FlyBase Allele Report: Dmel\mys[UAS.cDa]

General Information

Symbol

Dmel\mys^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Diamond

FlyBase ID

FBal0102506

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

P{UAS-mys.D}

Also Known As

UAS-mys, UAS-βPS

Key Links

Transgenic product class

wild_type

(Beumer et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Beumer et al., 1999)

Mutagen

in vitro construct

(Beumer et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-mys.D}

Cytology

Description

UAS regulatory sequences drive mys expression.

(Beumer et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mys

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^F11

(Beumer et al., 1999)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PH

(Han et al., 2012)

Phenotype Manifest In

bouton & neuromuscular junction, with Scer\GAL4^elav-C155

(Beumer et al., 1999)

bouton & neuromuscular junction, with Scer\GAL4^F11

(Beumer et al., 1999)

bouton & neuromuscular junction, with Scer\GAL4^α

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^F11

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^α

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^F11

(Beumer et al., 1999)

bouton & neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^α

(Beumer et al., 1999)

larval dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PH

(Han et al., 2012)

male germline stem cell, with Scer\GAL4^C587

(Issigonis et al., 2009)

neuromuscular junction, with Scer\GAL4^elav-C155

(Beumer et al., 1999)

neuromuscular junction, with Scer\GAL4^F11

(Beumer et al., 1999)

neuromuscular junction, with Scer\GAL4^α

(Beumer et al., 1999)

neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4^F11

(Beumer et al., 1999)

neuromuscular junction & abdominal lateral longitudinal muscle 1, with Scer\GAL4^α

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^F11

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 1, with Scer\GAL4^α

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^F11

(Beumer et al., 1999)

neuromuscular junction & abdominal ventral longitudinal muscle 3, with Scer\GAL4^α

(Beumer et al., 1999)

Detailed Description

Statement

Reference

Expression of mys^Scer\UAS.cDa in class IV da neurons under the control of Scer\GAL4^ppk.PH causes significant dendritic reduction.

(Han et al., 2012)

Overexpression of mys^Scer\UAS.cDa driven by Scer\GAL4^C587 in cyst cells and cyst progenitor cells reduces the number of germline stem cells per testis significantly.

(Issigonis et al., 2009)

Expression of mys^Scer\UAS.cDa under the control of Scer\GAL4^elav-C155 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of larvae. However, bouton number is normal and there is no increase in the number of mini-boutons. Expression of mys^Scer\UAS.cDa under the control of Scer\GAL4^α or Scer\GAL4^F11 results in a significant reduction in branching and bouton numbers in the neuromuscular junctions of muscles 6 and 12, and a significant reduction in branching but not bouton numbers in the neuromuscular junctions of muscle 4. A moderate increase in the number of mini-boutons is seen.

(Beumer et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PH, mys^UAS.cDa has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by mew^UAS.cMBa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Enhancer of

Statement

Reference

mys^UAS.cDa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Suppressor of

Statement

Reference

mys^UAS.cDa, mew^UAS.cMBb, Scer\GAL4^ppk.PG is a suppressor of abnormal neuroanatomy | larval stage phenotype of fne^KOZ40

(Alizzi et al., 2020)

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of abnormal neuroanatomy | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor | partially of abnormal neuroanatomy phenotype of Ret^C168/Df(2L)BSC312

(Soba et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Sin1^e03756

(Han et al., 2012)

Other

Statement

Reference

Scer\GAL4^ppk.PH, mew^UAS.cMBa, mys^UAS.cDa has abnormal neuroanatomy | third instar larval stage phenotype

(Han et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PH, mys^UAS.cDa has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by mew^UAS.cMBa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Enhancer of

Statement

Reference

mys^UAS.cDa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Buff et al., 2007)

Suppressor of

Statement

Reference

mys^UAS.cDa, mew^UAS.cMBb, Scer\GAL4^ppk.PG is a suppressor of larval multidendritic class IV neuron | larval stage phenotype of fne^KOZ40

(Alizzi et al., 2020)

mys^UAS.cDa, mew^UAS.cMBb, Scer\GAL4^ppk.PG is a suppressor of dendritic branch | larval stage phenotype of fne^KOZ40

(Alizzi et al., 2020)

mys^UAS.cDa, mew^UAS.cMBb, Scer\GAL4^ppk.PG is a suppressor of dendritic tree | larval stage phenotype of fne^KOZ40

(Alizzi et al., 2020)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PU is a suppressor of larval multidendritic class IV neuron | third instar larval stage phenotype of Sema2b^f02042/Df(2R)Sema2b-C4

(Meltzer et al., 2016)

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of larval multidendritic class IV neuron | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, Scer\GAL4^elav-C155, mys^UAS.cDa is a suppressor of dendrite | somatic clone phenotype of raw^jj102

(Lee et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor | partially of larval multidendritic class IV neuron phenotype of Ret^C168/Df(2L)BSC312

(Soba et al., 2015)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of fry⁰²²⁴⁰/fry¹

(Han et al., 2012)

mew^UAS.cMBa, mys^UAS.cDa, Scer\GAL4^ppk.PH is a suppressor of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Sin1^e03756

(Han et al., 2012)

Other

Statement

Reference

Scer\GAL4^ppk.PH, mew^UAS.cMBa, mys^UAS.cDa has larval ventral multidendritic neuron vdaB | third instar larval stage phenotype

(Han et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of mew^{Scer\UAS.cMBa} and mys^Scer\UAS.cDa under the control of Scer\GAL4^ppk.PU in the Sema-2b^f02042/Df(2R)Sema-2b-C4 mutant larvae background fully rescued the increased dendritic crossing phenotype in the class IV da neurons displayed by the Sema-2b^f02042/Df(2R)Sema-2b-C4 mutants.

(Meltzer et al., 2016)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^elav-C155 suppresses the number of dendrite-dendrite crossings seen in raw^jj102 mutant class IV dendritic arborizing neuron clones. Terminal branch number is significantly restored and a greater fraction of stable terminals are seen compared. However, the mutant terminal dendrite elongation defect is not rescued.

(Lee et al., 2015)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} in class IV dendritic arborizing neurons under the control of Scer\GAL4^ppk.PH partially suppresses the dendrite growth seen in Ret^C168/Df(2L)BSC312 mutant larvae and completely suppresses the loss of dendrite-ECM interaction. The reduction in dendritic field coverage is not suppressed.

(Soba et al., 2015)

Expression of mew^{Scer\UAS.cMBa} largely rescues the dendritic reduction seen when mys^Scer\UAS.cDa is expressed in class IV da neurons under the control of Scer\GAL4^ppk.PH.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} under the control of Scer\GAL4^ppk.PH completely eliminates the enclosure of ventral midline vdaB neurons in the epidermis. 8.43% of vdaB neurons are enclosed in wild type. All associated non-contacting dendritic crossing is also suppressed.

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} the control of Scer\GAL4^ppk.PH rescues the increase in epidermally enclosed dendrites seen in fry¹/fry⁰²²⁴⁰ mutants. The dendritic crossing seen in fry¹/fry⁰²²⁴⁰ mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrites, rather than non-contacting crossings between an epidermally enclosed dendrite and one attached to the ECM. The crossing on the v'ada/vdaB interface is also mostly rescued and all non-contacting crossing is eliminated.

(Han et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

mys^UAS.cDa/Scer\GAL4^Mhc.PW rescues mys^b9

(Beumer et al., 2002)

Fails to rescue

mys^UAS.cDa/Scer\GAL4^Mhc.PW fails to rescue mys^ts1

(Beumer et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

M. Diamond

(Beumer et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mys^Scer\UAS.cDa

mys^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Diamond

(Beumer et al., 1999)

Secondary FlyBase IDs

References (13)

Report Sections

Open Close