FlyBase Allele Report: Dmel\CaMKII[UAS.cKa]

General Information

Symbol

Dmel\CaMKII^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Koh

FlyBase ID

FBal0117905

Feature type

allele

Associated gene

Dmel\CaMKII

Associated Insertion(s)

Carried in Construct

P{UAS-CaMKII.R3}

Also Known As

UAS-CaMKII

Key Links

Transgenic product class

wild_type

(Koh et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Koh et al., 1999)

Mutagen

in vitro construct

(Koh et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-CaMKII.R3}

Cytology

Description

UASt regulatory sequences drive expression of the wild-type R3 isoform of CaMKII.

(Koh et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CaMKII

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neuromuscular disease

CEA with Hsap\TRPV4^{R269C.UAS.BID}

(Woolums et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Oka et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^c305a

(Malik et al., 2013)

abnormal memory, with Scer\GAL4^ey-OK107

(Malik et al., 2013)

abnormal neuroanatomy, with Scer\GAL4^109(2)80

(Andersen et al., 2005)

decreased cell number | larval stage, with Scer\GAL4^dome-PG125

(Destalminil-Letourneau et al., 2021)

Phenotype Manifest In

adult heart, with Scer\GAL4^tin.CΔ4

(Santalla et al., 2014)

embryonic/larval crystal cell | decreased number | larval stage, with Scer\GAL4^dome-PG125

(Destalminil-Letourneau et al., 2021)

filopodium & dendrite, with Scer\GAL4^109(2)80

(Andersen et al., 2005)

Detailed Description

Statement

Reference

The expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PU does not lead to lamina vacuolization compared to controls.

(Oka et al., 2017)

Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^tin.CΔ4 results in an increase in spontaneous heart rate, a decrease in arrhythmogenic index, and a greater fraction of flies showing asystoles; but no difference in the number of ectopic heart beats, or the number of atypical beats over total beats as compared to wild type.

(Santalla et al., 2014)

Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^VGlut-OK371 has no significant effect on bouton number/area for type Ib boutons at the larval neuromuscular junction.

(Gillespie and Hodge, 2013)

Flies expressing CaMKII^Scer\UAS.cKa under the control of either Scer\GAL4^ey-OK107, Scer\GAL4^c305a, Scer\GAL4^Mef2.247 or Scer\GAL4^CaMKII.7 show normal learning (measured as 2 minute memory) in an olfactory aversive conditioning assay.

Flies expressing CaMKII^Scer\UAS.cKa under the control of either Scer\GAL4^ey-OK107 or Scer\GAL4^c305a show severely reduced middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

Flies expressing CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mef2.247 show normal middle-term memory (measured 3 hours after one cycle of training) in an olfactory aversive conditioning assay.

Flies expressing CaMKII^Scer\UAS.cKa under the control of either Scer\GAL4^ey-OK107 or Scer\GAL4^c305a have severely reduced long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

Flies expressing CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mef2.247 show normal long-term memory (measured 24 hours after 5 cycles of spaced training) in an olfactory aversive conditioning assay.

(Malik et al., 2013)

CaMKII^Scer\UAS.cKa expression, under the regulation of Scer\GAL4^109(2)80 does not generate any detectable differences in larval dendrite structure compared to wild-type, even when two transgenes are activated.

(Andersen et al., 2005)

Expression of CaMKII^Scer\UAS.cKa under the control of either Scer\GAL4^Mhc.PW, Scer\GAL4^elav-C155 or Scer\GAL4^e22c produces no significant alteration in synaptic architecture at the larval neuromuscular junction.

(Beumer et al., 2002)

Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^unspecified at both pre- and postsynaptic sites at the larval neuromuscular junction has no significant effect.

(Koh et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CaMKII^UAS.cKa, Scer\GAL4^dome-PG125 has decreased cell number | larval stage phenotype, enhanceable by btl^RNAi.UAS.cUa, Scer\GAL4^dome-PG125

(Destalminil-Letourneau et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^Oamb.4.4/CaMKII^UAS.cKa is a suppressor of female sterile | recessive phenotype of Octβ2R^f05679

(Lim et al., 2014)

Scer\GAL4^Oamb.4.4/CaMKII^UAS.cKa is a suppressor | partially of decreased fecundity | recessive | female phenotype of Octβ2R^f05679

(Lim et al., 2014)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of abnormal neuroanatomy phenotype of mys^ts1

(Beumer et al., 2002)

Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa is a suppressor of abnormal neuroanatomy phenotype of mys^ts1

(Beumer et al., 2002)

Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa is a suppressor of abnormal neuroanatomy phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of abnormal neuroanatomy phenotype of mys^b9

(Beumer et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/CaMKII^UAS.cKa is a non-suppressor of abnormal neuroanatomy phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^elav-C155/CaMKII^UAS.cKa is a non-suppressor of abnormal neuroanatomy phenotype of mys^ts1

(Beumer et al., 2002)

Other

Statement

Reference

CaMKII^UAS.cKa, Hsap\TRPV4^{R269C.UAS.BID}, Scer\GAL4^CCAP.PU has visible | adult stage phenotype

(Woolums et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

CaMKII^UAS.cKa, Scer\GAL4^dome-PG125 has embryonic/larval crystal cell | larval stage | decreased number phenotype, enhanceable by btl^RNAi.UAS.cUa, Scer\GAL4^dome-PG125

(Destalminil-Letourneau et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^Oamb.4.4/CaMKII^UAS.cKa is a suppressor | partially of egg | decreased number phenotype of Octβ2R^f05679

(Lim et al., 2014)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of bouton phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa is a suppressor of neuromuscular junction phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of neuromuscular junction phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of bouton phenotype of mys^ts1

(Beumer et al., 2002)

Scer\GAL4^e22c/CaMKII^UAS.cKa is a suppressor of neuromuscular junction phenotype of mys^ts1

(Beumer et al., 2002)

Scer\GAL4^Mhc.PW/CaMKII^UAS.cKa is a suppressor of bouton phenotype of mys^b9

(Beumer et al., 2002)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/CaMKII^UAS.cKa is a non-suppressor of neuromuscular junction phenotype of mys^b9

(Beumer et al., 2002)

Scer\GAL4^elav-C155/CaMKII^UAS.cKa is a non-suppressor of neuromuscular junction phenotype of mys^ts1

(Beumer et al., 2002)

Other

Statement

Reference

CaMKII^UAS.cKa, Hsap\TRPV4^{R269C.UAS.BID}, Scer\GAL4^CCAP.PU has wing phenotype

(Woolums et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 has no effect on the undergrowth phenotype seen at the mys^b9 larval neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW or Scer\GAL4^e22c completely suppresses the undergrowth phenotype seen at the mys^b9 larval neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 has no effect on the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^Mhc.PW results in a slight, but insignificant, decrease in the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction, partially rescuing the overproduction of type I boutons. Expression of CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^e22c completely rescues the overgrowth phenotype seen at the mys^ts1 larval neuromuscular junction.

(Beumer et al., 2002)

Xenogenetic Interactions

Statement

Reference

Individuals co-expressing Hsap\TRPV4^{R269C.UAS.BID} and CaMKII^UAS.cKa under the control of Scer\GAL4^CCAP.PU fail to expand their wings after eclosion.

(Woolums et al., 2020)

The co-expression of CaMKII^Scer\UAS.cKa enhances the lamina vacuolization induced by the expression of Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PU.

The co-expression of CaMKII^Scer\UAS.cKa and Hsap\MAPT^{S2A.Scer\UAS.0N4R} under the control of Scer\GAL4^GMR.PU does not lead to frequent lamina vacuolization compared to controls.

(Oka et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29662

w^*; P{UAS-CaMKII.R3}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CaMKII^Scer\UAS.cKa

CaMKII^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Koh

(Koh et al., 1999)

Secondary FlyBase IDs

References (16)

Report Sections

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