FlyBase Allele Report: Dmel\neb[k05702]

General Information

Symbol

Dmel\neb^k05702

Species

D. melanogaster

Name

FlyBase ID

FBal0043429

Feature type

allele

Associated gene

Dmel\neb

Associated Insertion(s)

P{lacW}neb^k05702

Carried in Construct

Also Known As

l(2)k05702

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Ruden et al., 1997)

Mutagen

P-element activity

(Alphey et al., 1997, Ohkura et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}neb^k05702

Cytology

Description

Insertion of a P{lacW} element within the intron.

(Alphey et al., 1997)

Insertion of a P{lacW} element into the intron. The site of P{lacW} element insertion is identical to the site of insertion of the P{lacW} elements in neb^k06334.

(Ohkura et al., 1997)

P{lacW} insertion at position 9879bp.

(Ruden et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:20,085,112..20,085,112 [+]

(Spradling et al., 1999, Alphey et al., 1997, Ohkura et al., 1997, Ruden et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | recessive

(Ruden et al., 1997)

abnormal mitotic cell cycle

(Ruden et al., 1997)

lethal (with Df(2L)BSC258)

(Kahsai and Cook, 2018)

lethal | recessive

(Spradling et al., 1999, Alphey et al., 1997)

partially lethal - majority die | recessive

(Ruden et al., 1997)

some die during larval stage | recessive

(Ruden et al., 1997)

Phenotype Manifest In

interommatidial bristle

(Ruden et al., 1997)

macrochaeta

(Ruden et al., 1997)

wing

(Ruden et al., 1997)

Detailed Description

Statement

Reference

Only a small percentage of homozygotes survive to adulthood. These adult flies have a range of morphological defects. Larval neuroblasts contain polyploid figures.

(Ohkura et al., 1997)

Escapers have many missing bristles, behavioural defects and wings held out perpendicular to the body.

(Ruden et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

neb^k05702 is a suppressor of phenotype of kni^unspecified

(Ruden et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

neb⁰³⁵⁵²

(BDGP Project Members, 1994-1999)

neb⁰⁵²¹⁷

(BDGP Project Members, 1994-1999)

neb^k02406

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10391

y¹ w^67c23; P{lacW}neb^k05702/CyO

Kyoto

111083

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}neb^k05702 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

Comments

Reversion analysis indicates that the adult and cell division phenotypes of the neb^k05702 chromosome are caused by the P{lacW} element insertion. fok^HN237 does not rescue the phenotype of flies homozygous for the neb^k05702 mutation, indicating that the phenotypes associated with this mutation are due to the P{lacW} insertion disrupting the neb gene, and are not due to disruption of the fok gene.

(Ohkura et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

57/2

(Ohkura et al., 1997)

Klp38B^k05702

klp38B⁵⁷⁰²

(Ruden et al., 1997)

l(2)k05702

(Butler, 2001, Butler et al., 2001, Spradling et al., 1999, Alphey et al., 1997)

neb^k05702

(Hasan et al., 2024, Kahsai and Cook, 2018)

Name Synonyms

Secondary FlyBase IDs

References (12)

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