FlyBase Allele Report: Dmel\msi[2]

General Information

Symbol

Dmel\msi²

Species

D. melanogaster

Name

FlyBase ID

FBal0050165

Feature type

allele

Associated gene

Dmel\msi

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Nakamura et al., 1994)

Progenitor genotype

P{PZ}msi⁹⁰⁰

(Nakamura et al., 1994)

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

macrochaeta & scutum

(Nakamura et al., 1994)

male germline stem cell (with msi¹)

(Siddall et al., 2012)

microchaeta & scutum

(Nakamura et al., 1994)

testis

(Siddall et al., 2006)

testis (with msi¹)

(Siddall et al., 2006)

testis sheath muscle cell

(Siddall et al., 2006)

testis sheath muscle cell (with msi¹)

(Siddall et al., 2006)

Detailed Description

Statement

Reference

msi² and msi¹/msi² mutant testes exhibit fewer brightly stained cells at the apex of the testis, indicative of a loss of early germ cells. msi² mutant testis also exhibit a swelling of the apical region in ~19% of testes analyzed, although this could be attributed to defects in the development of the outer muscle sheath of the testis.

(Siddall et al., 2006)

Homozygotes survive over two weeks post-eclosion. Microchaetae on the notum have variable phenotype but not as severe as having up to 4 sockets and no shaft. Macrochaetae also display variability.

(Nakamura et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

msi[+]/msi² is an enhancer of visible | dominant phenotype of N^55e11

(Kankel et al., 2007)

NOT Enhancer of

Statement

Reference

msi² is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

NOT Suppressor of

Statement

Reference

msi² is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

msi²/msi¹ has male germline stem cell phenotype, non-enhanceable by Rbp6¹

(Siddall et al., 2012)

NOT suppressed by

Statement

Reference

msi²/msi¹ has male germline stem cell phenotype, non-suppressible by Rbp6¹

(Siddall et al., 2012)

Enhancer of

Statement

Reference

msi² is an enhancer of egg phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

msi² is an enhancer of egg chorion phenotype of Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C}

(Geng and MacDonald, 2007)

msi[+]/msi² is an enhancer of wing phenotype of N^55e11

(Kankel et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

A msi² background very weakly enhances the dorsalisation phenotype observed in Imp^Scer\UAS.cGa overexpression (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) mutants.

(Geng and MacDonald, 2007)

Dominantly enhances the Df(1)N-54l9/+ and N^55e11/+ wing nicking phenotypes.

(Kankel et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

msi² is rescued by msi^+t14.3

(Nakamura et al., 1994)

Comments

Images (0)