Overexpression of Fas2^Scer\UAS.cLa by Scer\GAL4^Tab2-201Y results in significant axon (but not dendrite) pruning defects.

Scer\GAL4^Rapgap1-OK6-mediated expression of Fas2^Scer\UAS.cLa induces a significant increase in NMJ synaptic bouton number.

Scer\GAL4^elav.PU Fas2^Scer\UAS.cLa embryos display a high degree of ISNb hyperfasciculation.

Expression of Fas2^UAS.cLa under the control of Scer\GAL4^c739 results in defects in mushroom body lobes in some adults; this can include lobes extending in the wrong direction, thicker lobes, thinner lobes, missing lobes and ectopic masses of axons at the tip of the peduncle, none of these phenotypes occur in control animals. Expression of Fas2^UAS.cLa under the control of Scer\GAL4^c739 does not affect mushroom body lobe structure in the larva, but can cause defects in the peduncle; during early metamorphosis, some alpha' lobes collapse, this is correlated with misdirection of the alpha lobe and does not occur in controls. Expression of Fas2^UAS.cLa under the control of Scer\GAL4^Tab2-201Y does not lead to mushroom body lobe defects in the larva or the adult. Expression of Fas2^UAS.cLa under the control of Scer\GAL4^NP2082 does not lead to mushroom body lobe defects in the larva or the adult. Expression of Fas2^UAS.cLa under the control of Scer\GAL4^ey-OK107 leads to a lack of extension of any mushroom body lobes (including gamma, alpha', beta', alpha and beta) in any direction from the peduncle and the formation of a mass of axons at the tip of the peduncle in the adult brain. In the larva, expression of Fas2^UAS.cLa under the control of Scer\GAL4^ey-OK107 sometimes results in a thinner dorsal lobe or a shift of the core of the peduncle closer to the border of the peduncle.

When Fas2^Scer\UAS.cLa is driven by Scer\GAL4^elav-C155, severe morphological abnormalities are seen in the third instar mushroom bodies. Both the dorsal and medial lobes are markedly affected. The core is also disrupted. When Fas2^Scer\UAS.cLa is driven by Scer\GAL4^OK107, developmental defects are seen in the majority of mushroom bodies. When Fas2^Scer\UAS.cLa is driven by Scer\GAL4^Tab2-201Y, no mushroom body defects are seen. When Fas2^Scer\UAS.cLa is driven by Scer\GAL4^238Y, only mild defects are seen in mushroom bodies. Medial lobes terminate in single blob but internally harbour three branches.

Embryos expressing Fas2^Scer\UAS.cLa under the control of Scer\GAL4^elav-C155 show modest hyperfasciculation defects in the intersegmental nerve b (ISNb).

Scer\GAL4^how-24B-mediated expression promotes muscle-muscle adhesion in vivo at a high level. Extensive muscle-muscle adhesion between subsets of ventral muscles causes defects in innervation, SNb axons reach the target region normally but the terminal abors on the internal muscle surface are greatly reduced. No abnormality in SNb axon trajectory is seen, they enter the ventral muscle field at the correct choice point and extend toward the target region normally.

Extra bristles in the vicinity of the postvertical bristles and other regions of the adult fly.

In a P{GawB}elav^C155 background the SNb axons are dramatically affected, showing bypass, stall and detour phenotypes. SNa axons are also affected, showing predominantly stall mutant phenotypes. The ISN also shows a misrouting phenotype. Axons extend from below muscle 12 to synapse on the outside, dorsal surface of the fiber: the "reach-back" phenotype In a P{GawB}C38 background causes extensive ISN growth cone exploration of and adhesive contact to the tracheal cells which is never observed in wild type. The ISN can extend past the main tracheal trunk and the contacts are withdrawn in late stage 16.

Scer\GAL4^ftz.ng expression causes a novel gain of function FN3/MP1 fused pathway phenotype at stage 16 (fused phenotype is not continuous but rather occurs for short stretches) and fused vMP2 and MP1 pathways at stage 14.

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of larval VO2 motor neuron phenotype of Sema1a^k13702

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of RP3 neuron & synapse phenotype of Sema1a^k13702

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of larval VO1 motor neuron phenotype of Sema1a^k13702

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of larval RP5 motor neuron phenotype of Sema1a^k13702

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of larval abdominal segmental nerve phenotype of Sema1a^k13702

Scer\GAL4^elav-C155/Fas2^UAS.PEST- is an enhancer of larval intersegmental nerve phenotype of Sema1a^k13702