

visible (with ptc⁹)

(Xia et al., 2012)

Phenotype Manifest In

adult head bristle (with ptc^559.1)

(Thomas and Ingham, 2003)

adult head bristle (with ptc^tuf-1)

(Thomas and Ingham, 2003)

adult head bristle | ectopic (with ptc^559.1)

(Thomas and Ingham, 2003)

adult head bristle | ectopic (with ptc^tuf-1)

(Thomas and Ingham, 2003)

adult head capsule (with ptc^559.1)

(Shyamala and Bhat, 2002)

adult head capsule (with ptc^hdl)

(Shyamala and Bhat, 2002)

eye (with ptc^tuf-1)

(Thomas and Ingham, 2003)

eye-antennal disc (with ptc^hdl)

(Shyamala and Bhat, 2002)

frons (with ptc^tuf-1)

(Thomas and Ingham, 2003)

fronto-orbital plate (with ptc^tuf-1)

(Thomas and Ingham, 2003)

larval EL neuron

(Deshpande et al., 2001)

neuroblast NB4-2

(Bhat, 1996)

neuroblast NB7-3 | ectopic

(Deshpande et al., 2001)

ocellar triangle (with ptc^tuf-1)

(Thomas and Ingham, 2003)

wing (with ptc⁹)

(Xia et al., 2012)

Detailed Description

Statement

Reference

ptc^tuf-1/ptc^H84 flies have mildly reduced eyes but a highly enlarged head vertex. 90% of these flies have outgrowths of head cuticle and large numbers of missing, misplaced or ectopic head bristles with the remaining 10% having no outgrowths and one or 2 displaced or missing bristles. ptc^559.1/ptc^H84 flies have normal sized eyes and head vertex and a low incidence of ectopic or misplaced head bristles.

(Thomas and Ingham, 2003)

100% of ptc^hdl/ptc^H84 transheterozygotes show head capsule defects. 4% of ptc^H84/ptc^559.1 transheterozygotes show head capsule defects.

(Shyamala and Bhat, 2002)

An NB 7-3-like fate is formed ectopically in 50% of mutant hemineuromeres. 60% of EL cells are missing.

(Deshpande et al., 2001)

30% hemisegments exhibit failure of neuroblast NB4-2 to delaminate from the neuroectoderm.

(Bhat, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ptc^H84/ptc⁹ has visible phenotype, enhanceable by Usp8^UAS.Tag:FLAG/Scer\GAL4^Bx-MS1096

(Xia et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

ptc^H84/ptc⁹ has wing phenotype, enhanceable by Usp8^UAS.Tag:FLAG/Scer\GAL4^Bx-MS1096

(Xia et al., 2012)

ptc^559.1/ptc^H84 has adult head capsule phenotype, enhanceable by smo¹

(Shyamala and Bhat, 2002)

Suppressed by

Statement

Reference

ptc^hdl/ptc^H84 has adult head capsule phenotype, suppressible by babo^Q302D.UAS/Scer\GAL4^69B

(Shyamala and Bhat, 2002)

ptc^H84 has neuroblast NB4-2 phenotype, suppressible by Df(2R)gsb

(Bhat, 1996)

NOT suppressed by

Statement

Reference

ptc^hdl/ptc^H84 has adult head capsule phenotype, non-suppressible by Df(3L)H99

(Shyamala and Bhat, 2002)

ptc^hdl/ptc^H84 has adult head capsule phenotype, non-suppressible by smo¹

(Shyamala and Bhat, 2002)

Other

Statement

Reference

babo³², ptc^H84 has adult head capsule phenotype

(Shyamala and Bhat, 2002)

babo^k07737, ptc^H84, smo¹ has adult head capsule phenotype

(Shyamala and Bhat, 2002)

babo^k07737, ptc^H84, smo¹/smo[+] has adult head capsule phenotype

(Shyamala and Bhat, 2002)

ptc^H84, smo¹ has adult head capsule phenotype

(Shyamala and Bhat, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of smo¹ to ptc^H84/ptc^559.1 animals produces an enhancement of the head capsule defect phenotype. 35% of animals exhibit the phenotype, compared to 4%. The addition of smo¹ to ptc^H84/ptc^hdl animals has no effect on the head capsule defect phenotype. All animals continue to show the phenotype. 2% of ptc^H84, smo¹/+ animals show a head capsule defect. 5% of babo³², ptc^H84 double heterozygotes exhibit head capsule defects. 10% of ptc^H84, smo¹/+, babo^k07737 animals exhibit head capsule defects. No ptc^H84, put¹⁰⁴⁶⁰ animals exhibit head capsule defects. The addition of Df(3L)H99 of ptc^H84/ptc^hdl does not suppress head capsule defects. The addition of babo^{Q302D.Scer\UAS} (driven by Scer\GAL4^69B) suppresses the head capsule defect phenotype seen in ptc^H84/ptc^hdl. None of these animals exhibit head capsule defects.

(Shyamala and Bhat, 2002)

Simultaneous elimination of gsb, Df(2R)gsb, rescues the RP2 lineage in 70% of hemisegments.

(Bhat, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ptc^k02507

(Prokopenko et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ptc^H84

(Bhat, 2017, Xia et al., 2012, Gazi et al., 2009, Merianda et al., 2005)

ptc^H84lacZ

(Thomas and Ingham, 2003)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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