FlyBase Allele Report: Dmel\CycB[S.hs]

General Information

Symbol

Dmel\CycB^S.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0065305

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

P{HS-CycB^S}

Key Links

Transgenic product class

inframe_deletion

(Sprenger et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Sprenger et al., 1997)

Mutagen

in vitro construct

(Su et al., 1998, Sprenger et al., 1997, Su and O'Farrell, 1997)

Progenitor genotype

Carried in construct

P{HS-CycB^S}

Cytology

Description

A CycB cDNA lacking sequences encoding the destruction box is expressed under the control of a heat shock promoter.

(Sprenger et al., 1997)

A deleted form of CycB (lacking amino acids 3 to 47 and with a Gly residue as the second amino acid residue) is expressed under the control of an Hsp70 promoter. The deletion removes the "destruction box" and results in a stable CycB protein being produced.

(Su and O'Farrell, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

CycB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Echard and O'Farrell, 2003)

abnormal mitotic cell cycle

(Parry et al., 2003)

Phenotype Manifest In

anaphase & condensed nuclear chromosome

(Parry and O'Farrell, 2001)

cell cycle

(Parry and O'Farrell, 2001)

kinetochore

(Parry et al., 2003)

mitotic anaphase

(Parry and O'Farrell, 2001)

Detailed Description

Statement

Reference

Expression of CycB^S.hs results in mitotic catastrophe in larval neuroblasts, including multinucleation and drastically increased cell diameter. Some loss of neuroblasts is seen.

(Kuang et al., 2014)

When CycB^S.hs is induced during the G2 phase of cycle 14, cytokinesis is not initiated.

(Echard and O'Farrell, 2003)

In embryos expressing CycB^S.hs, merotelic attachments are seen; i.e. kinetochore fibres are seen to extend from both poles to the pseudometaphase plate. Most but not all cells are fully arrested. Those that are not exhibit slow mitotic progress with a high frequency of chromosome segregation anomalies.

(Parry et al., 2003)

Heat shock expression of CycB^S.hs produces embryos with cells arrested in an abnormal metaphase. Disjoined chromosomes can be seen scattered along the mitotic spindle. Progression through prophase and metaphase appears normal. Sister chromatids appear to disjoin and move toward opposite poles normally, albeit slowly. As the trailing tips of the longer chromosomes lose contact with their sisters, however, anaphase movement stops. After a pause, chromosomes move slowly along chaotic paths back to the midpoint of the spindle taking erratic and independant excursions towards the poles. The rate of this poleward movement varies from chromosome to chromosome. Excursions which are led by the kinetochore stop abruptly before reaching the pole, after which the chromosome drifts slowly back to the middle of the spindle in a movement not led by the kinetochore. Sometimes this gradual movement is interrupted by a sudden kinetochore led acceleration toward the opposite pole that typically carries the chromosome across the middle of the spindle and close to the other pole. Expression retards chromosome separation, slows chromosome movement and reduces the final extent of separation. Also the separation of the spindle poles during anaphase B is not seen.

(Parry and O'Farrell, 2001)

S phase is not induced in embryos when cdc2^AF.hs is co-expressed with CycB^S.hs using heat shock.

(Sprenger et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

CycA^S.hs, CycB^S.hs has abnormal mitotic cell cycle phenotype

(Vidwans et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

CycB^S.hs has cell cycle phenotype, enhanceable by Cdk1^hs.PS

(Parry and O'Farrell, 2001)

Other

Statement

Reference

Cdk1^AF.hs, CycB^S.hs has mitotic cell cycle phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^S.hs has mitotic domain 4 phenotype

(Su et al., 2000)

CycA^S.hs, CycB^S.hs has mitotic cell cycle phenotype

(Vidwans et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-induction of cdc2^hs.PS and CycB^S.hs leads to a more metaphase arrest than seen in CycA^S.hs embryos alone.

(Parry and O'Farrell, 2001)

Many cells of heatshocked embryos carrying cdc2^AF.hs and CycB^S.hs fail to delay mitosis after irradiation.

(Su et al., 2000)

Expression of both CycA^S.hs and CycB^S.hs in embryos using heat shock results in most epidermal cells being arrested in mitosis 2 hours after the heat shock. No more than 2 centrosomes are seen at each spindle pole. All centriole pairs are disengaged and the centrioles are not associated with procentrioles.

(Vidwans et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

B^S

(Su and O'Farrell, 1997)

CycB^S.hs

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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