FlyBase Allele Report: Dmel\apt[167]

General Information

Symbol

Dmel\apt¹⁶⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0064958

Feature type

allele

Associated gene

Dmel\apt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: R110H. Missense mutation in the third exon substituting an A for G. This change occurs within a short repeat of amino acid sequence of acidic-Arg-Thr-X-Asn.

(Gellon et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,597,279..23,597,279 [+]

Nucleotide change:

G23597279A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

R110H

(Gellon et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | adult stage (with apt^KG05830)

(Starz-Gaiano et al., 2008)

abnormal cell migration | adult stage | somatic clone

(Starz-Gaiano et al., 2008)

lethal (with apt^13-66)

(McClure and Heberlein, 2013)

lethal (with apt^GS11714)

(McClure and Heberlein, 2013)

lethal | recessive

(Su et al., 1999, Gellon et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

apt¹⁶⁷/+ mutant adults do not exhibit any obvious wing defects.

(Wang et al., 2017)

In ~80% of homozygous apt^KG05830/apt¹⁶⁷ mutant stage 10 egg chambers, 1-7 additional invasive "stretched border cells" are seen, often dramatically stretched out and trailing behind the main cluster.

Egg chambers containing homozygous apt¹⁶⁷ mutant clones in stage 10 egg chambers contain 1-7 additional invasive "stretched border cells", often dramatically stretched out and trailing behind the main cluster. This phenotype is observed even if only a few anterior follicle cells are mutant, and no stretched border cells are seen in wild type cells. No phenotypes are seen when in egg chambers where the border cells are the only mutant cells.

(Starz-Gaiano et al., 2008)

Females with homozygous germline clones have phenotypically wild-type ovaries but a small fraction of the eggs laid develop into embryos with head defects.

(Lie and Macdonald, 1999)

Homozygotes die as late embryos or early larvae and show a similar phenotype to apt⁴¹ with respect to heart development and function.

(Su et al., 1999)

Homozygous embryos have severe defects in structures derived from the gnathal segments. The lateral bars of the H-piece and hypostomal sclerites are missing and the lateralgraten are truncated. The ectostomal sclerite and the cross bar of the H-piece are unaffected. The heads are open anteriorly and the dorsal pouch is reduced, replaced by sclerotic rubble. The dorsal bridge is missing or broken.

(Gellon et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

apt^KG05830/apt¹⁶⁷ has abnormal cell migration | adult stage phenotype, suppressible | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Starz-Gaiano et al., 2008)

Enhancer of

Statement

Reference

apt¹⁶⁷/apt[+] is an enhancer of abnormal size phenotype of hh^bar3

(Wang et al., 2017)

Other

Statement

Reference

apt¹⁶⁷/apt[+], hh^bar3 has visible phenotype

(Wang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

apt^KG05830/apt¹⁶⁷ has border follicle cell | oogenesis stage S10 phenotype, suppressible | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Starz-Gaiano et al., 2008)

apt^KG05830/apt¹⁶⁷ has egg chamber | oogenesis stage S10 phenotype, suppressible | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Starz-Gaiano et al., 2008)

Enhancer of

Statement

Reference

apt¹⁶⁷/apt[+] is an enhancer of wing phenotype of hh^bar3

(Wang et al., 2017)

Other

Statement

Reference

Socs36E¹⁷⁸, apt¹⁶⁷/apt[+] has border follicle cell phenotype

(Monahan and Starz-Gaiano, 2013)

Socs36E^EY06665/Socs36E¹⁷⁸, apt^KG05830/apt¹⁶⁷ has border follicle cell phenotype

(Monahan and Starz-Gaiano, 2013)

apt¹⁶⁷, bru1^QB has embryonic head phenotype

(Lie and Macdonald, 1999)

apt¹⁶⁷, bru1^QB, nanos^L7 has embryonic head phenotype

(Lie and Macdonald, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of the apt¹⁶⁷/+ mutation enhances the severity of the wing defects seen in hh^bar3/hh^bar3 mutant adults, showing further reduced wing area between L3 and L4, small wings and blistering.

(Wang et al., 2017)

Egg chambers of Socs36E¹⁷⁸/+, apt¹⁶⁷/+ double heterozygous females contain additional invasive cells that are not associated with the border cell cluster: 55% of the double heterozygous egg chambers contain more than one additional invasive cell compared to 10% and 15% respectively for each single heterozygote.

In 44% of Socs36E¹⁷⁸ apt^KG05830/Socs36E^EY06665 apt¹⁶⁷ stage 10 egg chambers the border cells are tethered to the anterior end of the egg chamber by non-cluster associated invasive cells. This inability to detach from neighbouring follicle cells results in a migration delay in 31% of the stage 10 double mutant egg chambers.

(Monahan and Starz-Gaiano, 2013)

One copy of Stat92E⁰⁶³⁴⁶ partially suppresses the invasive border cell phenotype seen in apt^KG05830/apt¹⁶⁷ mutant stage 10 egg chambers. A reduction is seen in both the frequency of affected egg chambers and the number of cells. On average 26% of egg chambers contain "stretched border cells" compared to ~80% in apt^KG05830/apt¹⁶⁷ alone. The border cell cluster also detaches much more easily from the anterior end of the egg chamber.

(Starz-Gaiano et al., 2008)

Embryos derived from a cross of wild-type males to females heterozygous for aret^QB and also heterozygous for apt¹⁶⁷ occasionally have head defects (these defects are not seen in embryos derived from females singly heterozygous for aret^QB or apt¹⁶⁷). This phenotype is largely suppressed if the females are also heterozygous for nos^L7.

(Lie and Macdonald, 1999)

Enhances the lethality of Dfd¹³/Dfd³ flies grown at 29^oC - leaving nearly zero survivors.

(Gellon et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

(Su et al., 1999)

(Su et al., 1999)

(Su et al., 1999)

(Gellon et al., 1997)

apt⁴¹

(Gellon et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

One of three alleles isolated in this screen.

(Gellon et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

apt167

(Starz-Gaiano et al., 2008)

apt¹⁶⁷

(Marini-Davis et al., 2025, Wang et al., 2017, Monahan and Starz-Gaiano, 2016, McClure and Heberlein, 2013, Monahan and Starz-Gaiano, 2013, Yoon et al., 2011, Starz-Gaiano et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (10)

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