FlyBase Allele Report: Dmel\Cdk1[AF.hs]

General Information

Symbol

Dmel\Cdk1^AF.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0064930

Feature type

allele

Associated gene

Dmel\Cdk1

Associated Insertion(s)

Carried in Construct

P{HS-Cdk1^AF}

Also Known As

Cdk1AF, Hs-Cdk1AF

Key Links

Transgenic product class

missense_variant

(Sprenger et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Sprenger et al., 1997)

Mutagen

in vitro construct

(Su et al., 1998, Sprenger et al., 1997)

Progenitor genotype

Carried in construct

P{HS-Cdk1^AF}

Cytology

Description

Construct: Amino acid replacement: T?A. Amino acid replacement: Y?F. The mutant form of cdc2 is expressed using a heat shock promoter.

(Sprenger et al., 1997)

Allele components

Component

Use(s)

Encoded product / tool

Cdk1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | heat sensitive

(Gawlinski et al., 2007)

female sterile

(Stumpff et al., 2005)

increased cell number | spermatogenesis

(Jin et al., 2005)

increased occurrence of cell division | spermatogenesis

(Jin et al., 2005)

lethal | heat sensitive

(Lane et al., 2000)

Phenotype Manifest In

cyst cell of testis

(Jin et al., 2005)

mitotic cell cycle

(Jin et al., 2005)

secondary spermatogonium

(Jin et al., 2005)

Detailed Description

Statement

Reference

Expression of cdc2^AF.hs using heat shock results in induction of premature mitosis in embryos.

(Gawlinski et al., 2007)

Heat-induced expression of cdc2^AF.hs in testes results in germline overproliferation defects, including an increase in secondary spermatogonia, due to an extra round of mitosis. Expression of cdc2^AF.hs also results in an increase in somatic cyst cells.

(Jin et al., 2005)

Females fail to lay eggs following induction of cdc2^AF.hs.

(Stumpff et al., 2005)

Expression of cdc2^AF.hs using heat shock results in unhatched eggs.

(Lane et al., 2000)

Many cells of heatshocked embryos carrying cdc2^AF.hs and either CycB^S.hs, CycA^hs.PK or CycB^hs.PK fail to delay mitosis after irradiation.

(Su et al., 2000)

Has no effect on S phase induction in embryos when expressed using heat shock.

(Sprenger et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cdk1^AF.hs has abnormal mitotic cell cycle | heat sensitive phenotype, suppressible by Scer\GAL4^prd.RG1/Z600^UAS.cGa

(Gawlinski et al., 2007)

NOT Suppressor of

Statement

Reference

Cdk1^AF.hs is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycA^C8LR1

(Reber et al., 2006)

Cdk1^AF.hs, CycB^hs.PK, CycB3^hs.PS is a non-suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of CycA^C8LR1

(Reber et al., 2006)

Other

Statement

Reference

Cdk1^AF.hs, CycA^C8LR1, CycB3^Δ, CycB^Δ90 has abnormal mitotic cell cycle | embryonic stage phenotype

(Reber et al., 2006)

Cdk1^AF.hs, CycA^hs.PK has increased occurrence of cell division | embryonic stage phenotype

(Su et al., 1998, Sprenger et al., 1997)

Cdk1^AF.hs, CycB^hs.PK has increased occurrence of cell division | embryonic stage phenotype

(Su et al., 1998)

Cdk1^AF.hs, CycA^S.hs has increased occurrence of cell division | embryonic stage phenotype

(Sprenger et al., 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cdk1^AF.hs, CycA^hs.PK has mitotic cell cycle phenotype, suppressible by rux^hs.PT

(Foley et al., 1999)

Suppressor of

Statement

Reference

Cdk1^AF.hs/rux^hs.PT is a suppressor of mitotic cell cycle phenotype of CycA^hs.PK

(Foley et al., 1999)

NOT Suppressor of

Statement

Reference

Cdk1^AF.hs is a non-suppressor of embryonic epidermis phenotype of CycA^C8LR1

(Reber et al., 2006)

Cdk1^AF.hs, CycB^hs.PK, CycB3^hs.PS is a non-suppressor of embryonic epidermis phenotype of CycA^C8LR1

(Reber et al., 2006)

Other

Statement

Reference

Cdk1^AF.hs, CycA^C8LR1, CycB3^Δ, CycB^Δ90 has embryonic epidermis phenotype

(Reber et al., 2006)

Cdk1^AF.hs, CycA^hs.PK has mitotic cell cycle phenotype

(Su et al., 2000, Foley et al., 1999)

Cdk1^AF.hs, CycA^hs.PK has mitotic domain 4 phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^S.hs has mitotic cell cycle phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^S.hs has mitotic domain 4 phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^hs.PK has mitotic cell cycle phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycB^hs.PK has mitotic domain 4 phenotype

(Su et al., 2000)

Cdk1^AF.hs, CycA^hs.PK has primordial germ cell phenotype

(Su et al., 1998)

Cdk1^AF.hs, CycB^hs.PK has primordial germ cell phenotype

(Su et al., 1998)

Cdk1^AF.hs, CycA^hs.PK has embryonic epidermis phenotype

(Sprenger et al., 1997)

Cdk1^AF.hs, CycA^S.hs has embryonic epidermis phenotype

(Sprenger et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Z600^Scer\UAS.cGa under the control of Scer\GAL4^prd.RG1 almost completely inhibits the ectopic mitosis induced in embryos by expression of cdc2^AF.hs using heat shock.

(Gawlinski et al., 2007)

Expression of cdc2^AF.hs in CycA^C8LR1 mutant embryos fails to restore mitosis 16 in the epidermis.

Coexpression of three transgenes, cdc2^AF.hs, CycB^Δ90, and CycB3^Δ, restores the entry of epidermal cells in CycA^C8LR1 embryos into mitosis 16. After entry into mitosis, the epidermal cells are unable to complete this division. However, coexpression of cdc2^AF.hs, CycB^hs.PK and CycB3^hs.PS does not suppress this phenotype.

(Reber et al., 2006)

Expression of cdc2^AF.hs in a stg² mutant background drives the G2-arrested stg mutant cells into mitosis.

(Su et al., 2001)

Simultaneous over-expression of CycA^hs.PK and cdc2^AF.hs is sufficient to induce mitosis throughout the entire embryo. This phenotype is suppressed by the addition of rux^hs.PT.

(Foley et al., 1999)

Heat induced coexpression of CycA^hs.PK and Cdk1^AF.hs or CycB^hs.PK and Cdk1^AF.hs induces proliferation in quiescent pole cells. Ectopic mitoses are followed by cell division in pole cells.

(Su et al., 1998)

Dramatically increases the efficiency of S phase induction in embryos when co-expressed with either CycA^hs.PK or CycA^S.hs using heat shock. S phase is not induced in embryos when cdc2^AF.hs is co-expressed with either CycB^hs.PK or CycB^S.hs using heat shock.

(Sprenger et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cdk1^AF.hs

Cdk1AF

(Stumpff et al., 2005)

cdc2^AF.hs

Name Synonyms

Secondary FlyBase IDs

References (15)

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