FlyBase Allele Report: Dmel\Imp[UAS.cGa]

General Information

Symbol

Dmel\Imp^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Geng

FlyBase ID

FBal0217544

Feature type

allele

Associated gene

Dmel\Imp

Associated Insertion(s)

Carried in Construct

P{UAS-Imp.G}

Key Links

Transgenic product class

wild_type

(Geng and MacDonald, 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Geng and MacDonald, 2007)

Mutagen

in vitro construct

(Geng and MacDonald, 2007)

Progenitor genotype

Carried in construct

P{UAS-Imp.G}

Cytology

Description

UAS regulatory sequences drive expression of Imp coding sequences.

(Geng and MacDonald, 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Imp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR16B06

(Munroe and Doe, 2023)

increased cell number | adult stage, with Scer\GAL4^GMR16B06

(Munroe and Doe, 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Imp^Scer\UAS.cGa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second) results in a very weakly dorsalised phenotype. Approximately 78% of the eggshells appear wild-type, while the rest are moderately (18%) or strongly (4%) dorsalised.

(Geng and MacDonald, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by sqd[+]/sqd^ix50

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by sqd[+]/sqd^ix50

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by mub⁰⁴⁰⁹³/mub[+]

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by mub⁰⁴⁰⁹³/mub[+]

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by CycE^AR95/CycE[+]

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by CycE^AR95/CycE[+]

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by CycE[+]/CycE⁰⁵²⁰⁶

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by CycE[+]/CycE⁰⁵²⁰⁶

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by E2f[+]/E2f1⁰⁷¹⁷²

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by E2f[+]/E2f1⁰⁷¹⁷²

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by jigr1^BG00794

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by jigr1^BG00794

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by l(2)SH1919^SH1919

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by l(2)SH1919^SH1919

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by sna¹⁸

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by sna¹⁸

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by msi¹

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by msi¹

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg phenotype, enhanceable by msi²

(Geng and MacDonald, 2007)

Imp^UAS.cGa, Scer\GAL4^{VP16.mat.αTub67C} has egg chorion phenotype, enhanceable by msi²

(Geng and MacDonald, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

A sqd^ix50 heterozygous background enhances the dorsalised phenotypes observed when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second). Only 15% of eggshells are phenotypically normal, with 27% weakly dorsalised and 58% strongly dorsalised.

l(2)SH1919^SH1919 is a moderate enhancer of the Imp^Scer\UAS.cGa (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) dorsalisation phenotype.

sna¹⁸ is a moderate enhancer of the Imp^Scer\UAS.cGa (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) dorsalisation phenotype.

A msi¹ background weakly enhances the dorsalisation phenotype observed in Imp^Scer\UAS.cGa overexpression (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) mutants.

A msi² background very weakly enhances the dorsalisation phenotype observed in Imp^Scer\UAS.cGa overexpression (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) mutants.

A heterozygous mub⁰⁴⁰⁹³ background moderately enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A heterozygous CycE^AR95 background strongly enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A heterozygous CycE⁰⁵²⁰⁶ background strongly enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A heterozygous E2f⁰⁷¹⁷² background strongly enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A jigr1^BG00794 background very weakly enhances the dorsalisation phenotype observed in Imp^Scer\UAS.cGa overexpression (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) mutants.

Expression of Imp^Scer\UAS.cGa under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second) in a Df(3R)Exel6164, Df(3R)Exel6165, or Df(3R)Exel7316 background results in an increase in the number of embryos that exhibit dorsal appendages that are fused or very close at the base (approximately 33% compared to <1%), or are fused but slightly expanded (26%). The remaining eggs are wild-type (36%), or have either moderately or strongly dorsalised eggshells (5%).

A Df(3L)Exel6137 background moderately enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A Df(3L)ED4978 background moderately enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A Df(2L)Exel7063 background enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

A Df(3R)Exel6186 background strongly enhances the dorsalisation phenotype seen when Imp^Scer\UAS.cGa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (on the second).

(Geng and MacDonald, 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

93386

P{UAS-Imp.G}39.1, w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Imp^Scer\UAS.cGa

Imp^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Geng

Secondary FlyBase IDs

References (3)

Report Sections

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