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General Information
Symbol
Dmel\mysb9
Species
D. melanogaster
Name
FlyBase ID
FBal0062549
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

May contain regulatory mutation as lesion not found within exons or splice sites of mys.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

bouton & neuromuscular junction

bouton & neuromuscular junction & abdominal lateral longitudinal muscle 1

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3

neuromuscular junction & abdominal lateral longitudinal muscle 1

neuromuscular junction & abdominal ventral longitudinal muscle 1

neuromuscular junction & abdominal ventral longitudinal muscle 3

Detailed Description
Statement
Reference

Mutant synapses at the larval neuromuscular junction show profound undergrowth, with a reduced number of small, tightly spaced type I boutons and an increased number of satellite boutons.

Shows incomplete late larval lethality at 29oC. Arboreal branches of the neuromuscular junctions (NMJs) of muscle 4 are dramatically shortened in homozygous larvae and synaptic boutons are both smaller and more tightly spaced than in wild type, greatly reducing the synaptic terminal area. Mutant terminals contain abundant clusters of "mini-boutons"; very small boutons that are only occasionally seen in wild-type terminals. The mini-boutons are surrounded by subsynaptic reticulum (SSR) as is characteristic of wild-type type I boutons. Mature type I boutons are also significantly smaller than wild-type. The terminals of the NMJs on both muscles 4 and 12 also show a significant decrease in all orders of terminal branches, resulting in a very significant decrease in the total numbers of arboreal branches. Type I bouton numbers are very significantly reduced at the NMJs on muscles 4, 6 and 12. The area of muscles 4 and 12 is not significantly different from wild type. The neuromuscular junctions of mysb9/mys11 larvae contain small, tightly clustered boutons surrounded by numerous mini-boutons. Homozygous and mysb9/mys11 larvae show altered synapse specificity at the muscle 12 NMJ. More than 30% of NMJs show a "backbranched" phenotype; at least one arboreal branch leaves muscle 12 and branches back onto muscle 13 from a site away from the point of nerve entry onto muscle 12. 50% of mysb9/mys11 muscle 12 NMJs show a "pulled away" phenotype; boutons are suspended in the space between muscles 12 and 13 and defasciculation, or branching, occurs before the nerve has actually reached the point of insertion on the muscle. SSR morphology appears qualitatively unaffected in type I NMJs of mysb9/mys11 larvae and the SSR mean cross-section area is not significantly different from wild type. Boutons appear ultrastructurally normal and the presynaptic membranes are normally attached to the postsynaptic membrane. Synaptic vesicle density is indistinguishable from wild type. At the muscle 4 NMJ, most homozygous larvae have nerve-evoked excitatory junctional current (EJC) amplitudes in the normal range, while some have unusually small EJC amplitudes. Overall, the mean EJC amplitude is only slightly reduced compared to wild type. EJC amplitudes are similarly unaffected in mysb9/mys11 larvae. At the muscle 6 NMJ, homozygous larvae have a significantly decreased EJC amplitude, while mysb9/mys11 larvae appear normal. The frequency of miniature EJCs is normal at the NMJ, and the mean (quantal) amplitude is very slightly increased in homozygous larvae.

Wing blisters are seen in approximately 1-2% of hemizygotes, and approximately 60-80% of hemizygotes have held-out wings.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

mysb9 has visible | recessive phenotype, enhanceable by stck[+]/stck3R-17

mysb9 has visible | recessive phenotype, enhanceable by stck[+]/stckunspecified

mysb9 has visible | recessive phenotype, enhanceable by Dlunspecified/Dl[+]

mysb9 has visible | recessive phenotype, enhanceable by bs[+]/bs2R-19

mysb9 has visible | recessive phenotype, enhanceable by bs[+]/bsunspecified

mysb9 has visible | recessive phenotype, enhanceable by rhea[+]/rhea1

mysb9 has visible | recessive phenotype, enhanceable by rhea[+]/rhea2

Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

mysb9 has wing phenotype, enhanceable by stck[+]/stck3R-17

mysb9 has wing phenotype, enhanceable by rhea[+]/rhea2

mysb9 has wing phenotype, enhanceable by stck[+]/stckunspecified

mysb9 has wing phenotype, enhanceable by bs[+]/bs2R-19

mysb9 has wing phenotype, enhanceable by bs[+]/bsunspecified

mysb9 has wing phenotype, enhanceable by rhea[+]/rhea1

Suppressed by
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Animals carrying Rat\CamKII-IAla.hs which are also mutant for mysb9 show an approximately 20% increase in bouton number at the neuromuscular junction. Expression of CaMKIIScer\UAS.cKa under the control of Scer\GAL4elav-C155 has no effect on the undergrowth phenotype seen at the mysb9 larval neuromuscular junction. Expression of CaMKIIScer\UAS.cKa under the control of Scer\GAL4Mhc.PW or Scer\GAL4e22c completely suppresses the undergrowth phenotype seen at the mysb9 larval neuromuscular junction.

The frequency of wing blisters in mysb9 hemizygotes is strongly increased if the flies are also heterozygous for bsunspecified, rhea2 or rhea1. The frequency of the wings held-out phenotype is enhanced if the flies are also heterozygous for stckunspecified, stck3R-17, rhea1 or rhea2. Shows a weak genetic interaction with Dlunspecified.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutation that permits the survival of an F1 male.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
mysb9
Name Synonyms
Secondary FlyBase IDs
    References (5)