FlyBase Allele Report: Dmel\pyd[C5]

General Information

Symbol

Dmel\pyd^C5

Species

D. melanogaster

Name

FlyBase ID

FBal0059957

Feature type

allele

Associated gene

Dmel\pyd

Associated Insertion(s)

P{lacZ.ry⁺}pyd^C5

Carried in Construct

Also Known As

P{lacZ-un1}pyd^C5

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Jung et al., 2006)

Mutagen

Delta2-3 transposase

(Chen et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lacZ.ry⁺}pyd^C5

Cytology

Description

The P{lacZ-un1} insertion site is 7 bp upstream of the pyd transcription start.

(Wei and Ellis, 2001)

Molecular lesion maps to the transcription unit previously described as 'tamou'.

(Ellis, 1998.6.17)

Insertion of a P-element.

(Chen et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{lacZ.ry⁺}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:8,846,295..8,846,295 [-]

(Choi et al., 2011, Chen et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Chen et al., 1996)

viable

(Jung et al., 2006)

visible (with pyd^tam)

(Djiane et al., 2011)

Phenotype Manifest In

dorsal tracheal branch primordium

(Jung et al., 2006)

dorsal tracheal branch primordium | somatic clone

(Jung et al., 2006)

dorsocentral bristle | increased number (with pyd^tam)

(Djiane et al., 2011)

embryonic/first instar larval cuticle

(Takahashi et al., 1998)

embryonic dorsal branch & cell-cell adherens junction | somatic clone

(Jung et al., 2006)

ganglionic tracheal branch primordium

(Jung et al., 2006)

macrochaeta

(Chen et al., 1996)

presumptive embryonic/larval tracheal system

(Jung et al., 2006)

presumptive embryonic/larval tracheal system (with Df(3R)p-XT103)

(Jung et al., 2006)

terminal tracheal cell

(Jung et al., 2006)

tracheal fusion cell

(Jung et al., 2006)

tracheal fusion cell | somatic clone

(Jung et al., 2006)

tracheal lateral trunk primordium

(Jung et al., 2006)

Detailed Description

Statement

Reference

70% of pyd^tam/pyd^C5 flies display more than four dorsocentral macrochaetae.

(Djiane et al., 2011)

No significant bristle or eye phenotypes are observed in pyd^C5 homozygotes.

(Seppa et al., 2008)

pyd^C5 embryos display defects in tracheal development including dorsal branch fusion problems, ectopic terminal cells, a lack of fusion cells, and ganglionic branch outgrowth defects. The dorsal branch phenotype is not fully penetrant; 14% of embryos show wild-type tracheal systems, 22% show defects in 1 metamere, 25% show defects in 2-4 metameres and 39% show defects in >5 metameres. Fusion defects are occasionally observed in the lateral trunk, although the penetrance of this is lower than for the dorsal branch.

pyd^C5/Df(3R)p-XT103 transheterozygotes show the same tracheal phenotypes as pyd^C5 homozygotes.

pyd^C5 mutant clones which affect the terminal cells do not affect branch fusion to a significant extent, whereas pyd^C5 clones that affect the fusion cells are associated with branch fusion defects and rarely show a wild-type branch fusion pattern. Dorsal branches with pyd^C5 clones show large loops of intercellular adherens junctions, indicating that cell intercalation is not complete. These intercalation defects are not fully penetrant. In most cases, dorsal branches with a cell intercalation phenotype are still able to stretch and eventually reach the dorsal midline, and some can even undergo branch fusion.

(Jung et al., 2006)

pyd^C5/Df(3R)p819 embryos have a dorsal open phenotype.

(Takahashi et al., 1998)

Homozygous lethal and lethal in trans with Df(3R)p819 or Df(3R)pydC2. pyd¹/pyd^C5, pyd^J17/pyd^C5, pyd^J4/pyd^C5, pyd^J2/pyd^C5, pyd^G18/pyd^C5, pyd^G7/pyd^C5 and pyd^J14/pyd^C5 flies have extra macrochaetae on the head and notum compared to wild-type.

(Chen et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

pyd^C5/pyd^tam has dorsocentral bristle | increased number phenotype, enhanceable by N[+]/N^55e11

(Djiane et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

100% of N^55e11/+; pyd^tam/pyd^C5 flies display more than four dorsocentral macrochaetae.

(Djiane et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pyd^AZ63

(Ghabrial et al., 2011)

pyd^PA14

(Ghabrial et al., 2011)

pyd^J17

(Chen et al., 1996)

Rescued by

pyd^C5/pyd^tam is rescued by pyd^UAS.GFP/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5/pyd^tam is rescued by Scer\GAL4^sca-537.4/pyd^{ΔPDZ1.UAS.GFP}

(Djiane et al., 2011)

pyd^C5/pyd^J14 is rescued by pyd^hs.PT/pyd^6-.hs

(Wei and Ellis, 2001)

pyd^C5 is rescued by pyd^hs.PT

(Ellis, 1998.6.17)

Partially rescued by

pyd^C5/pyd^tam is partially rescued by pyd^{ΔPro-Rich.UAS.GFP}/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5/pyd^tam is partially rescued by pyd^{ΔSH3.UAS.GFP}/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5 is partially rescued by Scer\GAL4^btl.PS/pyd^UAS.cJa

(Jung et al., 2006)

pyd^C5 is partially rescued by pyd^UAS.EGFP/Scer\GAL4^btl.PS

(Jung et al., 2006)

pyd^C5/pyd^J14 is partially rescued by pyd^hs.PT

(Wei and Ellis, 2001)

pyd^C5/pyd^J14 is partially rescued by pyd^6-.hs

(Wei and Ellis, 2001)

Not rescued by

pyd^C5/pyd^tam is not rescued by pyd^{ΔEx6.UAS.GFP}/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5/pyd^tam is not rescued by Scer\GAL4^sca-537.4/pyd^{ΔPDZ2.UAS.GFP}

(Djiane et al., 2011)

pyd^C5/pyd^tam is not rescued by pyd^{ΔPDZ3.UAS.GFP}/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5/pyd^tam is not rescued by pyd^{ΔGUK.UAS.GFP}/Scer\GAL4^sca-537.4

(Djiane et al., 2011)

pyd^C5 is not rescued by pyd^6-.hs

(Wei and Ellis, 2001)

Comments

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is reduced when pyd^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is reduced when pyd^{ΔPDZ1.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pyd^{ΔPDZ2.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pyd^{ΔPDZ3.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pyd^{ΔGUK.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is not reduced when pyd^{ΔEx6.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4. A more severe bristle phenotype is observed in these animals.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is partially reduced when pyd^{ΔSH3.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

The number of pyd^tam/pyd^C5 flies displaying an increased number of dorsocentral macrochaete is partially reduced when pyd^{ΔPro-Rich.Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^sca-537.4.

(Djiane et al., 2011)

Expression of either pyd^{Scer\UAS.T:Avic\GFP-EGFP} or pyd^Scer\UAS.cJa under the control of Scer\GAL4^btl.PS partially rescues the tracheal phenotype of pyd^C5 embryos.

(Jung et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Order of alleles based on increasing severity of phenotype when hemizygous: pyd¹ < pyd^J17 < pyd^J4 = pyd^J2 < pyd^G18 = pyd^G7 < pyd^J14 = pyd^C5. The extra bristle phenotype and lethality of pyd^C5 revert simultaneously in the presence of a source of P-element transposase, suggesting that both phenotypes are due to insertion of the P-element. However, a lethal mutation that fails to complement the lethality of pyd^C5, but complements the bristle phenotype of pyd^J14 has been isolated, suggesting that the P-element insertion in pyd^C5 may affect both pyd and an independent locus.

(Chen et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pyd^C5

(Choi et al., 2011, Djiane et al., 2011, Ghabrial et al., 2011, Goossens et al., 2011, Seppa et al., 2008, Jung et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (10)

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