FlyBase Allele Report: Dmel\mys[b9]

General Information

Symbol

Dmel\mys^b9

Species

D. melanogaster

Name

FlyBase ID

FBal0062549

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

heat sensitive hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

heat sensitive hypomorphic allele - genetic evidence

(Beumer et al., 1999)

Mutagen

ethyl methanesulfonate

(Jannuzi et al., 2004)

Progenitor genotype

Cytology

Description

May contain regulatory mutation as lesion not found within exons or splice sites of mys.

(Jannuzi et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

bouton

(Beumer et al., 2002)

bouton & neuromuscular junction

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal lateral longitudinal muscle 1

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3

(Beumer et al., 1999)

neuromuscular junction

(Beumer et al., 2002, Beumer et al., 1999)

neuromuscular junction & abdominal lateral longitudinal muscle 1

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 3

(Beumer et al., 1999)

wing

(Prout et al., 1997)

Detailed Description

Statement

Reference

Mutant synapses at the larval neuromuscular junction show profound undergrowth, with a reduced number of small, tightly spaced type I boutons and an increased number of satellite boutons.

(Beumer et al., 2002)

Shows incomplete late larval lethality at 29^oC. Arboreal branches of the neuromuscular junctions (NMJs) of muscle 4 are dramatically shortened in homozygous larvae and synaptic boutons are both smaller and more tightly spaced than in wild type, greatly reducing the synaptic terminal area. Mutant terminals contain abundant clusters of "mini-boutons"; very small boutons that are only occasionally seen in wild-type terminals. The mini-boutons are surrounded by subsynaptic reticulum (SSR) as is characteristic of wild-type type I boutons. Mature type I boutons are also significantly smaller than wild-type. The terminals of the NMJs on both muscles 4 and 12 also show a significant decrease in all orders of terminal branches, resulting in a very significant decrease in the total numbers of arboreal branches. Type I bouton numbers are very significantly reduced at the NMJs on muscles 4, 6 and 12. The area of muscles 4 and 12 is not significantly different from wild type. The neuromuscular junctions of mys^b9/mys¹¹ larvae contain small, tightly clustered boutons surrounded by numerous mini-boutons. Homozygous and mys^b9/mys¹¹ larvae show altered synapse specificity at the muscle 12 NMJ. More than 30% of NMJs show a "backbranched" phenotype; at least one arboreal branch leaves muscle 12 and branches back onto muscle 13 from a site away from the point of nerve entry onto muscle 12. 50% of mys^b9/mys¹¹ muscle 12 NMJs show a "pulled away" phenotype; boutons are suspended in the space between muscles 12 and 13 and defasciculation, or branching, occurs before the nerve has actually reached the point of insertion on the muscle. SSR morphology appears qualitatively unaffected in type I NMJs of mys^b9/mys¹¹ larvae and the SSR mean cross-section area is not significantly different from wild type. Boutons appear ultrastructurally normal and the presynaptic membranes are normally attached to the postsynaptic membrane. Synaptic vesicle density is indistinguishable from wild type. At the muscle 4 NMJ, most homozygous larvae have nerve-evoked excitatory junctional current (EJC) amplitudes in the normal range, while some have unusually small EJC amplitudes. Overall, the mean EJC amplitude is only slightly reduced compared to wild type. EJC amplitudes are similarly unaffected in mys^b9/mys¹¹ larvae. At the muscle 6 NMJ, homozygous larvae have a significantly decreased EJC amplitude, while mys^b9/mys¹¹ larvae appear normal. The frequency of miniature EJCs is normal at the NMJ, and the mean (quantal) amplitude is very slightly increased in homozygous larvae.

(Beumer et al., 1999)

Wing blisters are seen in approximately 1-2% of hemizygotes, and approximately 60-80% of hemizygotes have held-out wings.

(Prout et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mys^b9 has visible | recessive phenotype, enhanceable by stck[+]/stck^3R-17

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by stck[+]/stck^unspecified

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by Delta^unspecified/Dl[+]

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by bs[+]/bs^2R-19

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by bs[+]/bs^unspecified

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by rhea[+]/rhea¹

(Prout et al., 1997)

mys^b9 has visible | recessive phenotype, enhanceable by rhea[+]/rhea²

(Prout et al., 1997)

Suppressed by

Statement

Reference

mys^b9 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^b9 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

NOT suppressed by

Statement

Reference

mys^b9 has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav-C155/CaMKII^UAS.cKa

(Beumer et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

mys^b9 has wing phenotype, enhanceable by stck[+]/stck^3R-17

(Prout et al., 1997)

mys^b9 has wing phenotype, enhanceable by rhea[+]/rhea²

(Prout et al., 1997)

mys^b9 has wing phenotype, enhanceable by stck[+]/stck^unspecified

(Prout et al., 1997)

mys^b9 has wing phenotype, enhanceable by bs[+]/bs^2R-19

(Prout et al., 1997)

mys^b9 has wing phenotype, enhanceable by bs[+]/bs^unspecified

(Prout et al., 1997)

mys^b9 has wing phenotype, enhanceable by rhea[+]/rhea¹

(Prout et al., 1997)

Suppressed by

Statement

Reference

mys^b9 has bouton phenotype, suppressible by Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^b9 has bouton phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^b9 has neuromuscular junction phenotype, suppressible by Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa

(Beumer et al., 2002)

mys^b9 has neuromuscular junction phenotype, suppressible by Scer\GAL4^e22c/CaMKII^UAS.cKa

(Beumer et al., 2002)

NOT suppressed by

Statement

Reference

mys^b9 has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^elav-C155/CaMKII^UAS.cKa

(Beumer et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Animals carrying Rat\CamKII-I^Ala.hs which are also mutant for mys^b9 show an approximately 20% increase in bouton number at the neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 has no effect on the undergrowth phenotype seen at the mys^b9 larval neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW or Scer\GAL4^e22c completely suppresses the undergrowth phenotype seen at the mys^b9 larval neuromuscular junction.

(Beumer et al., 2002)

The frequency of wing blisters in mys^b9 hemizygotes is strongly increased if the flies are also heterozygous for bs^unspecified, rhea² or rhea¹. The frequency of the wings held-out phenotype is enhanced if the flies are also heterozygous for stck^unspecified, stck^3R-17, rhea¹ or rhea². Shows a weak genetic interaction with Dl^unspecified.

(Prout et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mys^G1

(Jannuzi et al., 2004)

mys^xR04

(Jannuzi et al., 2004)

Rescued by

mys^b9 is rescued by mys^UAS.cDa/Scer\GAL4^Mhc.PW

(Beumer et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that permits the survival of an F1 male.

(Jannuzi et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mys^b9

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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