FlyBase Allele Report: Dmel\fry[1]

General Information

Symbol

Dmel\fry¹

Species

D. melanogaster

Name

FlyBase ID

FBal0126392

Feature type

allele

Associated gene

Dmel\fry

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Cong et al., 2001)

Progenitor genotype

Cytology

Description

1bp deletion in exon 3, resulting in a frameshift after amino acid residue 403.

(Cong et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:9,670,552..9,670,554 [-]

Comment:

A 1bp deletion in exon 3, resulting in a frameshift after amino acid residue 403. The deletion was annotated at the first base of codon 404 of fry-PB. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Cong et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of syndromic intellectual disability

CEA

(Pan et al., 2024)

CEA with fry^F2024L.RMCE

(Pan et al., 2024)

CEA with fry^Y3410C.RMCE

(Pan et al., 2024)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with fry^F2024L.RMCE)

(Pan et al., 2024)

abnormal developmental rate (with fry^Y3410C.RMCE)

(Pan et al., 2024)

abnormal neuroanatomy | third instar larval stage (with fry⁰²²⁴⁰)

(Han et al., 2012)

abnormal neurophysiology | adult stage (with fry^F2024L.RMCE)

(Pan et al., 2024)

cell lethal | adult stage | somatic clone - tissue specific

(Pan et al., 2024)

female sterile (with fry²), with Scer\GAL4^Act.PU, fry^{UAS.Tag:MYC.GFP}

(Fang et al., 2010)

lethal - all die before end of larval stage | recessive

(Cong et al., 2001)

lethal - all die before end of second instar larval stage

(Pan et al., 2024)

lethal - all die before end of second instar larval stage (with Df(3L)BSC669)

(Pan et al., 2024)

lethal - all die before end of second instar larval stage (with fry^F2746S.PE)

(Pan et al., 2024)

lethal - all die before end of second instar larval stage (with fry^F2746S.RMCE)

(Pan et al., 2024)

lethal - all die before end of second instar larval stage (with fry^{MI12326-TG4.1})

(Pan et al., 2024)

majority die during first instar larval stage (with Df(3L)BSC669)

(Pan et al., 2024)

majority die during first instar larval stage (with fry^F2746S.PE)

(Pan et al., 2024)

majority die during first instar larval stage (with fry^F2746S.RMCE)

(Pan et al., 2024)

male sterile (with fry²), with Scer\GAL4^Act.PU, fry^{UAS.Tag:MYC.GFP}

(Fang et al., 2010)

some die during embryonic stage

(Pan et al., 2024)

some die during embryonic stage (with Df(3L)BSC669)

(Pan et al., 2024)

some die during embryonic stage (with fry^F2746S.PE)

(Pan et al., 2024)

some die during embryonic stage (with fry^F2746S.RMCE)

(Pan et al., 2024)

some die during embryonic stage (with fry^{MI12326-TG4.1})

(Pan et al., 2024)

some die during first instar larval stage

(Pan et al., 2024)

some die during first instar larval stage (with fry^{MI12326-TG4.1})

(Pan et al., 2024)

some die during larval stage | recessive

(Cong et al., 2001)

some die during second instar larval stage

(Pan et al., 2024)

some die during second instar larval stage (with Df(3L)BSC669)

(Pan et al., 2024)

some die during second instar larval stage (with fry^F2746S.PE)

(Pan et al., 2024)

some die during second instar larval stage (with fry^F2746S.RMCE)

(Pan et al., 2024)

some die during second instar larval stage (with fry^{MI12326-TG4.1})

(Pan et al., 2024)

viable (with fry^F2024L.PE)

(Pan et al., 2024)

viable (with fry^F2024L.RMCE)

(Pan et al., 2024)

viable (with fry^S2910I.RMCE)

(Pan et al., 2024)

viable (with fry^WT.RMCE)

(Pan et al., 2024)

viable (with fry^Y3410C.RMCE)

(Pan et al., 2024)

visible | adult stage | somatic clone - tissue specific

(Pan et al., 2024)

visible | recessive | somatic clone

(Cong et al., 2001)

Phenotype Manifest In

dendrite | cell autonomous | somatic clone | increased number

(Emoto et al., 2004)

egg (with fry²), with Scer\GAL4^Act.PU, fry^{UAS.Tag:MYC.GFP}

(Fang et al., 2010)

egg | somatic clone

(Horne-Badovinac et al., 2012)

eye | somatic clone - tissue specific

(Pan et al., 2024)

larval dorsal multidendritic neuron ddaC | third instar larval stage (with fry⁰²²⁴⁰)

(Han et al., 2012)

multidendritic neuron & dendrite

(Emoto et al., 2004)

multidendritic neuron & dendrite (with fry⁰²²⁴⁰)

(Emoto et al., 2004)

multidendritic neuron & dendrite | somatic clone

(Emoto et al., 2004)

multidendritic neuron & dendrite | supernumerary

(Emoto et al., 2004)

multidendritic neuron & dendrite | supernumerary (with fry⁰²²⁴⁰)

(Emoto et al., 2004)

ovary (with fry²), with Scer\GAL4^Act.PU, fry^{UAS.Tag:MYC.GFP}

(Fang et al., 2010)

retina | adult stage (with fry^F2024L.RMCE)

(Pan et al., 2024)

wing hair | adult stage | increased number | somatic clone - tissue specific

(Pan et al., 2024)

wing hair | somatic clone | increased number

(Cong et al., 2001)

Detailed Description

Statement

Reference

fry¹/fry⁰²²⁴⁰ mutant third instar larvae show a significant increase in the proportion of dorsal midline ddaC dendritic length that is enclosed within the epidermis rather than attached to the ECM. Dendrite crossing is seen in these mutants, and the majority of these crossings are between enclosed dendrites and dendrites attached to the ECM and are thus non-contacting. Unlike in wild type, crossing is also seen between dendrites on the interface between the v'ada and the vdaB neurons. These crossings are non-contacting.

As in wild type, when two fry¹ homozygous mutant ddaC dendrites approach each other they either retract or alter the direction of extension to avoid crossing. However when two dendrites cross that are in a different apical basal plane (i.e one is attached to the ECM and the other is enclosed in the epidermis) the two dendrites cross over each other.

(Han et al., 2012)

Females carrying homozygous follicle cell clones produce round eggs.

(Horne-Badovinac et al., 2012)

Expression of fry^{UAS.Tag:MYC.GFP} under the control of Scer\GAL4^Act.PU completely rescues the lethality of fry¹/fry² mutants. These rescued flies are both male and female sterile. The paired ovaries of rescued females are often of different sizes, and the eggs produced are more rounded than normal and are rarely laid. Wing cells in adult wings from these rescued flies display (in 99% of cases) a single hair compared to the more than four hairs per cell seen in fry loss of function clones.

(Fang et al., 2010)

fry¹/+ heterozygotes exhibit wild-type dendritic tiling, with wild-type levels of dendritic crossing points per υm[2].

(Emoto et al., 2006)

fry¹ mutants exhibit supernumerary terminal branching and defective dendritic tiling. their terminal branch number and the total branch length are increased by a factor of two and the branches of ddaC neurons often overlap each other. The major branch architecture appears normal. This phenotype is apparent in early first instar larvae. The tiling defect can be seen independently from the overbranching defect. The crossing branches have rigid and straight trajectories. ddaC neurons in fry⁰²²⁴⁰/fry¹ larvae exhibit tiling defects, though they have normal dendritic length and branch points. ddaC neurons in fry⁰²²⁴⁰/fry¹ larvae exhibit tiling defects, though they have normal dendritic length and branch points. When somatic clones are made in the neurons, mutant dendrites display a 50% increase in the number of branches. Mutant clones also exhibit a tiling defect. In mutants, the v'ada and vdaB dendrites often invade neighbouring fields. Major branches as well as terminal branches overlap extensively. This appears to be caused by a defective like-like dendrite avoidance response: dendrites can grow and retract normally, but their inability to turn in order to avoid like dendrites results in tiling defects.

(Emoto et al., 2004)

Most cells in homozygous clones in the wing form a cluster of hairs (average is 5.7 hairs/cell) and some hairs are split distally.

(Cong et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

fry⁰²²⁴⁰/fry¹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Other

Statement

Reference

Df(2R)Sema2b-C4, fry¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Meltzer et al., 2016)

Vang^stbm-6, fry¹/fry[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Matsubara et al., 2011)

fry¹, stan[+]/stan^E59 has abnormal neuroanatomy | third instar larval stage phenotype

(Matsubara et al., 2011)

fry¹/fry[+], hpo^MGH4 has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

fry¹, hpo^MGH4/hpo[+] has abnormal neuroanatomy phenotype

(Emoto et al., 2006)

fry¹/fry[+], mats^e03077, trc[+]/trc^S066917a has visible | dominant phenotype

(He et al., 2005)

fry¹, mwh^unspecified has visible | somatic clone phenotype

(Cong et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

fry⁰²²⁴⁰/fry¹ has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible by mew^UAS.cMBa/mys^UAS.cDa/Scer\GAL4^ppk.PH

(Han et al., 2012)

Enhancer of

Statement

Reference

fry¹/fry[+] is an enhancer of wing hair | increased number phenotype of Scer\GAL4^ap-md544, trc^S292A.UAS.L

(He et al., 2005)

fry¹/fry[+] is an enhancer of wing hair | increased number phenotype of Scer\GAL4^ap-md544, trc^T453A.UAS.L

(He et al., 2005)

fry¹/fry[+] is an enhancer of wing hair | increased number phenotype of Scer\GAL4^ap-md544, trc^{S292A+T453A.UAS.L}

(He et al., 2005)

Other

Statement

Reference

Df(2R)Sema2b-C4, fry¹ has larval multidendritic class IV neuron | third instar larval stage phenotype

(Meltzer et al., 2016)

Df(2R)Sema2b-C4, fry¹ has dendrite | third instar larval stage phenotype

(Meltzer et al., 2016)

Vang^stbm-6, fry¹/fry[+] has dendrite | third instar larval stage phenotype

(Matsubara et al., 2011)

Vang^stbm-6, fry¹/fry[+] has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Matsubara et al., 2011)

fry¹, stan[+]/stan^E59 has dendrite | third instar larval stage phenotype

(Matsubara et al., 2011)

fry¹, stan[+]/stan^E59 has larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype

(Matsubara et al., 2011)

fry¹/fry[+], hpo^MGH4 has multidendritic dendrite phenotype

(Emoto et al., 2006)

fry¹, hpo^MGH4/hpo[+] has multidendritic dendrite phenotype

(Emoto et al., 2006)

fry¹/fry[+], mats^e03077, trc[+]/trc^S066917a has wing hair | increased number phenotype

(He et al., 2005)

fry¹, mwh^unspecified has wing hair | somatic clone phenotype

(Cong et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The level of non-contacting dendrite crossing in class IV dendrite arborizing larval neurons is significantly increased in fry¹;Df(2R)Sema-2b-C4 double mutants compared to either fry¹/+ or Df(2R)Sema-2b-C4/+ heterozygotes.

(Meltzer et al., 2016)

Co-expression of mys^Scer\UAS.cDa and mew^{Scer\UAS.cMBa} the control of Scer\GAL4^ppk.PH rescues the increase in epidermally enclosed dendrites seen in fry¹/fry⁰²²⁴⁰ mutants. The dendritic crossing seen in fry¹/fry⁰²²⁴⁰ mutants is also largely rescued. The dendritic crossings that remain in the rescue flies are between two ECM attached dendrites, rather than non-contacting crossings between an epidermally enclosed dendrite and one attached to the ECM. The crossing on the v'ada/vdaB interface is also mostly rescued and all non-contacting crossing is eliminated.

(Han et al., 2012)

stan^E59/fry¹ double heterozygous larvae show increased crossing of dendrites in the dendritic arbor of the ddaC class IV neurons compared to wild type (neither single heterozygote shows this phenotype).

fry¹/Vang^stbm-6 double heterozygous larvae show increased crossing of dendrites in the dendritic arbor of the ddaC class IV neurons compared to wild type (neither single heterozygote shows this phenotype).

(Matsubara et al., 2011)

Transheterozygotes for fry¹/hpo^MGH4 exhibit obvious iso-neuronal as well as hetero-neuronal tiling defects, including a significant increase in dendritic crossing-points compared to single mutants.

(Emoto et al., 2006)

A fry¹/+ background enhances the multiple wing hair phenotype of flies that express either trc^{S292A+T453A.Scer\UAS}, trc^{S292A.Scer\UAS} or trc^{S292A+T453A.Scer\UAS} under the control of Scer\GAL4^ap-md544.

(He et al., 2005)

fry¹ trc^S066917a mats^e03077 triple heterozygotes have a weak multiple wing hair phenotype in 40% of wings.

(He et al., 2005)

fry¹/trc¹ larvae exhibit tiling defects, though they have normal dendritic length and branch points.

(Emoto et al., 2004)

mwh^unspecified fry¹ double mutant clones have a much stronger multiple wing hair phenotype (16.35 hairs/cell) than either single mutant. fry¹ fy^unspecified double mutant clones in the wing have a much stronger phenotype than either single mutant. fry¹ frtz^unspecified double mutant clones in the wing have a much stronger phenotype than either single mutant. trc^S066917a fry¹ double mutant clones do not have a stronger multiple wing hair phenotype (5.37 hairs/cell) than either single mutant.

(Cong et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

fry²/fry¹ is partially rescued by Scer\GAL4^Act.PU/fry^{UAS.Tag:MYC.GFP}

(Fang et al., 2010)

Not rescued by

fry²/fry¹ is not rescued by Scer\GAL4^Act.PU/fry^{1637.UAS.Tag:MYC}

(Fang et al., 2010)

Comments

(Fang et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32103

w^*; fry¹ P{white-un1}70C P{neoFRT}80B/TM6C, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fry¹

(Pan et al., 2024, Rui et al., 2020, Meltzer et al., 2016, Lee et al., 2015, Han et al., 2012, Horne-Badovinac et al., 2012, Matsubara et al., 2011, Fang et al., 2010, Parrish et al., 2009, Emoto et al., 2006, He et al., 2005)

fry^TAUS1

(He et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (15)

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